Yaseen Khan, Amir Sohail, Tabassum Yaseen, Khushnood U. Rehman, Muhammad Noor, Kamran Akbar


Arbuscular mycorrhizal fungi (AMF) is one of the important micro-bionta, which has a huge influence on plant nutrients. In the past, the application of poultry litter has been well studied in various crops such as wheat, maize and sugarcane. However, the poultry litter under AMF has been poorly studied in some crops including lens Esculenta. In order to investigate the effect of AMF with poultry litter on lens Esculenta, an experiment was conducted in the year of 2020-2021 in Bacha Khan University, Pakistan. AMF increased plant biomass, as well as biochemical and microbial status, according to the results. The control parameters were compared to the treated plant under all conditions i.e treatment (T1) and treatment (T2), where treated plants, the Lens esculenta s show the maximum growth. The plant height in T1 is 16.40% with compared to T2 is 17.40%. 2) No of leaves in T1 is 10.16% with compared to T2 44.8%. The leaf area in T1 is 0.4952% with compared to T2 is 0.6018%. The ratio of chlorophyll a content in T1 is 0.112 with compared to T2 is 0.275, the ratio of chlorophyll b in T1 is 0.341 with compared to T2 0.538. T1 has a fresh shoot weight of 12.35gm compared to T2's 27.79gm, while T1 has a dry shoot weight of 2.99gm compared to T2's 6.3gm. The weight of fresh root in T1 is 0.81gm vs 1.01gm in T2, while the weight of dried root in T1 is 0.28gm vs 0.34gm in T2. These results indicates the response of AMF was high with poultry littler, this indicates the AMF could increase the Lens esculenta’s growth and productivity under poultry littler.


Arbuscular mycorrhizal fungi, Lens esculenta, Growth, yield, Poultry litter

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Blum, A. 1996. Crop responses to drought and the interpretation of adaptation. Drought Tolerance in Higher Plants: Genetical, Physiological and Molecular Biological Analysis. Springer Netherlands, pp. 57-70.

Bolan, N. S., A. A. Szogi, T. Chuasavathi, B. Seshadri, M. J. Rothrock and P. Panneerselvam. 2010. Uses and management of poultry litter. World's Poultry Science Journal, 66: 673-698.

Chinivasagam, H. N., M. Redding, G. Runge and P. J. Blackall. 2010. Presence and incidence of food-borne pathogens in Australian chicken litter. British Poultry Science, 51: 311-318.

Finlay, R. D. 2008. Ecological aspects of mycorrhizal symbiosis: with special emphasis on the functional diversity of interactions involving the extraradical mycelium. Journal of Experimental Botany, 59: 1115-1126.

Giovannetti, M. and B. Mosse. 1980. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist, 84: 489-500.

Holm, G. 1954. Chlorophyll Mutations in Barley. Acta Agriculturae Scandinavica, 4: 457-471.

Samanhudi, S., A. Yunus, B. Pujiasmanto and M. Rahayu. 2014. Effect of Organic Manure and Arbuscular Mycorrhizal Fungi on Growth and Yield of Young Ginger (Zingiber officinale Rosc.). IOSR Journal of Agriculture and Veterinary Science, 7: 01-05.

Hussain, F. 1989. Field and laboratory manual of plant ecology. National Academy of Higher Education, University Grants Commission.

Khan, M. A., K. B. Marwat, A. Amin, A. Nawaz, R. Khan, H. Khan and H. U. Shah. 2012. Soil Solarization: An Organic Weed-Management Approach in Cauliflower. Communications in Soil Science and Plant Analysis, 43: 1847-1860.

Khan, Y., X. Yang, X. Zhang, T. Yaseen, L. Shi and T. Zhang. 2020. Arbuscular mycorrhizal fungi promote plant growth of Leymus chinensis (Trin.) Tzvelev by increasing the metabolomics activity under nitrogen addition. Grassland Science, 67: 128-138.

Moore, P., T. Daniel, A. Sharpley and C. Wood. 1995. Poultry manure management: Environmentally sound options. Journal of soil and water conservation, 50: 321-327.

Ngodigha, E. and O. Owen. 2009. Evaluation of the bacteriological characteristics of poultry litter as feedstuff for cattle. Scientific Research and Essays, 4: 188-190.

Pawaar, J. S. and U. B. Kakde. 2012. Study of Arbuscular Mycorrhiza associated with some important medicinal plants in suburban area of Mumbai. Online International Interdisciplinary Research Journal, 2: 116-127.

Rashid, M., M. Ali, M. Bakr and A. Sarker. 2007. Botrytis gray mold: a new foliar disease of lentil in Bangladesh. Bangladesh Journal of Plant Pathology, 23: 93-94.

Richardson, A. D., S. P. Duigan and G. P. Berlyn. 2002. An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytologist, 153: 185-194.

Shortall, J. G. and W. C. Liebhardt. 1975. Yield and Growth of Corn as Affected by Poultry Manure. Journal of Environmental Quality, 4: 186-191.

Shukla, A., A. Kumar, A. Jha, Ajit and D. V. K. N. Rao. 2011. Phosphorus threshold for arbuscular mycorrhizal colonization of crops and tree seedlings. Biology and Fertility of Soils, 48: 109-116.

Sim, E. Y. S. and T. Y. Wu. 2010. The potential reuse of biodegradable municipal solid wastes (MSW) as feedstocks in vermicomposting. Journal of the Science of Food and Agriculture, 90: 2153-2162.

Sims, J. T. and D. C. Wolf. 1994. Poultry Waste Management: Agricultural and Environmental Issues. Advances in Agronomy Volume 52. Elsevier, pp. 1-83.

Singh, M. 2006. Response of rice (Oryza sativa) to biofertilizers in combination with fym and nitrogen. Journal of Ecobiology, 18: 363.

Song, Y. Y., R. S. Zeng, J. F. Xu, J. Li, X. Shen and W. G. Yihdego. 2010. Interplant Communication of Tomato Plants through Underground Common Mycorrhizal Networks. PLoS ONE, 5: e13324.

Wilkinson, K. G., E. Tee, R. B. Tomkins, G. Hepworth and R. Premier. 2011. Effect of heating and aging of poultry litter on the persistence of enteric bacteria. Poultry Science, 90: 10-18.

Yaseen, T. 2016. Arbuscular Mycorrhizal Fungi spores diversity and AMF infection in some medicinal plants of District Charsadda KPK. Pure and Applied Biology, 4: 1176-1182.



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Pakistan Journal of Phytopathology
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