CONSERVATION OF CEREALFUNGI FOLLOWING DIFFERENT METHODS OF PRESERVATION FOR LONG TERMS

Nachaat Sakr

Abstract


Preservation of soil- and air-borne phytopathogenic fungi for extent durations of time is crucial so that analyses can be followed up any moment. The goal of the present research was to analyze storage methodologies in maintaining field isolates of Fusarium head blight (FHB), spot blotch (SB) and common root rot (CRR) diseases. Cereal fungal isolates were stored at four independent storage experiments (1) filter paper disks carrying fungal mycelium at 4°C, (2) fungal suspension at ambient temperature, (3) fungal suspension at 4°C and (4) mycelial cultures on Petri-dishes with potato dextrose agar by freezing at -16°C. The survival, absence of microbial contamination and morphological constancy of cultures were tested in periods ranging from 1 to 60 months. The isolates were recovered from the four preservation treatments having +% survival and maintaining their purity and morphological stability at each time point. Neither used storage technique, time in storage nor taxonomic classification was associated with a lack of viability, microbial contamination or morphology alternation. As far as we know, the present work is the first report highlighting the possibility of conservation for FHB, SB and CRR causal agents using filter paper disks stored in cold water for three years, fungal suspension at ambient temperature for 3 years, fungal suspension at 4°C in cold water and mycelial cultures by freezing for 5 years.


Keywords


cereal fungi, freezing, viability, water storage

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Abd-Elsalam, K. A., Yassin, M. A., Moslem, M. A., Bahkali, A. H., Mckenzie, E. H. C., Stephenson, S. L., Cai, L. and K. D. Hyde. 2010. Culture collections, the new herbaria for fungal pathogens. Fungal Divers. 45: 21–32.

Agrios, G. N. 2004. Plant pathology. Elsevier Academic, NJ. USA.

Arabi, M. I. E., Jawhar, M. and A. Al-Daoude. 2007. Viability of Cochliobolus sativus cultures after storage under different conditions. J. Plant Pathol. 89: 79-83.

Baskarathevan, J., Jaspers, M. V., Jones, E. E. and H. J. Ridgway. 2009. Evaluation of different storage methods for rapid and cost-effective preservation of Botryosphaeria species. New Zealand Plant Prot. 62: 234-237.

Borman, A. M., Szekely, A., Campbell, C. K. and E. M. Johnson. 2006. Evaluation of the viability of pathogenic filamentous fungi after prolonged storage in sterile water and review of recent published studies on storage methods. Mycopathologia 161: 361–368.

Bueno, C. J., Ambrosio, M. M. De. Q. and N. L. De. Souza. 2006. Storage of soilborne phytopathogenic fungi. Summa Phytopathol. 32: 42-50.

Bunse, T. and G. K. Steigleder. 1991. The preservation of fungal cultures by lyophilization. Mycoses, 34: 173-176.

De Capriles, C. H., Mata, S. and M. Middelveen. 1989. Preservation of fungi in water (Castellani): 20 years. Mycopathologia 106: 73-79.

Dhingra O. D. and J. B. Sinclair 1985. Basic Plant Pathology Methods. CRC Press, FL. USA

Diogo, H. C., Sarpieri, A. and M. C. Pires. 2005. Fungi preservation in distilled water. An. Bras. Dermatol. 80: 591-594.

Elliott, M. L. 2005. Survival, growth and pathogenicity of Gaeumannomyces graminis var. graminis with different methods of long term storage. Mycologia 97: 901–907.

Guimaraes, L. C., Fernandes, A. P., Chalfoun, S.M. and L. R. Batista. 2014. Methods to preserve potentially toxigenic fungi. Braz. J. Microbiol. 4: 43-47.

Holden, A. N. G. and D. Smith. 1992. Effects of cryopreservation methods in liquid nitrogen on viability of Puccina abrupta var. partheniicola urediniospores. Mycol. Res. 96: 473-476.

Hornby, D. and G. L. Bateman. 1998. Take-all Disease of Cereals: A Regional Perspective. CAB International, Wallingford.

Legard, D. E. and C. K. Chandler. 2000. Cryopreservation of strawberry pathogen in mechanical ultra-low temperature freezer. HortScience 35: 1357.

McGinnis, M. R., Padhye, A. A. and L. Ajello. 1974. Storage of stock cultures of filamentous fungi, yeasts, and some aerobic Actinomycetes in sterile distilled water. Appl. Microbiol. 28: 218–222.

Milosevic, M. B., Medic-Pap, S. S., Ignjatov, M. V. and D.N. Petrovic, 2007. Lyophilization as a method for pathogens long term preservation. Proc. Nat. Sci. Matica. Srpska. Novi. Sad. 113: 203-210.

Nakasone, K. K., Peterson, S. W. and S.C. Jong. 2004. Preservation and distribution of fungal cultures. In: Muller, G.M. (eds), Biodiversity of Fungi. Elsevier Academic Press, San Diego, pp: 3-11.

Roy, C. B., Srinivas, P. and C. K. Jacob. 2014. Relative efficacy of long-term storage methods on survival and virulence of Corynespora cassiicola and Phytophthora meadii pathogenic on rubber (Hevea brasiliensis). Rubber Sci. 27: 202-214.

Ryan, M. J., Smith, D. and P. Jeffries. 2000. A decision-based key to determine the most appropriate protocol for the preservation of fungi. World J. Microbiol. Biotechnol. 16: 183-186.

Sakr, N. 2019. Long term storage for five important cereal phytopathogenic species. Pak. J. Phytopatho. 31: 155-162.

Smith, D. and A. H. S. Onions. 1983. The Preservation and Maintaintenance of Living Fungi. Commonwealth Mycological Institute, Kew.

Windels, C. E., Burnes, P. M. and T. Kommedahll. 1993. Fusarium species stored on silica gel and soil for ten years. Mycologia 85: 21–23.




DOI: https://doi.org/10.33866/phytopathol.030.02.0584

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Pakistan Journal of Phytopathological
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