VARIETAL RESPONSE OF BITTER GOURD GERMPLASM AGAINST MYROTHECIUM RORIDUM TODE EX. FR. CAUSING MYROTHECIUM LEAF SPOT DISEASE AND ITS CHEMOSYNTHETIC MANAGEMENT

Hasnain Sajjad, Muhammad Iqbal, Abdul Rehman, Muhammad W. Alam, Saira Mehbob, Muhammad Idrees

Abstract


The fungus Myrothecium roridum is the chief hazard to crops including cucurbits inhabit in soil as saprophyte, on diseased plant debris and also as facultative parasite on vegetables, fruits and ornamental plants. Keeping in view the damage caused by M. roridum to bitter gourd, screening of nine bitter gourd varieties (BG34F1, Manika 7004, CBT 36, BSS 616, KHBG 037, Leena 7005, Raja, Tipu and CO2NO3) was done in Plant Pathology Research Institute, Ayub Agricultural Research Institute Faisalabad under field conditions. All the varieties showed moderately susceptible to highly susceptible reaction ranging between 25-75% infections on leaves. Significant disease severity was recorded in the genotype Tipu while the lowest disease severity was recorded in the genotype BG34F1. Maximum numbers of flowers were recorded in the variety Tipu (24.33) followed by Raja (17.33) and CO2NO3 (15.33). Maximum number of fruits were recorded in the variety Tipu (40.00) followed by BSS 616 (11.00) and CO2NO3 (10.33). M. roridum from the diseased samples was isolated and identified morphologically as white cylindrical conidia with rounded ends under the microscope. In vitro management of the fungus M. roridum was done with fungicides having novel mode of action including Antracol® (Propineb), Cabrio Top® (Pyraclostrobin + Metriam Complex), Nativo®(Tebuconazole + Trifloxystrobin), Topsin M® (Thiophenate Methyl) and Score® (Difenoconazole) at concentrations of 50,100,150,200,250 µg/mL to inhibit the mycelial growth. Among all the fungicides tested, Score significantly reduced the mycelial growth of M. roridum with a value of 49% decrease over control followed by Nativo and Topsin M with a value of 48% and 47% respectively. Antracol was found to be the least effective in reducing the mycelial growth of M. roridm with a value of 22% decrease over control.


Keywords


Bitter gourd, Myrothecium leaf spot, varietal response, Difenoconazole, (Tebuconazole+Trifloxystrobin)

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