Abdul R. Malik, Shafqat Ali, Muhammad N. Khan, Salman Ahmad, Muhmmad A. Ali


Citrus canker is one of the most devastating disease of citrus crop. It is a worldwide disease, caused by bacterium Xanthomonas axonopodis pv. Citri. Citrus canker incidence has been observed on most of the citrus varieties. Severe infection of the bacterium produces a variety of effects including defoliation, dieback, severely blemished fruit, reduced fruit quality and premature fruit drop. Present study was conducted at Citrus Research Institute, Sargodha during the years 2016-18 on ten years old kinnow plants. There were ten treatments including control with four replications. Spray was done four times in a year during the months of March, April, August, and September. The efficacy of different treatments was compared to control the disease incidence on leaves and fruits. It was concluded that bordeaux mixture was the most suitable chemical to control the canker on kinnow plant and it also improved the quality of fruit in terms of fruit weight, fruit size and juice weight. Besides, copper hydroxide and onion extract were also found efficacious to control the disease. In future, these chemicals and plant extracts could be used in different combinations to control canker on different citrus varieties.


Fungicides; Botanical extracts; Citrus canker; Xanthomonas axonopodis pv. Citri; kinnow

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Ali, M. R., M. F. Hasan, R. S. Lia, A. Akhter, M. S. E. Sumi, M. F. Hossain, M. Islam, M. Khalekuzzaman and B. Sikdar. 2017. Isolation and characterization of a canker disease causing pathogen from Citrus aurantifolia and evaluation of its biological control measure. Journal of Entomology and Zoology Studies, 5: 1526-1532.

Anonymous. 2017. Crop reporting services. Govt of Punjab, Pakistan.

Arif, A.G., M. Akhtar and M. Ibrahim. 1964. Citrus disease and their control. Punjab Fruit Journal, 26: 27369-70.

Atiq, M., M. A. Khan and S. Sahi. 2007. Screening of citrus germplasm for the sources of resistance against canker disease caused by Xanthomonas axonopodis pv. Citri. Pakistan Journal of Phytopathology, 19: 222-226.

Atiq, M., M. Aslam Khan, S. Talib Sahi, R. Ahmad, M. Younas, M. Shafiq and Y. Ali. 2018. Appraisal of plant extracts and streptomycin sulfate against citrus canker disease. Archives of Phytopathology and Plant Protection, 51: 824-833.

Balaraman, K. And R. Purushotman. 1981. Control of citrus canker on acid lime. South Indian Horticulture, 29: 175-177.

Beniwal, S. And B. SPS. 1976. Thiram, a fungicide effective against Xanthomonas citri (hasse) dowson. Pesticides, 10:31-36.

Britto, S. John, and S. Senthilkumar. 2001. Antibacterial activity of Solanum incanum L. leaf extracts. Asian Journal of Microbiology, Biotechnology & Environmental Sciences 3: 65-66.

Bylka, W., I. Matlawska and N. Pilewski. 2004. Natural flavonoids as antimicrobial agents. Jana, 7: 24-31.

Chakravarti, B. And S. Hegde. 1970. Studies on citrus canker in Rajasthan. III. Control of citrus canker by antibiotics and chemicals. Punjab Horticultural Journal, 10: 161-165.

Chand, J. And V. Pal. 1982. Citrus canker in India and its management. Problems of citrus diseases in India/edited by SP Raychaudhuri, YS Ahlawat. Surabhi printers and publishers, New Delhi. PP. 21-26.

Civerolo, E. L. "Citrus bacterial canker disease: An overview." Proceedings of the International Society of Citriculture/[International Citrus Congress, November 9-12, 1981, Tokyo, Japan; K. Matsumoto, editor]. Shimizu, Japan: International Society of Citriculture, 1982-1983., 1982.

Das, A. 2003. Citrus canker-A review. Journal of Applied Horticulture, 5: 52-60.

Dorman, H. D. And S. G. Deans. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of applied microbiology, 88: 308-316.

Folico - de – Olaroz, L. 1986. Multiplication of Xanthomonas competitive.Citri in leaf tissue of different citrus species. Fitopatologia, 21: 52-60.

Garnsey, S. M., E. L. Clichorme, J. W. Field, C. P. Seymour, J. T. Griffiths. 1979. Citrus Canker. Citrus Indus, 60: 5-6.

Graham, J., T. Gottwald, E. Civerolo and R. Mcguire. 1989. Population dynamics and survival of Xanthomonas campestris in soil in citrus nurseries in Maryland and Argentina. Plant Disease, 43: 423-427.

Hostettmann, Kurt, and Jean‐Luc Wolfender. 1997. The search for biologically active secondary metabolites. Pesticide science 51: 471-482.

Khan, W. A., M. Atiq, S. T. Sahi, A. A. Khan, S. Ali, M. Younas, Y. Ali, M. R. Bashir and M. Sajid. 2018. Induction of Resistance against Citrus Canker through Chemicals and Plant Activators. Advances in Zoology and Botany, 6: 26-30.

Kilani, A. 2006. Antibacterial assessment of whole stem bark of Vitex doniana against some enterobactriaceae. African Journal of Biotechnology, 5.

Kishun, R. and L. Chand. 1987. Studies on germplasm resistance and chemical control of citrus canker. Indian Journal of Horticulture, 44:126-32.

Krishna, Ashok, and A. G. Nema. 1983. Evaluation of chemicals for the control of citrus canker. Indian Phytopathology, 36: 348-50.

Mahajan, A. And S. Das. 2003. Plants and microbes-Potential source of pesticide for future use. Pesticides information, 28: 33-38.

Parameswari, C. And A. Tulasi latha. 2001. Antibacterial activity of Ricinus communis leaf extract. Indian drugs, 38: 587-588.

Patel, M. And A. Padhya. 1964. Sodium arsenite-Copper sulphate spray for the control of citrus canker. Current Science, 33: 87-88.

Rath, C., S. Dash and R. Mishra. 2001. In vitro susceptibility of Japanese mint (Mentha arvensis L.) Essential oil against five human pathogens. Indian Perfumer, 45: 57-62.

Rehman, M., N. Chaudhary and M. Nasir. 2006. Citrus canker and its control through chemicals and antibiotics under Sargodha climatic conditions. Pakistan Journal of Phytopathology, 18: 67-69.

Rossetti, V. 1977. Citrus canker in Latin America: A review. Proceedings of the International Society of Citriculture. Pp. 918-923.

Sahi, S. T., M.U. Ghazanfar ,M. Afzal, A. Rashed, A. Habib. 2007. Incidence of citrus canker diseases caused by Xanathomonas campestris pv.Citri (Hasse) Down on Kinnow (Citrus Reticulata) and its chemotherapy. Pakistan Journal of Botany, 39: 1319-1327.

Saleem, A. and A. H. Tariq. 1992. Important disease and their control in Punjab. Proceedings of 1st International Seminar. Citriculture in Pakistan. pp. 293-98.

Sattar, A. And A. Hafiz. 1952. Research on plant diseases of the Punjab.

Shimpi, S. And R. Bendre. 2005. Stability and antibacterial activity of aqueous extracts of Ocimum canum leaves. Indian perfumer, 49: 225.

Stall, R. E. And C. P. Seymour. 1983. Canker, a threat to citrus in the Gulf-Coast states. Plant Disease, 67: 581-585.

Sundin, G. W., D. H. Demezas and C. L. Bender. 1994. Genetic and plasmid diversity within natural populations of Pseudomonas syringae with various exposures to copper and streptomycin bactericides. Applied and Environmental Microbiology, 60: 4421-4431.

Tahir, H. A., S. T. Sahi, A. Habib, I. U. Haq, A. Ahmad and W. Ashraf. 2016. Evaluation of plant extracts as biocontrol agents against Xanthomonas axonopodis pv citri the cause of citrus canker. Pakistan Journal of Phytopathology, 28: 35-43.

Thakre, B., U. Soni, C. Gour and S. Dharpure. 2017. Effect of different chemicals for the control of citrus canker caused by Xanthomonas axonopodis pv. Citri. Plant Archives, 17: 1181-1183.

White, D. G., S. Zhao, S. Simjee, D. D. Wagner and P. F. Mcdermott. 2002. Antimicrobial resistance of foodborne pathogens. Microbes and infection, 4: 405-412.

Williams, R. J. And D. L. Heymann. 1998. Containment of antibiotic resistance. American Association for the Advancement of Science. 279: 1153-1154.

Yesmin, K., M. Ahmad, M. Momtaz and K. Begum. 2017. Effect of fungicides and plant extracts in the management of foliar, twig and fruit diseases of citrus (Citrus limon). Journal of Environmental Science and Natural Resources, 10: 93-100.



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