Muhammad Shafiq, Muhammad A. Khan, Muhammad Hussain, Muhammad Sajjad, Ayaz Farzand, Anam Moosa, Abdul Jabbar, Abdur R. Khan, Sohail Akhtar, Rana Binyamin


Ustilago tritici, a seed borne pathogen, remains dormant in seed embryo and causes loose smut disease. The present study was conducted to evaluate two inoculation methods i.e., dip inoculation and syringe inoculation to find out the better method for efficient and reproducible assessment of disease severity, disease incidence and screening of resistant wheat germplasm. Syringe inoculation was found to be better for the inoculation of heads with higher disease incidence than dip inoculation method. Twenty-seven lines were tested for their resistance against loose smut. Two lines were moderately resistant, nineteen lines susceptible and three lines were highly susceptible. Environmental conditions play an integral role in the development of loose smut disease. The relationship of temperature, humidity and rainfall with loose smut development was studied. An average temperature of 23-27 °C, relative humidity 60-90% and rainfall 0.02-1 mm rainfall were statistically most conducive for disease development. The correlation of environmental factors significantly influenced the disease development.


Ustilago tritici, inoculation, temperature, relative humidity, rainfall, resistant, susceptible.

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Druzhin, A.E. and V.A. Krupnov. 2000. Influence of environmental conditions for infection of spring wheat by loose smut in Volga region. Doklady Aossiiskoi academii selskokhozyaistvennykh nauk. 2:16-18.

Gondal, A.S. and A.R. Khan. 2012. Research Overview of Barani Agricultural Research Institute. Lap Lamb. Acad. Publ. Germany. 18-19.

FAOSTAT. 2014. FAOSTAT statistical database collection.

Hassan, S.F., N.A. Khan, M. Arshad, Anwar and Mahmood. 1970. A note on occurrence of physiological race 2 of Ustilag tritici (Pers) Roster. West Pakistan. J. Agric. Res.8:218-219.

Ilyass, M.B., M.I. Ahmad and M.A. Bajwa. 1990. Evaluation of inoculation methods and screening of wheat germplasm against loose smut of wheat. Pak. J. Agric. Sci. 27:252-256.

Jamil, S.A., M. Jalaluddin and A. Ghaffar, 2005. Major seed-borne diseases of wheat in Pakistan. Status of plant Pathology in Pakistan, University of Karachi, Pakistan. 111-115.

Joshi, L.M., D.V. Singh and K.D. Srivastava. 1985. Monitoring of wheat disease during 1984-1985. In: Proc. 24th All India Wheat Res. Workers' Workshop, GBPUAT.

Mishra, R.P., S.P. Tiwari and M.N. Khare. 1990. Studies on loose smut of wheat. Testing of resistance and susceptibility for wheat varieties to Ustilago tritici (pers) Rostr under artificial inoculation. Ind. J. Myco. Pl. Pathol. 20:171-173.

Neelam, R.K. Sahu and A. Srinivasaraghavan. 2012. Role of weather parameters on incidence and development of sugarcane smut disease. Quat. J. Life Sci. 9:497-499.

Paul, P.A. and G.P. Munkvold. 2005. Influence of temperature and relative humidity on sporulation of Cercospora zeae-maydis and expansion of gray leaf spot lesions on maize leaves. Plant Dis. 89:624-630.

Poehlman, J.M. 1945. A simple method of inoculating barley with loose smut. Phytopath. 35:457-460.

Seth, R.K., A. Shah and D.N. Shukla. 2014. Screening of disease resistant varieties against loose smut of wheat. J. Nat. Prod. Pl. Res. 4:49.

Shaner, G. and R.E. Finney. 1977. The Effect of Nitrogen Fertilization on the Expression of Slow Mildewing Resistance in Knox Wheat. Phytopathol. 67:1051-1056.

Steel, R.G.O., J.H. Torrie and D. Dickey. 1997. Principles and Procedures of Statistics. A Biometrical Approach. 3rd Edn. Mc Graw- Hill, NY, U.S.A.


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