Bilal Ahmed, Babar Khan, Muhammad U. Ghazanfar, Nasir A. Rajput, Abdul Jabbar, Waqar Ahmad, Madassar Walait


Vegetables seeds are carrier of mycoflora inciting bulk of diseases, responsible for quality and yield losses. Majority of microbes are saprophyte and few are potential pathogen for the crop. In this research seed mycoflora were isolated by using standard blotter paper and agar plate technique. Major vegetables of summer and winter seeds were collected from regional local markets of Punjab province. Among these 15 fungal genera and 18 different species were identified. According to results highest incidence of fungal pathogens were Aspergillus niger, A. flavus, Penicillium camemberti and Bipolaris spp. and low incidence of Stemphylium spp., Cladosporim spp., F. semitectum, Curvularia lunata, Rhizopus nigricans, Paecilomyces lilacinus, Fusarium oxysporum, Drechslera australiensis, Ascochyta spp., A. fumigatus, Rhizoctonia spp., Alternaria alternata and Chaetomium globosum. The agar plate method was found to be the most suitable technique for detection seed-borne fungi in vegetable seed. The results of this study could be helpful for management of seed borne fungal pathogens of different vegetables. Our findings may also helpful for seed treatment before sowing with appropriate fungicides to overcome the losses caused by seed borne fungi.


Saprophytes, Pathogenic, Detection, Isolation, Identification, Purification

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Abawi, G. S. and T. L. Widmer. 2000. Impact of soil health management practices on soilborne pathogens, nematodes and root diseases of vegetable crops. Applied Soil Ecology, 15: 37-47.

Agarwal, V. K. and J. B. Sinclair. 1996. Principles of seed pathology. CRC Press.

Ali, M. and S. Kumar. 2000. Problems and prospects of pulses research in India. Indian Farm, 50: 4-13.

Anjorin, S. and M. Mohammed. 2009. Effects of seed-borne fungi on germination and seedling growth of watermelon (Citrullus lanatus). Journal of Agriculture and Social Sciences, 5: 77-80.

Association, I. S. T. 1993. Intermediate rules for seed testing. Seed Science and Technology, 21: 37-41.

Avinash, T. and V. RavishankarRai. 2013. Identification of diverse fungi related with selected cucurbitaceae vegetables. Journal of Agricultural Technology, 9: 1837-1848.

Bakhsh, K. 2007. An analysis of technical efficiency and profitability of growing potato, carrot, radish and bitter gourd: A case study of Pakistani Punjab. Unpublished Ph. D. Dissertation, Department of Farm Management, University of Agriculture, Faisalabad, Pakistan.

Begum, H. and A. Momin. 2000. Comparison between two detection techniques of seed-borne pathogens in cucurbits in Bangladesh. Pakistan Journal of Scientific and Industrial Research, 43: 244-248.

Booth, C. 1971. Chapter II Fungal Culture Media. Methods in Microbiology. Elsevier, pp. 49-94.

Broders, K. D., P. E. Lipps, P. A. Paul and A. E. Dorrance. 2007. Evaluation of Fusarium graminearum Associated with Corn and Soybean Seed and Seedling Disease in Ohio. Plant Disease, 91: 1155-1160.

Chavan, A. M. 2011. Nutritional changes in oilseeds due to Aspergillus spp. Journal of Experimental Sciences, 2.

Dawson, W. A. J. M. and G. L. Bateman. 2001. Fungal communities on roots of wheat and barley and effects of seed treatments containing fluquinconazole applied to control take-all. Plant Pathology, 50: 75-82.

Elwakil, M. A. and M. A. El-Metwally. 2001. Seed-Borne Fungi of Peanut in Egypt: Pathogenicity and Transmission. Pakistan Journal of Biological Sciences, 4: 63-68.

Fakhrunnisa, M. H. and A. Ghaffar. 2006. In vitro interaction of Fusarium spp., with other fungi. Pakistan Journal of Botany, 38: 1317-1322.

Fakir, G. 1983. Teaching, training and research activities of seed pathology in Bangladesh. Seed Science and Technology.

Frisvad, J. C. and R. A. Samson. 2004. Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Studies in mycology, 49: C174.

Ijaz, A., S. Anwar, A. Riaz and M. Khan. 2001. Seed borne pathogens associated with wheat seed and their role in poor germination. Pakistan Journal of Phytopathology, 13: 102-106.

Khan, S., A. Khanzada, S. Nasreen and M. Aslam. 1988. Evaluation of seed health testing techniques for the assessment of seed-borne mycoflora of rice. Pakistan Journal of Agricultural Research, 9: 502-505.

Koike, S. T., P. Gladders and A. Paulus. 2006. Vegetable diseases: A colour handbook. CRC Press.

Kubiak, K. and M. Korbas. 1999. Occurrence of fungal diseases on selected winter wheat cultivars. Postepy Ochronie Roslin, 39: 801-804.

Leslie, J. F., K. A. Zeller, S. C. Lamprecht, J. P. Rheeder and W. F. O. Marasas. 2005. Toxicity, Pathogenicity, and Genetic Differentiation of Five Species of Fusarium from Sorghum and Millet. Phytopathology, 95: 275-283.

Liu, B., L. Giesler, T. Jackson-Ziems, S. Wegulo, R. Harveson, K. Korus and R. Klein. 2013. Major Fusarium diseases on corn, wheat and soybeans in Nebraska.

Logrieco, A., A. Bottalico, G. Mulé, A. Moretti and G. Perrone. 2003. Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops. Epidemiology of Mycotoxin Producing Fungi. Springer Netherlands, pp. 645-667.

Mathur, S. 1990. Summaries of research project 1967-1988. Danish Govt. Inst. of Seed Path. For Dev. Countries, Denmark, 111.

Mathur, S. 2003. Common Laboratory Seed Health Testing Methods for Detecting Fungi. International Seed Testing Association.

Mittal, R. K. 1983. Studies on the mycoflora and its control on the seeds of some forest trees. I. Cedrus deodara. Canadian Journal of Botany, 61: 197-201.

Naeem, K., S. Anwar, M. Haque, A. Riaz and M. Khan. 2001. Seed-borne fungi and bacteria of rice and their impact on seed germination. Pakistan Journal of Phytopathology, 13: 75-81.

NELSON, P. M. and T. WFO TOUSSOUN. 1983. Fusarium Species: an Illustred Manual for Identification. The Pennsylvania State University.

Niaz, I. and S. Dawar. 2009. Detection of seed borne mycoflora in maize (Zea mays L.). Pakistan Journal of Botany, 41: 443-451.

Ozcelik, S., N. Ozcelik and L. R. Beuchat. 1990. Toxin production by Alternaria alternata in tomatoes and apples stored under various conditions and quantitation of the toxins by high-performance liquid chromatography. International Journal of Food Microbiology, 11: 187-194.

Rahim, S. M. and S. S. Hussain. 2013. Studies on stored grain fungi Part I. Fungi on wheat and rice from Karachi. Pakistan Journal of Scientific and Industrial Research, 11: 375-387.

Raper, K. and D. Fennel. 1965. Aspergillus terreus group. The genus Aspergillus. The Williams & Wilkins Co., Baltimore, Md.

Sonni, A. 2002. Importance of minerals and trace minerals in human nutrition. Website: www. mgwater. com/imp or. Shtml (15 April, 2004).

Sultana, N. and A. Ghaffar. 2007. Seed-borne fungi associated with bitter-gourd (Momordica charantia Linn.). Pakistan Journal of Botany, 39: 2121-2125.

Yuan, Z. Q., K. M. Old, S. J. Midgley and D. Solomon. 1997. Mycoflora and pathogenicity of fungi present on stored seeds from provenances of Eucalyptus pellita. Australasian Plant Pathology, 26: 195.

Zitter, T. A. and C. DL Thomas. 1996. Compendium of cucurbit diseases. American Phytopathological Society, St. Paul, Minn.(EUA).

DOI: https://doi.org/10.33866/phytopathol.029.02.0405


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Pakistan Journal of Phytopathology
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