IN VITRO ANTAGONISTIC EFFECTS OF TRICHODERMA HARZIANUM AGAINST WHEAT-BORNE FUNGAL PATHOGENS

Ayesha Bibi, Najam U.S Afshan, Fouzia Yaqoub

Abstract


Biological control of fungi is a potential alternative to the use of chemical pesticides, which have been proved to be harmful to the environment. Pathogenic fungi were isolated and identified from wheat on PDA medium. Trichoderma harzianum have been isolated from soil and found to be effective biocontrol agent against various wheat-borne pathogenic fungi. Trichoderma harzianum inhibited the growth of wheat-borne fungi like Aspergillus, Alternaria triticina, Fusraium solani, Rhizopus oryzae, Rhizactonia solani, Sclerotium rolfsii and Bipolaris in dual culture plate assay.


Keywords


Biological control, Wheat-borne pathogens and Trichoderma harzianum

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Ambuse, M., V. Chatage and U. Bhale. 2012. Influence of Trichoderma spp. against Alternaria tenuissima inciting leaf spot of Rumex acetosa L. Bioscience Discovery, 3: 259-262.

Azaz, A. D. 2003. Isolation and identification of soil-borne fungi in fields irrigated by GAP in Harran Plain using two isolation methods. Turkish Journal of Botany, 27: 83-92.

Azeem, W., T. Mukhtar and T. Hamid. 2020. Evaluation of Trichoderma harzianum and Azadirachta indica in the Management of Meloidogyne incognita in Tomato. Pakistan Journal of Zoology, 53:1-7.

Berber, F., A. Ouazzani Touhami, A. Badoc and A. Douira. 2009. Antagonisme in vitro et in vivo de deux Trichoderma à l’égard de quatre espèces de Bipolaris pathogènes sur le sorgho. Bull. Soc. Pharm. Bordeaux, 148: 93-114.

Dennis, C. and J. Webster. 1971. Antagonistic properties of species-groups of Trichoderma. Transactions of the British Mycological Society, 57: 25-31.

Dubey, S. 2003. Integrated management of web blight of urd/mung bean by bio-seed treatment. Indian Phytopathology, 56: 34-38.

Elad, Y. 1980. Trichoderma harzianum: A Biocontrol Agent Effective Against Sclerotium rolfsii and Rhizoctonia solani. Phytopathology, 70: 119-125.

Elad, Y. 1983. Parasitism of Trichoderma spp. On Rhizoctonia solani and Sclerotium rolfsii-- Scanning Electron Microscopy and Fluorescence Microscopy. Phytopathology, 73: 85-91.

Elad, Y. 2000. Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Protection, 19: 709-714.

Ezziyyani, M., M. Requena, C. Egea Gilabert and M. Candela. 2007. Biological control of Phytophthora root rot of pepper using Trichoderma harzianum and Streptomyces rochei in combination. Journal of Phytopathology, 155: 342-349.

Harman, G. E., R. Petzoldt, A. Comis and J. Chen. 2004. Interactions between Trichoderma harzianum strain T22 and maize inbred line Mo17 and effects of these interactions on diseases caused by Pythium ultimum and Colletotrichum graminicola. Phytopathology, 94: 147-153.

Harrigan, W. F. 1998. Laboratory methods in food microbiology. Gulf professional publishing, Oxford, UK.

Hibar, K., M. Daami-Remadi, H. Khiareddine and M. El Mahjoub. 2005. Effet inhibiteur in vitro et in vivo du Trichoderma harzianum sur Fusarium oxysporum f. sp. radicis-lycopersici. Biotechnology, Agronomy, Society and Environment, 9, 163–171.

Howell, C. R. 2003. Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Plant Disease, 87: 4-10.

Hussain, A., A. Saboor, M. A. Khan, A. Q. Mohsin and F. Hassan. 2012. Technical efficiency of wheat production in rain-fed areas: a case study of Punjab, Pakistan. Pakistan Journal of Agricultural Sciences, 49: 411-417.

Ilyas, M. B., S. A. A. Bokhari and M. A. Khan. 1998. Fungi detected from wheat seeds exhibiting black points symptoms and their control by seed treatment. Pakistan Journal of Phytopathology, 10: 86-89.

Iqbal, U. and T. Mukhtar. 2020. Evaluation of biocontrol potential of seven indigenous Trichoderma species against charcoal rot causing fungus, Macrophomina phaseolina. Gesunde Pflanzen, 72: 195-202.

Jegathambigai, V., R. W. Wijeratnam and R. Wijesundera. 2009. Trichoderma as a seed treatment to control Helminthosporium leaf spot disease of Chrysalidocarpus lutescens. World Journal of Agricultural Sciences, 5: 720-728.

Khan, H. U., T. Mukhtar, R. Ahmad and N. Javed. 2004. Evaluation of synergistic effects of Paecilomyces lilacinus and Trichoderma harzianum against root-knot disease of tomato. Pakistan Journal of Phytopathology, 16: 13-16.

Küçük, Ç. and M. Kivanç. 2004. In vitro antifungal activity of strains of Trichoderma harzianum. Turkish Journal of Biology, 28: 111-115.

Lichtenzveig, J., J. Anderson, G. Thomas, R. Oliver and K. Singh. 2006. Inoculation and growth with soil borne pathogenic fungi. Medicago Truncatula, 4: 1-10.

Matti, D. and C. Sen. 1985. Integrated biocontrol of Sclerotium rolfsii with nitrogenous fertilizers and Trichoderma harzianum. Indian Journal of Agricultural Sciences, 55: 464-468.

Mukhtar, T. 2018. Management of root-knot nematode, Meloidogyne incognita, in tomato with two Trichoderma Species. Pakistan Journal of Zoology, 50: 1-6.

Mukhtar, T., M. Arshad Hussain and M. Zameer Kayani. 2013. Biocontrol potential of Pasteuria penetrans, Pochonia chlamydosporia, Paecilomyces lilacinus and Trichoderma harzianum against Meloidogyne incognita in okra. Phytopathologia Mediterranea, 20: 66-76.

Mukhtar, T., M. Tariq-Khan and M. N. Aslam. 2021. Bioefficacy of Trichoderma species against javanese root-knot nematode, Meloidogyne javanica, in Green Gram. Gesunde Pflanzen, 73: 265-272.

Muthomi, J. W., G. M. Riungu and J. M. Wagacha. 2007. Management of Fusarium head blight of wheat using antagonistic microorganisms. Department of Plant Science and Crop Protection, University of Nairobi, Kenya.

Patale, S. and D. Mukadam. 2011. Management of plant pathogenic fungi by using Trichoderma species. Bioscience Discovery Journal, 2: 36-37.

Perelló, A., C. Mónaco, M. R. Simón, M. Sisterna and G. D. Bello. 2003. Biocontrol efficacy of Trichoderma isolates for tan spot of wheat in Argentina. Crop Protection, 22: 1099-1106.

Perry, E. D., F. Ciliberto, D. A. Hennessy and G. Moschini. 2016. Genetically engineered crops and pesticide use in U.S. maize and soybeans. Science Advances, 2: e1600850-e1600850.

Rehman, A. 2011. Study of most prevalent wheat seed-borne mycoflora and its effect on seed nutritional value. African Journal of Microbiology Research, 5: 4328-4337.

Sabatini, M. A., P. Grazioso, C. Altomare and G. Innocenti. 2002. Interactions between Onychiurus armatus and Trichoderma harzianum in take-all disease suppression in a simple experimental system. European Journal of Soil Biology, 38: 71-74.

Sallam, N. M., K. Abo-Elyousr and M. Hassan. 2008. Evaluation of Trichoderma species as biocontrol agents for damping-off and wilt diseases of Phaseolus vulgaris L. and efficacy of suggested formula. Egypt Journal of Phytopatholog, 36: 81-93.

Sempere, F. and M. P. Santamarina. 2007. In vitro biocontrol analysis of Alternaria alternata (Fr.) Keissler under different environmental conditions. Mycopathologia, 163:183-190.

Sharma, K., A. K. Mishra and R. S. Misra. 2009. Morphological, biochemical and molecular characterization of Trichoderma harzianum isolates for their efficacy as biocontrol agents. Journal of Phytopathology, 157: 51-56.

Sivan, A. and I. Chet. 1989. Degradation of fungal cell walls by lytic enzymes of Trichoderma harzianum. Microbiology, 135: 675-682.

Tawfik, A. A. and A. D. Allam. 2004. Improving cumin production under soil infestation with Fusarium wilt pathogen: I-Screening of biocontrol agents. Assiut Univ. Bulliten of Environmental Research, 7: 35-44




DOI: https://doi.org/10.33866/phytopathol.035.01.0849

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Pakistan Journal of Phytopathology
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