MORPHOLOGICAL AND CULTURAL CHARACTERIZATION OF BOTRYTUS CINEREA CAUSING GRAY MOLD DISEASE OF LENTIL CROP FROM PAKISTAN

Nawab Zada, Gulshan Irshad, Farah Naz, Asim Gulzar, Muhammad Shahid, Muhammad F. Aslam, Salman Ghuffar

Abstract


The lentil (Lens culinaris) is an edible pulse crop and belongs to family Leguminosae. Fungal diseases of lentil are the most important biological constraint to productivity. Out of which Botrytis Gray mold causes significant losses. The present investigation is carried out to characterize Botrytis gray mold on lentil crop. During the survey 11 isolates of Botrytis Cinerea causing grey mold disease was collected different lentil growing areas of Pothwar regions. The isolates were identified on the basis of cultural and microscopic features. After 6 days during cultural studies maximum growth was observed on Czepk and Malt extract medium (85 mm) and minimum growth was on PDA (74.1 mm). The colony color was varies from whitish to black grayish with fluffy texture. Microscopically B. cinerea produces gray mycelium with branched conidiophores that that have rounded apical cells bearing cluster of colorless or gray one celled, ovoid conidia. The conidiophores and cluster of conidia resembles as a grape-like cluster. The pathogenecity test confirmed all the isolates of B. Cinerea were able to cause the gray mold disease. The overall objective of this study is to morphologically characterize the Botrytis cinera that provides a due step for management of Botrytis grey mould disease of lentil crops.


Keywords


Lentil; Botrytus cinerea; Isolation; Morphological and cultural characterization

Full Text:

PDF

References


Acero F.J.F. 2006. Application of proteomics to the characterization of mechanisms pathogenicity in Botrytis cinerea: Use and evaluation of new fungicides. Ph.D thesis, The University of Cadiz, Spain.

Ahmed A.U., S. Pande., A.K. Basandrai, G.K. Kishore and J.N. Rao. 2007. Variation in isolates of Botrytis cinerea causing Botrytis gray mold in chickpea. Bangladesh J. Agril. Res, 32(1):135-143.

Ahlawat, I.P.S. 2012. Agronomy Rabi crops, Lentil. Division of Agronomy Indian Agricultural Research Institute, New Delhi – 110 012 Agronomy.

Ali, J. A.F., M. A. Arain and N.A. Shaikh. 2010. Genetic manipulation of lentil through induced mutations. Pak. J. Bot. 42 (5): 3449-3455.

Brouwer, J. B., B. Sharma, B. A. Malik, and G. D. Hill. 2000. Region 6: Asia-Pacific: Meeting the challenge. In: Linking research and marketing opportunities for pulses in the 21st century. Proceedings of the third international food legumes research conference. Current Plant science and biotechnology in agriculture.(Ed R. Knight) Kluwer Academic Publishers. 34:115-129.

Cokkizgin, A. and M. J. Y. Shtaya. 2013.Lentil: Origin, Cultivation Techniques, Utilization and Advances in Transformation. Agri. SCI. 1 (1): 55-62.

Diolez, A., F. Marches, D. Fortini, and Y. Brygoo. 1995. Botrytis, a long-terminal-repeat retroelement in the phytopathogenic fungus Botrytis cinerea. Appl. Environ. Microbiol. 61: 103-108.

Ellis, M. B. 1971. Dematiacious hyphomycetes. CABI, Kew, England.608 pp.

Gossen, B.D., Lan. Z., Harrison. L.M., Holley. J and Smith. S.R. 1997. Survey of blossom blight of alfalfa on the Canadian Prairies. Can. Plant Dis. Surv.77: 88-89.

Haq, M. Z. U., S. Ahmad, M. A. Shad, S. Iqbal, M. Qayyum, A. Ahmad, D. L. Luthria and R. Amarowicz. 2011. Compositional studies of lentil (Lens culinaris Medik) cultivars commonly grown in Pakistan. Pak. J. Bot., 43 (3): 1563-1567.

Hawthorne, W., M. Materne, J. Davidson, K. Lindbeck, L. McMurray and J. Brand.2012. Lentil Disease Management Strategy. Southern pulse bulletin of Australia.50-55.

Hosen, M.I., A.U. Ahmed and M.R. Islam. 2010b. Physiological variability and in vitro antifungal activity against Botrytis cinerea causing botrytis gray mold of chickpea (Cicer arietinum L.).Spain J Agric Res., 8(3):750-756. http://dx.doi.org/10.5424/sjar/2010083-1274.

Ivanova, H., S. Bernadovicova. 2010. Species diversity of microscopic fungi on Austrian pines growing in urban greenery of Nitra town. Folia Oecologica, 37(2):168.

Morsy, K. M. M. 2005.Induced Resistance against Damping-off, Root Rot and Wilt Diseases of Lentil. PlantPathol. 33: 53-63

Muehlbauer, F.J. 1991. Use of introduced germplasm in cool season food legume cultivar development.

Pande, S., M. Sharma, G. Kishore, L.S. Krishna and U.M. Naga . 2010. Characterization of Botrytis cinerea isolates from chickpea: DNA polymorphisms, cultural, morphological and virulence characteristics. African Journal of Biotechnology, 9(46):7961-7967.

Pande, S., G. Galloway, P. M. Gaur, K. H. M. Siddique, H. S. Tripathi, P. Taylor, M. W. J. MacLeod, A. K. Basandrai, A. Bakr, S. Joshi, G. K. Kishore, D. A. Isenegger, R. J. Narayana and Smith, D., A.H.S. Onions. 1983. A comparision of some preservation techniques for fungi. Trans. Br. Mycol. Soc. 81 (3): 535-540.

Stoilova., T. Petrova, N. Penov And A. Krastanov. 2013. Antioxidant activity of lentil semolina extrudates using cathode voltammetry. BJAS. 19 (1): 15-21.

Williamson, B., Tudzynsk, B., Tudzynski, P., and van Kan, J. A. L. 2007. Botrytis cinerea: the cause of grey mould disease. Molecular Plant Pathology.8: 561 -580.

www.planthealthaustralia.com.au/biosecurity/grains


Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Nawab Zada, Gulshan Irshad, Farah Naz, Asim Gulzar, Muhammad Shahid, Muhammad F. Aslam, Salman Ghuffar

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

      
   
Pakistan Journal of Phytopathological
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.