EFFECT OF SEED BIO PRIMING WITH RHIZOBACTERIA AGAINST ROOT ASSOCIATED PATHOGENIC FUNGI IN CHICKPEA

Muhammad Sufyan, Muhammad I. Tahir, Muhammad I. U. Haq, Shabir Hussain, Muhammad Saeed

Abstract


Chickpea (Cicer arietinum L.) among other pulse legumes contribute majorly in economy of a country. Yield and quality of the produce is seriously affected by various soil borne pathogens whereas now-a-days use of PGPR as a substitute to chemicals is very effective. Chickpea desi variety (Bittal-98) was primed by hydrobio priming and drum priming methods using two rhizobacterial isolates i.e. Pseudomonas putida and Pseudomonas fluorescens. Both methods showed effective disease control i.e. drum priming (27.16%) and hydro-biopriming (30.5%) and improved growth parameters. The maximum shoot length was observed in drum priming T3 (22.40cm) as compared to control (7.88cm). The root length also varied significantly and the result ranged from 3.78cm (Control) to 12.58cm (T3 Drum priming). Similarly plants fresh weight (0.91gm) and plants dry weight (0.63gm) also considerably enhanced by drum priming in comparison with control (0.43gm and 0.27gm, respectively). The most effective treatment was when both rhizobacteria were applied together i.e. T3 thus resonating the effect of each other.


Keywords


PGPR; biopriming; root fungi; chickpea

Full Text:

PDF

References


Bayraktar, H. and F. S. Dolar. 2009. Genetic Diversity of Wilt and Root Rot Pathogens of Chickpea, as Assessed by RAPD and ISSR. Turk. J. Agric. For., 33: 1-10.

Beneduzi, A., A. Ambrosini and L. M. P. Passaglia. 2012. Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents. Genetics & Mol. Biol., 35, 4: 1044-1051.

Bennett, A. J. and J. M. Whipps. 2008. Dual application of beneficial microorganisms to seed during drum priming. Applied soil ecology, 38: 83-89.

Caseiro, R., M. A. Bennett and J. Marcos-Filho. 2004. Comparison of three priming techniques for onion seed lots differing in initial seed quality. Seed Science and Technology, 32: 365-375.

Dobbelaere, S., A. Croonenborghs, A. Thys, D. Ptacek, Y. Okon and J. Vanderleyden. 2002. Effect of inoculation with wild type Azospirillum brasilense and A. irakense strains on development and nitrogen uptake of spring wheat and grain maize. Biology and Fertility of Soils, 36(4): 284-297.

Entesari, M., F. Sharifzadeh, M. Ahmadzadeh and M. Farhangfar. 2013. Seed Biopriming with Trichoderma Species and Pseudomonas fluorescent on Growth Parameters, Enzymes Activity and Nutritional Status of Soybean. Intl. J. Agron. Plant. Prod., 4 (4): 610-619.

Erdogan, O. and K. Benlioglu. 2010. Biological control of Verticillium wilt on cotton by the use of fluorescent Pseudomonas spp. Under field conditions.Biol control, 53: 39-45.

Frankenberger, J. R. W. T. and M. Arshad. 1995. Phytohormones in soils. Microbial Production and Function, Marcel Dekker, Inc. New York. pp. 5-40.

Gravel, V., H. Antoun and R. J. Tweddell. 2007. Growth stimulation and fruit yield improvement of greenhouse tomato plants by inoculation with Pseudomonas putidaor, Trichoderma atroviride: Possible role of indole acetic acid (IAA). Soil Biology & Biochemistry, 39:1968-1977.

Halmer, P. 2004. Methods to improve seed performance in the field. (Eds.), Handbook of Seed Physiology: Applications to Agriculture. In: Benech-Arnold, R. L and R. A. Sa´nchez. The Haworth Press Inc., New York, pp. 125-166.

Harris, D., B. S. Raghuwenshi, J. S. Gangwar, S. C. Singh, K. B. Joshi, A. Rashid and P. A. Hollington. 2001. Participatory evaluation by farmers of on-farm seed priming in wheat in India, Nepal and Pakistan. Exp. Agric., 37: 403-415.

Harris, D., B. S. Raghuwenshi, J. S. Gangwar, S. C. Singh, K. B. Joshi, A. Rashid and P. A. Hollington. 2001. Participatory evaluation by farmers of on-farm seed priming in wheat in India, Nepal and Pakistan. Exp. Agric., 37: 403-415.

Jukanti, A.K., Gaur, P.M., Gowda, C.L.L. and Chibbar, R.N. (2012). Nutritional quality and health benefits of chickpea(Cicer arietinum L.): A review. Br. J. Nutr. 108: 11-26

Kamal, A. M., Abo-Elyousr, H. Hoda and El-Hendawy. 2008. Integration of Pseudomonas fluorescens and acibenzolar-Smethyl to control bacterial spot disease of tomato. Crop Prot., 27: 1118-1124.

Kaur, R., J. Macleod, W. Foley and M. Nayudu. 2006. Gluconic acid: an antifungal agent produced by Pseudomonas species in biological control of take-all. Phytochemistry, 67 (6): 595-604.

Kumar, V., A. Kumar and R. N. Kharwar. 2007. Antagonistic potential of fluorescent pseudomonads and control of charcoal rot of Chickpea caused by Macrophomina phaseolina. J. Environ. Biol., 28(1): 15-20.

Mancini, V. and G. Romanazzi. 2013. Seed treatments to control seedborne fungalpathogens of vegetable crops. (wileyonlinelibrary.com) DOI 10.1002/ps.3693.

Maleki, M., S. Mostafaee, L. Mokhtarnejad and M. Farzaneh.2010. Characterization of Pseudomonas fluorescens strain CV6 isolated from cucumber rhizosphere inVaramin as a potential biocontrol agent. Aust. J. Crop Sci., 4(9): 676-683.

Moeinzadeh, A., F. S. Zadeh, M. Ahmadzadeh and F. H. Tajabadi. 2010. Biopriming of sunflower (Helianthus annuus L.) seed with Pseudomonas fluorescens for improvement of seed invigoration and seedling growth. Aust. J. Crop Sci., 4: 564 - 570.

Nakkeeran, S., W. G. Dilantha Fernando and Z. A. Siddiqui. 2005. Plant Growth Promoting Rhizobacteria Formulations and Its Scope In Commercialization For The Management Of Pests And Diseases. Biocontrol and Biofertilization, 257-296.

Pakistan Bureau of Statistics, Provisional 2018-2019. (Available on http://www.finance.gov.pk/survey_1819.html).

Pande, S., J. NarayanaRao and M. Sharma. 2007. Establishment of the chickpea wilt pathogen Fusarium oxysporum f. sp. ciceris in the soil through seed transmission. Plant Pathol. J. 23(1): 3-6.

Park, C. S., O. Choi, J. Kim and Choong-Min, Ryu. 2004. Colonization and Popultion Changes of a Biocontrol Agent, Paenibacillus polymyxa E681, in Seeds and Roots.

Rai, A. K. and A. K. Basu. 2014. Pre-Sowing Seed Bio-Priming in Okra: Response for Seed Production. The Bioscan. 9(2): 643-647.

Raj, S. N., N. P. Shetty and H. S. Shetty. 2004. Seed bio-priming with Pseudomonas fluorescens isolates enhances growth of pearl millet plants and induces resistance against downy mildew. Int. J. Pest Manage.,50 (1): 41-48.

Rashid, D. and B. Ahmed. 2005. Effect of iron and growth inhibitors on siderophores production by Pseudomonas fluorescens. Afr. J. Biotechnol., 4 (7): 697-702.

Rangaswami, G. 1972. Diseases of Crop Plants in India, Prentice Hall of India Pvt. Ltd., New Delhi.p. 520.

Shaukat, K., S. A. Frasayab and S. Hasnain. 2006. Growth responses of Helianthus annus to plant growth promoting rhizobacteria used as a biofertilizer. Journal of Agricultural Research, 1(6):573-581.

Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedures of Statistics: a biometrical approach. McGraw-Hill Publishing co. Inc. New York. p. 633.

Suzuki, S., Y.He and H. Oyaizu. 2003. Indole-3-acetic acid production in Pseudomonas fluorescens HP72 and its association with suppression of creeping bent grass brown patch. Journal Current Microbiology, 47(2): 138-143.

Suslow TV, Schroth MN, Isaka M, 1982. Application of a rapid method for gram differentiation of plant pathogenic and saprophytic bacteria without staining. Phytopathology, 72: 917-918.

Schaad NW, 1980. Laboratory guide for the identification of plant pathogenic bacteria. American Phytopathological Society. Saint Paul. Minnesota. pp. 28-45.

Tahir, M.I., M. I. Haq, M. Ashfaq, N. A. Abbasi, H. Butt, H. Ghazal. 2016. Screening of effective antagonists from potato rhizosphere against bacterial wilt pathogen. Intl J. Biosci., 8(2): 228-240.

Venkatasubramanian, A. and R. Umarani. 2007. Evaluation of seed priming methods to improve seed performance of tomato (Lycoperison esculentum), eggplant (Solanum melongena) and chilli (Capsicum annum). Seed Sci. Technol., 35: 487-493.

Weller, D. M. and R. Cook. 1996. Increased growth of chickpea by seed treatment with Fluorescent pseudomonads and implications of pythium control. Can. J. Plant Pathol., 8: 328-344..

Wollum, A. G. 1982. Methods of soil analysis.Medison Wisconsin, USA. pp. 781-801.




DOI: https://doi.org/10.33866/phytopathol.032.01.0567

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Muhammad Ibrahim Tahir, Muhammad Sufyan, Muhammad Inam ul Haq, Shabir Hussain, Muhammad Saeed

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

      
   
Pakistan Journal of Phytopathological
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.