CHARACTERIZATION OF FUSARIUM SOLANI, CAUSAL AGENT OF PEA WILT AND ITS BIO-MANAGEMENT

Asfand Iqbal, Gulshan Irshad, Farah Naz, Salman Ghuffar, Rabia Khursheed, Aamir Bashir, Muhammad Z. Mustafa, Ammar Mehmood, Najam U. Hassan

Abstract


Pea (Pisum Sativum L.) is an important winter crop in Pakistan. The quality and production of pea are deteriorated by several biotic and abiotic factors. Among the biotic factors, Fusarium solani is one of the major limiting factors in pea production. In current studies, a  total of two fields located in Koont Research Farm, PMAS-Arid Agriculture University Rawalpindi, and National Agricultural Research Centre Islamabad (NARC) were surveyed for the determination of Fusarium wilt disease incidence. Total eighteen isolates were collected during the field survey; a pathogenicity test was carried out to confirm the degree of virulence among all pathogens. A total of 03 plant essential oils such as Neem (Azadirachta indica), Eucalyptus (Eucalyptus globulus Labill) and Clove (Syzygium aromaticum (L.) at 200, 400 and 600 ppm were tested against F. solani causing Fusarium wilt of pea. Results revealed that eucalyptus essential oil at all applied concentrations showed the maximum effectiveness against mycelial growth of F. solani under vitro condition followed by neem and clove essential oil as compared to control. Keeping in view the above mentioned, eucalyptus essential oil can be used as bio fungicide against Fusarium solani causing Fusarium wilt of Pea.


Keywords


Pisum Sativum; Fusarium solani; Pea Wilt; Bio management

Full Text:

PDF

References


Batish, D.R., H.P. Singh, N. Setia, S. Kaur and R.K. Kohli, 2006. Chemical composition and phytotoxicity of volatile essential oil from intact and fallen leaves of Eucalyptus citriodora. Zeitschrift Naturforschung. 61: 465-471.

Booth, C. 1977. Fusarium: Laboratory guide to the identification of the major species. Commonwealth Mycological Institute, Kew Surrey, England. pp.31.

Cheesbrough, M. 2000. Microbiological test: District Laboratory Practice in Tropical Countries. In: Cremer, A. and Evan, G. (eds). Cambridge University Press, UK. 1-226 PP.

Clark, A. (ed.) 2007. Managing cover crops profitably. 3rd ed. Sustainable agriculture research and education program handbook series, bk 9. Sustainable Agriculture Research and Education, College Park, MD.]

FAO. 2009. Food and Agricultural Organization of the United Nations, 2009.

Hafizi, R., B. Salleh and Z. Latiffah. 2013. School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia. Brazilian Journal of Microbiology 44 (3): 959-968.

Haqqani, A. M., M. A. Zahid and M. R. Malik. 2000. Legumes in Pakistan. In: Legumes in rice and wheat cropping systems of the Indo-Gangetic Plainconstraints and opportunities (eds. International Crops Research Institute for the Semi-Arid Tropics, Cornell University, Ithaca, New York, USA. pp. 98-128.

Hatamleh, A.A., A.H. Bahkali, M. El-Sheshtawi, M.A. Elmetwally and A.M. Elgorban, 2014. Inhibitory influence of plant extracts on soil borne fungi infecting Muskmelon (Cucumis melo L.). International Journal of Pharmacology. 10: 322-327.

Hatamleh, A.A., A.H. Bahkali, M. El-Sheshtawi, M.A. Elmetwally and A.M. Elgorban, 2014. Inhibitory influence of plant extracts on soil borne fungi infecting Muskmelon (Cucumis melo L.). International Journal of Pharmacology. 10: 322-327.

Hur, J.S., S.Y. Ahn, Y.J. Koh and C.I. Lee, 2000. Antimicrobial properties of cold-tolerant eucalyptus species against phytopathogenic fungi and food-borne bacterial pathogens. The Plant Pathology Journal. 16: 286-289.

Ingels, C., M. VanHorn, R.L. Bugg, and P.R. Miller. 1994. Selecting the right cover crop gives multiple benefits. California Agriculture 48(5):43-48.

Joshi, M., R. Srivastava, A. K. Sharma and A. Prakash. 2013. Isolation and characterization of Fusarium oxysporum, a wilt causing fungus, for its pathogenic and non-pathogenic nature in tomato (Solanum lycopersicum). Journal of Applied and Natural Science, 5(1), pp.108-117.

Katooli, N., R. Maghsodlo and S.E. Razavi, 2011. Evaluation of eucalyptus essential oil against some plant pathogenic fungi. Journal of plant Breeding and Crop Sciences. 3: 41-43.

Khvostova, V.V. 1983. Breeding and Genetics of Peas. Oxonian Press Pvt. Ltd., New Delhi, India.

Mandhare, V. K., G. P. Deshmukh, J. V. Patil, A. A. Kale and U. D. Chavan. 2011. Mophological, pathogenic and molecular characterization of Fusarium oxysporum f. sp. cicero isolates from Maharashtra, India. Indonesian Journal of Agricultural Science. 12(2): 47-56.

Salleh, T. Yli-Mattila, K.R.N. Reddy and S. Abbasi. 2011. Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah province, Iran. Saudi Jpurnal of Biological Sciences 8(4): 341–351

Sardana S., R. K. Mahajan, N. K. Gautam and B. Ram. 2007. Genetic variability in pea (Pisum sativum L.) germplasm for utilization. SABRAO Journal of Animal Breeding and Genetics 39(1): 31-42

Smartt, J. 1990. Evolution of genetic resources. In: Grain Legumes. (Ed.): J. Smartt. Cambridge University Press, Cambridge, 140-145 pp

Stoilova, T. and P. Chavdarov. 2006. Evaluation of lentil germplasm for disease resistance to Fusarium wilt (Fusarium oxysporum f. sp. lentis). Journal of Centrel Eurpeon Agriculture. 7(1): 121-126.

Su, Y.C., C.L. Ho, E.I.C. Wang and S.T. Chang, 2006. Antifungal activities and chemical compositions of essential oils from leaves of four eucalypts. Taiwan Journal of Forest Science. 21: 49-61.

Timmerman-Vaughan G. M., A. Mills, C. Whitfield, T. Frew, R. Butler and S. Murray. 2005. Linkage mapping of QTL for seed yield, yield components, and developmental traits in pea. Crop Sciences 45: 1336–1344.

ÚKZÚZ, 2008. Field pea. In: Central Institute for Supervising and Testing in Agriculture, National plant variety office (ed.). Varieties. 145-160 PP.

Urbano G, P Aranda and E Gomez-Villalva, 2003. Nutritional evaluation of pea (Pisum sativum L.) protein diets after mild hydrothermal treatment and with and without added phytase. Journal of Agricultural and Food Chemistry. 51: 2415–2420.




DOI: https://doi.org/10.33866/10.33866/phytopathol.030.02.0534

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Asfand Iqbal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

      
   
Pakistan Journal of Phytopathological
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.