RESISTANCE DURABILITY OF SEVERAL RICE CULTIVARS AGAINST RICE TUNGRO DISEASE IN SOUTH SULAWESI, INDONESIA

Nur Rosida, Tutik Kuswinanti, Nur Amin, Andi Nasruddin

Abstract


Tungro disease is one of the main obstacles to rice production in South and Southeast Asia. The cultivation of green leafhopper resistant rice and tungro virus-resistant rice is one of the ways to prevent tungro disease and has been successfully used to control the disease in the tropics. However, some of these varieties have been reported to have lost their resistance due to the adaptation of resistance in green leafhopper population against tungro resistant varieties. Until now, rice varieties that are resistant to green leafhoppers and rice tungro virus which are still frequently used by farmers in South Sulawesi are IR 64, Ciliwung, Inpari 9 Elo, and Inpari 7 Lanrang varieties. Thus, it is necessary to evaluate the durability of these varieties, whether they are still resistant to tungro disease or not. Variety resistance was evaluated by calculating the reduction in plant height (%), percentage of rice tungro virus infection (%) and detection of the presence of rice tungro virus using a PCR analysis for RTBV and an RT-PCR for RTSV after being inoculated with tungro virus isolates from South Sulawesi. Results showed that all resistant varieties were infected by tungro bacilliform virus (RTBV): 100, 100, 80, and 80% infections on IR-46, Ciliwung, IR-64, and IR-66, respectively. For the RTV-resistant cultivars, only Inpari 9 Elo and Inpari 7 Lanrang were not infected by rice tungro spherical virus (RTSV). Therefore, it is concluded that to prevent transmission of the tungro virus, Inpari 9 Elo and Inpari 7 Lanrang can still be recommended for planting in South Sulawesi.


Full Text:

PDF

References


Angeles, E. and G. Khush. 1999. 38. A new gene for resistance to green leafhopper, Nephotettix virescens (Distant) in rice.

Angeles, E. and G. Khush. 2000. Genetic analysis of resistance to green leafhopper, Nephotettix virescens (Distant), in three varieties of rice. Plant breeding, 119: 446-448.

Athwal, D., M. Pathak, E. Bacalangco and C. Pura. 1971. Genetics of resistance to brown planthoppers and green leafhoppers in Oryza sativa L. 1. Crop Science, 11: 747-750.

Azzam, O. and T. C. Chancellor. 2002. The biology, epidemiology, and management of rice tungro disease in Asia. Plant Disease, 86: 88-100.

Azzam, O., M. Arboleda, K. Umadhay, J. De los Reyes, F. Cruz, A. Mackenzie and K. McNally. 2000. Genetic composition and complexity of virus populations at tungro-endemic and outbreak rice sites. Archives of virology, 145: 2643-2657.

Azzam, O., T. Imbe, R. Ikeda, P. Nath and E. Coloquio. 2001. Inheritance of resistance to rice tungro spherical virus in a near-isogenic line derived from Utri Merah and in rice cultivar TKM6. Euphytica, 122: 91-97.

Budot, B. O., J. R. Encabo, I. D. V. Ambita, G. A. Atienza-Grande, K. Satoh, H. Kondoh, V. J. Ulat, R. Mauleon, S. Kikuchi and I.-R. Choi. 2014. Suppression of cell wall-related genes associated with stunting of Oryza glaberrima infected with Rice tungro spherical virus. Frontiers in microbiology, 5: 1-9.

Bunawan, H., L. Dusik, S. N. Bunawan and N. M. Amin. 2014. Rice tungro disease: From identification to disease control. World Applied Sciences Journal, 31: 1221-1226.

Cabauatan, P. and H. Hibino. 1988. Isolation, purification, and serology of rice tungro bacilliform and rice tungro spherical viruses. Plant Disease, 72: 526-528.

Cabauatan, P., R. Cabunagan and H. Koganezawa. 1995. Biological variants of rice tungro viruses in the Philippines. Phytopathology, 85: 77-81.

Calleja, D. O. 2010. Water shortage due to El Nino breeds ‘tungro’ in rice plantations. Retrieved from http://balita.ph/2010/02/17/tungro-rice-disease-alert-up-in-bicol.

Choi, R. 2004. Current status of rice tungro disease research and future program. Strategi Pengendalian Penyakit Tungro: Status dan Program. Prosiding Seminar Nasional Status Program Penelitian Tungro Mendukung Keberlanjutan Produksi Padi Nasional. Makassar. pp. 7-8.

Chong, J., S. Yee and L. Eng. 2015. Rice Tungro Disease in Sarawak: past and present status. Pakistan Journal of Biological Sciences, 18: 285-289.

Doyl, J. and J. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 13-15.

Encabo, J. R., P. Q. Cabauatan, R. C. Cabunagan, K. Satoh, J.-H. Lee, D.-Y. Kwak, T. B. De Leon, R. J. A. Macalalad, H. Kondoh and S. Kikuchi. 2009. Suppression of two tungro viruses in rice by separable traits originating from cultivar Utri Merah. Molecular plant-microbe interactions, 22: 1268-1281.

Hibino, H. 1996. Biology and epidemiology of rice viruses. Annual review of phytopathology, 34: 249-274.

Hibino, H., M. Roechan and S. Sudarisman. 1978. Association of two types of virus particles with penyakit habang (tungro disease) of rice in Indonesia. Phytopathology, 68: 1412-1416.

Hull, R. 1996. Molecular biology of rice tungro viruses. Annual review of phytopathology, 34: 275-297.

Karim, A. R. and M. Pathak. 1982. New genes for resistance to green leafhopper, Nephotettix virescens (Distant) in rice, Oryza sativa L. Crop Protection, 1: 483-490.

Karim, A. R. and M. Pathak. 1982. New genes for resistance to green leafhopper, Nephotettix virescens (Distant) in rice, Oryza sativa L. Crop Protection, 1: 483-490.

Khwabe, S. K. 1985. Mechanism of varietal resistance to the rice green leafhopper (Nephotettix cincticeps Uhler). Jarq, 19: 115–124.

McCouch, S., L. Sebastian, R. Ikeda, N. Huang, T. Imbe and W. Coffman. 1996. Molecular mapping of resistance to rice tungro spherical virus and green leafhopper. Phytopathology, 86: 25-30.

McCouch, S., L. Sebastian, R. Ikeda, N. Huang, T. Imbe and W. Coffman. 1996. Molecular mapping of resistance to rice tungro spherical virus and green leafhopper. Phytopathology, 86: 25-30.

Nobuya, K., R. Ikeda and D. A. Vaughan. 1993. Resistance to rice tungro viruses in wild species of rice (Oryza spp.). Japanese Journal of Breeding, 43: 247-255.

Sama, S., A. Hasanuddin, I. Manwan, R. Cabunagan and H. Hibino. 1991. Integrated management of rice tungro disease in South Sulawesi, Indonesia. Crop Protection, 10: 34-40.

Satoh, K., H. Kondoh, T. B. De Leon, R. J. A. Macalalad, R. C. Cabunagan, P. Q. Cabauatan, R. Mauleon, S. Kikuchi and I.-R. Choi. 2013. Gene expression responses to Rice tungro spherical virus in susceptible and resistant near-isogenic rice plants. Virus research, 171: 111-120.

Sebastian, L., R. Ikeda, N. Huang, T. Imbe, W. Coffman, M. Yano, T. Sasaki and S. McCouch. 1996. Genetic mapping of resistance to rice tungro spherical virus (RTSV) and green leafhopper (GLH) in ARC11554, Rice Genetics III: (In 2 Parts). World Scientific, pp. 560-564.

Siwi, B. H. and G. S. Khush. 1977. New Genes for Resistance to the Green Leafhopper in Rice 1. Crop Science, 17: 17-20.

Suprihanto, S., E. Nurhayati and J. Harjosudarmo. 2013. Virulensi Isolat Rice tungro virus dari Beberapa Daerah Endemis Tungro di Indonesia. Jurnal Fitopatologi Indonesia, 9: 29.

Wahab, M. I., Satoto, R. Rachmat, A. Guswara and Suhana. 2017. Deskripsi varietas unggul baru padi. Badan Penelitian dan Pengembangan Pertanian. Kementerian Pertanian. pp 87.

Widiarta, I. N., D. Kusdiaman, S. S. Siwi and A. Hasanuddin. 2017. Varian efikasi penularan tungro oleh koloni-koloni wereng hijau Nephotettix virescens distant. Jurnal Entomologi Indonesia, 1: 50.




DOI: https://doi.org/10.33866/phytopathol.030.02.0594

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Nur Rosida

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

      
   
Pakistan Journal of Phytopathological
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.