MORPHOLOGICAL AND MOLECULAR VARIATION OF PHYTOPHTHORA COLOCASIAE IN ‘SATOIMO’ TARO (COLOCASIA ESCULENTA VAR ANTIQUORUM) IN INDONESIA

Eka Wisdawati, Tutik Kuswinanti, Ade Rosmana, Andi Nasruddin

Abstract


Taro leaf blight is a harmful disease caused by Phytophthora colocasiae Raciborski and has become the primary factor in damaging ‘satoimo' taro. A survey conducted in the agriculture fields of satoimo taro (Colocasia esculenta var antiquorum) in Indonesia indicated its severity disease scale between 25-50%. Morphological and molecular techniques were used to characterized the population of Phytophthora colocasiae obtained from different locations in Makassar, Maros and Gowa districts. Based on colony growth patterns, the isolates exhibited stellate and petaloid. The mean sporangial length of isolates of P. colocasiae range from 37.06  and 53.80 µm and mean width range from 22.27 and 26.90 µm. P. colocasiae are highly dynamic in nature and a considerable degree of diversity exists among them.  This provides an important basis for developing suitable control against leaf blight of taro caused by P. colocasiae based on good integrated approach. Based on the phylogenetic analysis, P. colocasiae isolates (PCEMK01 and PCEMR01) was exhibited in the same cluster with P. colocasiae strain 3 reported from India with the bootstrap of 96% at 500 replicates in MEGA 5.


Keywords


Taro leaf blight, Satoimo Taro, Phytophthora colocasiae

Full Text:

PDF

References


Adomako, J., C. Kwoseh, E. Moses and R. N. A. Prempeh. 2018. Variations in morphological and molecular characteristics of Phytophthora colocasiae population causing leaf blight of taro in Ghana. Archives of Phytopathology and Plant Protection, 51: 1009-1021.

Bandyopadhyay, R., K. Sharma, T. J. Onyeka, A. Aregbesola and P. L. Kumar. 2011. First Report of Taro (Colocasia esculenta) Leaf Blight Caused by Phytophthora colocasiae in Nigeria. Plant Disease, 95: 618-618.

Drenth, A. and B. Sendall. 2001. Practical guide to detection and identification of Phytophthora. Tropical Plant Protection, 1: 32-33.

George, A., M. L. Jeeva, V. S. Nath, G. L. Sreelatha and M. G. Sujina. 2018. Simple and efficient genomic DNA extraction protocol for molecular characterisation of Phytophthora colocasiae causing taro leaf blight. Archives of Phytopathology and Plant Protection, 51: 241-251.

Goodwin, S. B. 1997. The Population Genetics of Phytophthora. Phytopathology, 87: 462-473.

Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic acids symposium series No: 41. Information Retrieval Ltd, pp. 95-98.

Lebot, V., C. Herail, T. Gunua, J. Pardales, M. Prana, M. Thongjiem and N. Viet. 2003. Isozyme and RAPD variation among Phytophthora colocasiae isolates from South-east Asia and the Pacific. Plant Pathology, 52: 303-313.

Mbong, G., C. Fokunang, E. Manju, A. Njukeng, E. Tembe-Fokunang and R. Hanna. 2015. Mycelia Growth and Sporulation of Phytophthora colocasiae Isolates under Selected Conditions. American Journal of Experimental Agriculture, 8: 193-201.

Mbong, G., C. Fokunang, L. Fontem, M. Bambot and E. Tembe. 2013. An overview of Phytophthora colocasiae of cocoyams: A potential economic disease of food security in Cameroon. Discourse Journal of Agriculture and Food Sciences, 1: 140-145.

Mishra, A. K., K. Sharma and R. S. Misra. 2010. Isozyme and PCR-based genotyping of epidemic Phytophthora colocasiae associated with taro leaf blight. Archives Of Phytopathology And Plant Protection, 43: 1367-1380.

Misra, R. S., A. K. Mishra, K. Sharma, M. L. Jeeva and V. Hegde. 2011. Characterisation of Phytophthora colocasiae isolates associated with leaf blight of taro in India. Archives of Phytopathology and Plant Protection, 44: 581-591.

Misra, R. S., K. Sharma and A. K. Mishra. 2008. Phytophthora leaf blight of Taro (Colocasia esculenta)—a review. Asian Australas J Plant Sci Biotechnol, 2: 55-63.

Nath, V. S., M. Senthil, V. M. Hegde, M. L. Jeeva, R. S. Misra, S. S. Veena and M. Raj. 2012. Genetic diversity of Phytophthora colocasiae isolates in India based on AFLP analysis. 3 Biotech, 3: 297-305.

Nath, V. S., V. M. Hegde, M. L. Jeeva, R. S. Misra, S. S. Veena, M. Raj and D. S. Sankar. 2014. Morphological, pathological and molecular characterization of Phytophthora colocasiae responsible for taro leaf blight disease in India. Phytoparasitica, 43: 21-35.

Nath. 2017. Survey penyakit hawar daun dan bercak oranye pada tanaman talas Colocasia esculenta di Malang (Brawijaya), Thesis abstract.

Omane, E., K. A. Oduro, E. W. Cornelius, I. Y. Opoku, A. Y. Akrofi, K. Sharma, P. L. Kumar and R. Bandyopadhyay. 2012. First Report of Leaf Blight of Taro (Colocasia esculenta) Caused by Phytophthora colocasiae in Ghana. Plant Disease, 96: 292-292.

Padmaja, G. 2013. Studies on Phytophthora leaf blight of taro Colocasia esculenta (L.) Schott.. Thesis submitted to Acharya N.G. Ranga Agricultural University.

Padmaja, G., G. Uma Devi, B. Kanaka Mahalakshmi and D. Sridevi. 2017. Characterization of Isolates of Phytophthora colocasiae Collected from Andhra Pradesh and Telangana Causing Leaf Blight of Taro. International Journal of Current Microbiology and Applied Sciences, 6: 1901-1912.

Saitou, N., and M. Nei, M. 1987. The neighbor-joining method : a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 4: 406–425.

Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar. 2011. MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28: 2731-2739.

Tariq, A., F. Naz, C. A. Rauf and G. Irshad. 2015. Long term and least expensive preservation methods for various fungal cultures. Pakistan journal of phytopathology, 27: 147-151.

Tsopmbeng, G. R., D. A. Fontem and K.F. Yamde. 2012. Evaluation of culture media for growth and sporulation of Phytophthora colocasiae Racib., causal agent of taro leaf blight. International Journal of Biological and Chemical Sciences, 6: 1566–1573.

Waterhouse, G. M. 1963. Key to the species of Phytophthora de Bary. Commonwealth Mycological Institute, Kew, UK, Mycol Papers, 92: 22.

White, T. J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. PCR Protocols: a guide to methods and applications, 18: 315-322.




DOI: https://doi.org/10.33866/phytopathol.032.01.0530

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Eka Wisdawati, Tutik Kuswinanti, Ade Rosmana, Andi Nasruddin

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

      
   
Pakistan Journal of Phytopathological
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.