CHARACTERIZATION AND PATHOGENICITY OF FUSARIUM PROLIFERATUM ON DATE PALMS IN ALGERIA

Safia Sahouli, Jose Sanchez, Eduardo Gallego, Aminata O.E.H. khelil

Abstract


During a survey conducted from 2013-2014, in order to determine the fungal agents of wilt and dieback of date palms (Phoenix dactylifera L.) from Ghardaia province in Southern Algeria, two Fusarium species; Fusarium oxysporum, Fusarium proliferatum and Thielaviopsis sp were identified based on their cultural and morphological characteristics. Fusarium proliferatum (Matsush.) Nirenberg ex Gerlach & Nirenberg was isolated and identified, and Koch’s postulates were completed. Morphological identification was based on morphological characteristics on PDA and SNA culture media, and it was confirmed molecularly using ribosomal DNA sequence. Data comparison of the sequence (KR 856363) at GenBank revealed 100 % similarity to this fungal species. A phytopathogenicity test was carried out with 3 months old plants, and they were inoculated by dipping the roots into a fungal conidial suspension and also compared with Fusarium oxysporum f. sp. albedinis (Kill. & Maire) Malençon, known causal agent of date palm Fusarium wilt (Bayoud disease). After 90 days, disease incidence was eventually 76.66 % and 80 % for F. proliferatum and F. oxysporum, respectively, and the calculus of the index shows that this fungus would be a very serious threat to date palm. Microscopic observation of the roots provided confirmatory evidence that F proliferatum is causing a vascular disease on date palm.


Keywords


Phoenix dactylifera, Fusarium proliferatum, pathogenicity, Fusarium wilt, Microscopic observation.

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References


A. A. W. Aletby, M. 2016. First Record of Red Palm weevil, Rhynchophorus ferrugineus (Olivier, 1790) on the Date Palm, Phoenix dactylifera in Basrah, Iraq. Basrah Journal of Agricultural Sciences, 29: 1-6.

Abdalla, M. Y. 2001. Sudden Decline of Date Palm Trees Caused by Erwinia chrysanthemi. Plant Disease, 85: 24-26.

Abdalla, M. Y., A. Al-Rokibah, A. Moretti and G. Mulè. 2000. Pathogenicity of Toxigenic Fusarium proliferatum from Date Palm in Saudi Arabia. Plant Disease, 84: 321-324.

Abdullah, S.K., L.V Lopez Lorca., H.B Jansson.2010. Diseases of date palms (Phoenix dactylifera L.). Basrah J. Date Palm Res, 9:1–44.

Alwahshi, K., E. Saeed, A. Sham, A. Alblooshi, M. Alblooshi, K. El-Tarabily and S. AbuQamar. 2019. Molecular Identification and Disease Management of Date Palm Sudden Decline Syndrome in the United Arab Emirates. International Journal of Molecular Sciences, 20: 923.

Armengol, J., A. Moretti, G. Perrone, A. Vicent, J. A. Bengoechea and J. García-Jiménez. 2005. Identification, incidence and characterization of Fusarium proliferatumon ornamental palms in Spain. European Journal of Plant Pathology, 112: 123-131.

Bani, M., A. Pérez-De-Luque, D. Rubiales and N. Rispail. 2018. Physical and Chemical Barriers in Root Tissues Contribute to Quantitative Resistance to Fusarium oxysporum f. sp. pisi in Pea. Frontiers in Plant Science, 9.

Beckman, C. H. 1987. The nature of wilt diseases of plants. APS press.

Chao, C. T. and R. R. Krueger. 2007. The Date Palm (Phoenix dactylifera L.): Overview of Biology, Uses, and Cultivation. HortScience, 42: 1077-1082.

Djerbi, M. 1998. Diseases of the Date Palm: Present Status and Future Prospects. Journal of Agricultural and Marine Sciences [JAMS], 3: 103.

El Aoufir, A. 2002. Etude du fletrissement vasculaire du pois chiche (Cicer arietinum) cause par le Fusarium oxysporum f. sp. ciceri: Evaluation de la fiabilite de l'analyse isoenzymatique et de la compatibilite vegetative pour la caracterisation des races physiologiques (French text).

Gerlach, W. and H. Nirenberg. 1983. The Genus Fusarium: A Pictorial Atlas. Mycologia, 75: 1110.

Leslie, J. and B. Summerell. 2006. The fusarium laboratory manual. 2006. Ames, Iowa: Blackwell Publishing CrossRef Google Scholar, 388.

Lindner, D. L. and M. T. Banik. 2011. Intragenomic variation in the ITS rDNA region obscures phylogenetic relationships and inflates estimates of operational taxonomic units in genus Laetiporus. Mycologia, 103: 731-740.

Logrieco, A., B. Doko, A. Moretti, S. Frisullo and A. Visconti. 1998. Occurrence of Fumonisin B1and B2 in Fusarium proliferatumInfected Asparagus Plants. Journal of Agricultural and Food Chemistry, 46: 5201-5204.

Louvet,J., J.Bulit. 1972. Le Bayoud, fusariose vasculaire du palmier dattier : Symptômes et nature de la maladie. In : Le palmier dattier et sa fusariose vasculaire (Bayoud),DRA-Maroc, INRA-France.

Luongo, L., A. Ferrarini, A. Haegi, S. Vitale, A. Polverari and A. Belisario. 2014. Genetic Diversity and Pathogenicity of Fusarium oxysporumf.sp. melonisRaces from Different Areas of Italy. Journal of Phytopathology, 163: 73-83.

Mansoori, B. 2012. Fusarium proliferatuminduces gum in xylem vessels as the cause of date bunch fading in iran.

Mansoori, B. and M. H. Kord. 2006. Yellow Death: A Disease of Date Palm in Iran Caused by Fusarium solani. Journal of Phytopathology, 154: 125-127.

Masratul Hawa, M., B. Salleh and Z. Latiffah. 2013. Characterization and pathogenicity of Fusarium proliferatum causing stem rot of Hylocereus polyrhizusin Malaysia. Annals of Applied Biology, 163: 269-280.

Nelson, P. E., T. A. Toussoun and W. Marasas. 1983. Fusarium species: an illustrated manual for identification.

Oechsler, R. A., M. R. Feilmeier, D. R. Ledee, D. Miller, M. R. Diaz, M. E. Fini, J. W. Fell and E. C. Alfonso. 2009. Utility of Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusariumspp. from Ocular Sources. Investigative Opthalmology & Visual Science, 50: 2230.

Péreau-Leroy, P. and P. Pereau-Leroy. 1958. Le palmier dattier au Maroc. Institut français de recherches fruitiers outre-mer (IFAC) Paris.

Pietro, A. D., M. P. Madrid, Z. Caracuel, J. Delgado-Jarana and M. I. G. Roncero. 2003. Fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. Molecular Plant Pathology, 4: 315-325.

Saaidi, M. 1979. Contribution à la lutte contre le bayoud, fusariose vasculaire du palmier dattier.

Saleh, A. A., A. H. Sharafaddin, M. H. El_Komy, Y. E. Ibrahim, Y. K. Hamad and Y. Y. Molan. 2016. Fusarium species associated with date palm in Saudi Arabia. European Journal of Plant Pathology, 148: 367-377.

Sedra, M. H. 2010. Evaluation of soil receptivity of date palm groves in arab countries to Fusarium oxysporum f. sp. albedinis, causal agent of bayoud disease of date palm. Acta Horticulturae: 515-525.

Seem, R. C. 1984. Disease Incidence and Severity Relationships. Annual Review of Phytopathology, 22: 133-150.

Shahnazi, S., S. Meon, G. Vadamalai, K. Ahmad and N. Nejat. 2012. Morphological and molecular characterization of Fusarium spp. associated with yellowing disease of black pepper (Piper nigrum L.) in Malaysia. Journal of General Plant Pathology, 78: 160-169.

Stępień, Ł., G. Koczyk and A. Waśkiewicz. 2011. Genetic and phenotypic variation of Fusarium proliferatum isolates from different host species. Journal of Applied Genetics, 52: 487-496.

Tamburini, E., E. Mamolini, M. De Bastiani and M. Marchetti. 2016. Quantitative Determination of Fusarium proliferatum Concentration in Intact Garlic Cloves Using Near-Infrared Spectroscopy. Sensors, 16: 1099.

White, T. J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. PCR Protocols. Elsevier, pp. 315-322.

Zaid, A., P. de Wet, M. Djerbi and A. Oihabi. 2002. In Date palm cultivation, Diseases and pests of date palm, ed Zaid A. Food and Agriculture Organization Plant Production and Protection Paper: 227-281.




DOI: https://doi.org/10.33866/phytopathol.032.01.0546

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Pakistan Journal of Phytopathological
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