Amjad Ali, Muhammad A. Zeshan, Yasir Iftikhar, Muhammad Abid, Syeda F. Ehsan, Muhammad U. Ghani, Awais A. Khan


Chilli (Capsicum annuum), cultivated as spicy crop and has played a vital role in the maintenance of human body. Chilli veinal mottle virus (ChiVMV) is one of the most destructive and fast spreadable disease by vector and mechanically worldwide. The present study confirms the mechanical inoculation of ChiVMV in host plant by leaf inoculation method. Reducing agent carbon tetrachloride (CCl4) and chloroform (CHCl) with ratio 5% and 2.5% reduced the infectivity and longevity of crude virus sap by mixing with them. The exogenous application of chemical inducer (Salicylic acid SA) and botanicals (Eucalyptus and Mint) reduced the disease incidence and severity of ChiVMV in greenhouse study and these were ecofriendly in nature. Salicylic Acid decreased severity (28%) followed by Eucalyptus (11.05%) and Mint (9.66%). The final chlorophyll contents study of healthy and diseased leaf was done with spectrophotometer by using different wavelength absorbance (645nm, 663nm, 652nm and 490nm), the healthy leaf of chilli plant shows more a, b and total chlorophyll as compared to diseased leaves.


Mechanical inoculation; Botanicals; Chemical inducers; Salicylic Acid; Spectrophotometer

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