CHEMICAL COMPOSITION, ANTIBACTERIAL ACTIVITY OF ESSENTIAL OILS AND LEAF EXTRACTS OF MENTHA PULEGIUM (L) AND GLYCYRRHIZA FOETIDA (DESF) AGAINST THE PHYTOPATHOGENIC BACTERIA

Hanane Lougraimzi, Aghraz Abdellah, Bouaichi Abdelaaziz, El J. Meryem, Achbani E. Hassan

Abstract


This research was conducted to evaluate the components of the Mentha pulegium (L) and Glycyrrhiza foetida (Desf) that were extracted by hydro distillation and analyzed using GC-MS methods. They are spontaneous plants widespread in Gharb of Morocco. The aim of the study is to investigate the antibacterial activity of the essential oils and leaf methanolic extracts of these two medicinal plants against five phytopathogenic bacteria. The evaluation of the antimicrobial activity of the two species by estimating the diameter of the inhibition zone has shown that the essential oil of M. pulegium exhibited a higher antimicrobial activity than G. foetida which varied according to the sensitivity of the phytopathogenic strains. The results obtained revealed different degrees of sensitivity toward methnolic extract. However, the methanolic extract and the essential oil of M. pulegium are more active based on the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). The antimicrobial activities of leaf extracts from the two plants highlighted in this study could justify their therapeutic use. These results should be strongly recommended as an alternative to a chemical compound that still represents the problem of toxic residues.


Keywords


Essential oils; Medicinal plant; Antibacterial activity; Leaves extracts; Phytopathogenic bacteria.

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References


Ait-Ouazzou, A., S. Lorán, M. Bakkali, A. Laglaoui, C. Rota, A. Herrera, R. Pagán and P. Conchello. 2011. Chemical composition and antimicrobial activity of essential oils of Thymus algeriensis, Eucalyptus globulus and Rosmarinus officinalis from Morocco. Journal of the Science of Food and Agriculture, 91: 2643-2651.

Ali, E. M. 2013. Phytochemical composition, antifungal, antiaflatoxigenic, antioxidant, and anticancer activities of Glycyrrhiza glabra L. and Matricaria chamomilla L. essential oils. Journal of medicinal plants research 729: 2197-2207.

Bouyahya, A., A. Et-Touys, Y. Bakri, A. Talbaui, H. Fellah, J. Abrini and N. Dakka. 2017. Chemical composition of Mentha pulegium and Rosmarinus officinalis essential oils and their antileishmanial, antibacterial and antioxidant activities. Microbial Pathogenesis, 111: 41-49.

Chouitah, O., B. Meddah, A. Aoues and P. Sonnet. 2011. Chemical composition and antimicrobial activities of the essential oil from Glycyrrhiza glabra leaves. Journal of Essential Oil Bearing Plants, 14: 284-288.

Daferera, D. J., B. N. Ziogas and M. G. Polissiou. 2003. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis. Crop Protection, 22: 39-44.

Díaz-Maroto, M. C., N. Castillo, L. Castro-Vázquez, M. Ángel González-Viñas and M. S. Pérez-Coello. 2007. Volatile composition and olfactory profile of pennyroyal (Mentha pulegium L.) plants. Flavour and Fragrance Journal, 22: 114-118.

Falleh, H., R. Ksouri, K. Chaieb, N. Karray-Bouraoui, N. Trabelsi, M. Boulaaba and C. Abdelly. 2008. Phenolic composition of Cynara cardunculus L. organs, and their biological activities. Comptes Rendus Biologies, 331: 372-379.

Ghourri, M., L. Zidane and A. Douira. 2014. Catalogue des plantes médicinales utilisées dans le traitement de la lithiase rénale dans la province de Tan-Tan (Maroc saharien). International Journal of Biological and Chemical Sciences, 7: 1688.

Hmiri, S., M. Rahouti, Z. Habib, B. Satrani, M. Ghanmi and M. El Ajjouri. 2011. Évaluation du potentiel antifongique des huiles essentielles de Mentha pulegium et d’ Eucalyptus Camaldulensis dans la lutte biologique contreles champignons responsables de la détérioration des pommes en conservation. Bulletin de la société royale des sciences de liège.

Ismaili, H., L. Milella, S. Fkih-Tetouani, A. Ilidrissi, A. Camporese, S. Sosa, G. Altinier, R. Della Loggia and R. Aquino. 2004. In vivo topical anti-inflammatory and in vitro antioxidant activities of two extracts of Thymus satureioides leaves. Journal of Ethnopharmacology, 91: 31-36.

Jafarpour, M., A. R. Golparvar and A. Lotfi. 2013. Antibacterial activity of essential oils from Thymus vulgaris, Trachyspermum ammi and Mentha aquatica against Erwinia carotovora in vitro. Journal of Herbal Drugs (An International Journal on Medicinal Herbs), 4: 115-118.

Jamila, F. and E. Mostafa. 2014. Ethnobotanical survey of medicinal plants used by people in Oriental Morocco to manage various ailments. Journal of Ethnopharmacology, 154: 76-87.

Jirovetz, L., S. Bail, G. Buchbauer, Z. Denkova, A. Slavchev, A. Stoyanova, E. Schmidt and M. Geissler. 2006. Antimicrobial testings, gas chromatographic analysis and olfactory evaluation of an essential oil of hop cones (Humulus lupulus L.) from Bavaria and some of its main compounds. Scientia Pharmaceutica, 74: 189.

Kanakis, C. D., E. A. Petrakis, A. C. Kimbaris, C. Pappas, P. A. Tarantilis and M. G. Polissiou. 2012. Classification of Greek Mentha pulegium L. (Pennyroyal) samples, according to geographical location by Fourier transform infrared spectroscopy. Phytochemical analysis, 23: 34-43.

Mahboubi, M. and G. Haghi. 2008. Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. Journal of Ethnopharmacology, 119: 325-327.

Maisuthisakul, P., M. Suttajit and R. Pongsawatmanit. 2007. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chemistry, 100: 1409-1418.

Majhenič, L., M. Škerget and Ž. Knez. 2007. Antioxidant and antimicrobial activity of guarana seed extracts. Food Chemistry, 104: 1258-1268.

Messiaen, C.-M., D. Blancard and F. Rouxel. 1991. Les maladies des plantes maraîchères, 3e éd. Editions Quae.

Paulin, J.-P., M. Ridé and J.-P. Prunier. 2001. Découverte des bactéries phytopathogènes il y a cent ans : controverses et polémiques transatlantiques. Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie, 324: 905-914.

Popoff, M. Y., K. Kersters, M. Kiredjian, I. Miras and C. Coynault. 1984. Position taxonomique de souches de Agrobacterium d'origine hospitalière. Annales de l'Institut Pasteur / Microbiologie, 135: 427-442.

Qi, X.-L., T.-T. Li, Z.-F. Wei, N. Guo, M. Luo, W. Wang, Y.-G. Zu, Y.-J. Fu and X. Peng. 2014. Solvent-free microwave extraction of essential oil from pigeon pea leaves [Cajanus cajan (L.) Millsp.] and evaluation of its antimicrobial activity. Industrial Crops and Products, 58: 322-328.

Remmal, A., T. Bouchikhi, K. Rhayour, M. Ettayebi and A. Tantaoui-Elaraki. 1993. Improved method for the determination of antimicrobial activity of essential oils in agar medium. Journal of Essential Oil Research, 5: 179-184.

Scortichini, M. and M. Rossi. 1992. In vitro behaviour of Erwinia amylovora towards some natural products showing bactericidal activity. VI International Workshop on Fire Blight 338. pp. 191-198.

Shakeri, A., J. Akhtari, V. Soheili, S. F. Taghizadeh, A. Sahebkar, R. Shaddel and J. Asili. 2017. Identification and biological activity of the volatile compounds of Glycyrrhiza triphylla Fisch. & C.A. Mey. Microbial Pathogenesis, 109: 39-44.

Shirazi, F. H., N. Ahmadi and M. Kamalinejad. 2004. Evaluation of northern Iran Mentha pulegium L. cytotoxicity. DARU Journal of Pharmaceutical Sciences, 12: 106-110.

Song, A., Y. Wang and Y. Liu. 2009. Study on the chemical constituents of the essential oil of the leaves of Eucalyptus globulus Labill from China. Asian Journal of Traditional Medicines, 4: 34-140.

Sutour, S. 2010. Etude de la composition chimique d'huiles essentielles et d'extraits de menthe de Corse et de Kumquats. Université de Corse.

Teixeira, B., A. Marques, C. Ramos, I. Batista, C. Serrano, O. Matos, N. R. Neng, J. M. F. Nogueira, J. A. Saraiva and M. L. Nunes. 2012. European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Industrial Crops and Products, 36: 81-87.

Trigui, M., A. Ben Hsouna, I. Hammami, G. Culioli, M. Ksantini, S. Tounsi and S. Jaoua. 2013. Efficacy of Lawsonia inermis leaves extract and its phenolic compounds against olive knot and crown gall diseases. Crop Protection, 45: 83-88.

Valnet, J. 1975. Aromatherapie: Traitement des maladies par les essences des plantes (7. revue, corr. et augm. ed.). Paris, France: Maloine. Includes Bibliography: 429-434.

Yegen, O., A. Unlu and B. Berger. 1998. Use and side effects on the soil microbial activity of the essential oil from Thymbra spicata to control pepper blight Phytophthora capsici. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, 105: 602-610.

Youbi, A., I. Ouahidi, L. Mansouri, A. Daoudi and D. Bousta. 2016. Ethnopharmacological survey of plants used for immunological diseases in four regions of Morocco. European Journal of Medicinal Plants, 13: 1-24.

Zanjani, K., N. Mohammadi, M. Zojaji and H. Bakhoda. 2015. Chemical Composition of the Essential Oil of Mentha pulegium L. and its Antimicrobial Activity on Proteus mirabilis, Bacillus subtilis and Zygosaccharomyces rouxii. Journal of Food Biosciences and Technology, 5: 31-40.




DOI: https://doi.org/10.33866/phytopathol.031.01.0497

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