Mesele Admassie, Tesefaye Alemu, Aferasa Mulatu


Biological control is the suppression of damaging activities of one organism by one or more other organisms. They are many environmental benefits of biological control including safety for humans and other non-target organisms, reduction of pesticide residues in food, increased activity of most other natural enemies, and increased biodiversity in managed ecosystems, their advantages are numerous. microorganism–microorganism or microorganism–host interactions involve all ecological aspects, including physiochemical changes, metabolite exchange, metabolite conversion, signaling, chemotaxis and genetic exchange resulting in genotype selection. Microbial interactions occur by the transference of molecular and genetic information, and many mechanisms can be involved in this exchange, such as secondary metabolites, siderophores, quorum sensing system, bio-film formation, and cellular transduction signaling, among others. The agricultural importance of the biocontrol is that possess good antagonistic abilities against plant pathogenic microbes. Antagonism is based on different mechanisms, like the production of antifungal metabolites, competition for space and nutrients and myco-parasitism. Some of microbes like Trichoderma strains with effective antagonistic abilities are potential candidates for the biological control of plant diseases. Biotic and biotic environmental parameters may have negative influence on the bio-control efficacy of biocontrol strains.


Biological control; physiochemical; Microbial interactions; quorum sensing; biofilm formation; antagonistic

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Ahmad, J. S. 1987. Rhizosphere Competence of Trichoderma harzianum. Phytopathology, 77: 182.

Ahn, I.-P., K. Park and C.-H. Kim. 2002. Rhizobacteria-induced resistance perturbs viral disease progress and triggers defense-related gene expression. Molecules and cells, 13: 302-308.

Al-Rawahi, A. K. and J. G. Hancock. 1998. Parasitism and Biological Control of Verticillium dahliae by Pythium oligandrum. Plant Disease, 82: 1100-1106.

Apel, K. and H. Hirt. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55: 373-399.

Azevedo, J. L., W. L. Araújo and P. T. Lacava. 2016. The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants. Genetics and Molecular Biology, 39: 476-491.

Benhamou, N., S. Gagné, D. Le Quéré and L. Dehbi. 2000. Bacterial-mediated induced resistance in cucumber: beneficial effect of the endophytic bacterium Serratia plymuthicaon the protection against Infection by Pythium ultimum. Phytopathology, 90: 45-56.

Bhat, K. A. 2017. A New Agar Plate Assisted Slide Culture Technique to Study Mycoparasitism of Trichoderma sp. on Rhizoctonia solani and Fusarium oxysporium. International Journal of Current Microbiology and Applied Sciences, 6: 3176-3180.

Braga, R. M., M. N. Dourado and W. L. Araújo. 2016. Microbial interactions: ecology in a molecular perspective. Brazilian Journal of Microbiology, 47: 86-98.

Cardon, Z. G. and D. J. Gage. 2006. Resource Exchange in the Rhizosphere: Molecular Tools and the Microbial Perspective. Annual Review of Ecology, Evolution, and Systematics, 37: 459-488.

Cartwright, D. K. and H. W. Spurr. 1998. Biological control of Phytophthora parasitica var. Nicotianae on tobacco seedlings with non-pathogenic binucleate Rhizoctonia fungi. Soil Biology and Biochemistry, 30: 1879-1884.

Chen, B. and D. L. Nuss. 1999. Infectious cDNA clone of hypovirus CHV1-Euro7: a comparative virology approach to investigate virus-mediated hypovirulence of the chestnut blight fungus Cryphonectria parasitica. Journal of Virology, 73: 985-992.

Chen, C., R. R. Bélanger, N. Benhamou and T. C. Paulitz. 1998. Induced systemic resistance (ISR) by Pseudomonas spp. impairs pre-and post-infection development of Pythium aphanidermatum on cucumber roots. European Journal of Plant Pathology, 104: 877-886.

Chin-A-Woeng, T. F. C., G. V. Bloemberg, A. J. van der Bij, K. M. G. M. van der Drift, J. Schripsema, B. Kroon, R. J. Scheffer, C. Keel, P. A. H. M. Bakker, H.-V. Tichy, F. J. de Bruijn, J. E. Thomas-Oates and B. J. J. Lugtenberg. 1998. Biocontrol by phenazine-1-carboxamide-Producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f.sp. radicis-lycopersici. Molecular Plant-Microbe Interactions, 11: 1069-1077.

Constantinescu, F., O.-A. Sicuia, C. Fătu, M. M. Dinu, A.-M. Andrei and C. Mincea. 2014. In vitro compatibility between chemical and biological products used for seed treatment. Scientific Papers Series A Agronomy, 57: 146-151.

Da Luz, W., G. Bergstrom and C. Stockwell. 1998. Seed-applied bioprotectants for control of seedborne Pyrenophora tritici-repentis and agronomic enhancement of wheat. Canadian journal of plant pathology= Revue Canadienne de phytopathologie.

Davanlou, Madsen, Madsen and Hockenhull. 1999. Parasitism of macroconidia, chlamydospores and hyphae of Fusarium culmorum by mycoparasitic Pythium species. Plant Pathology, 48: 352-359.

De Boer, M., I. van der Sluis, L. C. van Loon and P. A. Bakker. 1999. Combining fluorescent Pseudomonas spp. strains to enhance suppression of fusarium wilt of radish. European Journal of Plant Pathology, 105: 201-210.

De, C., L. Garcia, Pascual and Melgarejo. 1999. Effects of timing and method of application of Penicillium oxalicum on efficacy and duration of control of Fusarium wilt of tomato. Plant Pathology, 48: 260-266.

Delgado-Jarana, J., J. A. Pintor-Toro and T. Benítez. 2000. Overproduction of β-1, 6-glucanase in Trichoderma harzianum is controlled by extracellular acidic proteases and pH. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1481: 289-296.

Duczek, L. J. 1997. Biological control of common root rot in barley by Idriella bolleyi. Canadian Journal of Plant Pathology, 19: 402-405.

Duijff, B. J., D. Pouhair, C. Olivain, C. Alabouvette and P. Lemanceau. 1998. European Journal of Plant Pathology, 104: 903-910.

Ebrahim, S., K. Usha and B. Singh. 2011. Pathogenesis related (PR) proteins in plant defense mechanism. Sci Against Microb Pathog, 2: 1043-1054.

Engelkes, C. A., R. L. Nuclo and D. R. Fravel. 1997. Effect of Carbon, Nitrogen, and C:N Ratio on Growth, Sporulation, and Biocontrol Efficacy of Talaromyces flavus. Phytopathology, 87: 500-505.

Fisher, J. B., S. Sweeney, E. R. Brzostek, T. P. Evans, D. J. Johnson, J. A. Myers, N. A. Bourg, A. T. Wolf, R. W. Howe and R. P. Phillips. 2016. Tree-mycorrhizal associations detected remotely from canopy spectral properties. Global Change Biology, 22: 2596-2607.

Frank, B. (1885). Über die auf Wurzelsymbiose beruhende Ernährung gewisser Bäume durch unterirdische Pilze. Berichte der Deutschen Botanischen Gesellschaft, 3: 128-145.

Frey-Klett, P., P. Burlinson, A. Deveau, M. Barret, M. Tarkka and A. Sarniguet. 2011. Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists. Microbiology and Molecular Biology Reviews, 75: 583-609.

Fuchs, J. G., Y. Moënne-Loccoz and G. Défago. 1999. Ability of non-pathogenic Fusarium oxysporum Fo47 to Protect Tomato against Fusarium Wilt. Biological Control, 14: 105-110.

Giesler, L. J. and G. Y. Yuen. 1998. Evaluation of Stenotrophomonas maltophilia strain C3 for biocontrol of brown patch disease. Crop Protection, 17: 509-513.

Haldar, S. and S. Sengupta. 2015. Plant-microbe Cross-talk in the Rhizosphere: Insight and Biotechnological Potential. The Open Microbiology Journal, 9: 1-7.

Harris, A. R. and S. Nelson. 1999. Progress towards integrated control of damping-off disease. Microbiological Research, 154: 123-130.

Hebbar, K., M. Martel and T. Heulin. 1998. Suppression of pre-and postemergence damping-off in corn by Burkholderia cepacia. European journal of plant pathology, 104: 29-36.

Hervás, A., B. Landa, L. E. Datnoff and R. M. Jiménez-Dı́az. 1998. Effects of commercial and indigenous microorganisms on fusarium wilt development in chickpea1. Biological Control, 13: 166-176.

Hillman, B. I. and N. Suzuki. 2004. Viruses of the Chestnut Blight Fungus, Cryphonectria parasitica. Advances in Virus Research. Elsevier, pp. 423-472.

Holmberg, A.-I. J. 2011. Tracking the fate of biocontrol microorganisms in the environment using intrinsic SCAR markers.

Holmes, K. A., S. D. Nayagam and G. D. Craig. 1998. Factors affecting the control of Pythium ultimum damping‐off of sugar beet by Pythium oligandrum. Plant Pathology, 47: 516-522.

Howell, C. R. 1999. Selective isolation from soil and separation In Vitro of P and Q strains of Trichoderma virens with differential media. Mycologia, 91: 930.

Huang, B. and J.Y. Liu. 2006. Cloning and functional analysis of the novel gene GhDBP3 encoding a DRE-binding transcription factor from Gossypium hirsutum. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 1759: 263-269.

Jackson, A., J. Whipps and J. Lynch. 1991. Effects of temperature, pH and water potential on growth of four fungi with disease biocontrol potential. World Journal of Microbiology and Biotechnology, 7: 494-501.

Johnsson, L., M. Hökeberg and B. Gerhardson. 1998. Performance of the Pseudomonas chlororaphis biocontrol agent MA 342 against cereal seed-borne diseases in field experiments. European Journal of Plant Pathology, 104: 701-711.

Khan, N. I., A. B. Filonow and L. L. Singleton. 1997. Augmentation of soil with sporangia of Actinoplanes spp. for Biological Control of Pythium Damping-off. Biocontrol Science and Technology, 7: 11-22.

Khmel, I. A., T. A. Sorokina, N. B. Lemanova, V. A. Lipasova, O. Z. Metlitski, T. V. Burdeinaya and L. S. Chernin. 1998. Biological control of crown gall in grapevine and raspberry by two Pseudomonas spp. with a wide spectrum of antagonistic activity. Biocontrol Science and Technology, 8: 45-57.

Knoester, M., C. M. J. Pieterse, J. F. Bol and L. C. Van Loon. 1999. Systemic Resistance in Arabidopsis Induced by Rhizobacteria Requires Ethylene-Dependent Signaling at the Site of Application. Molecular Plant-Microbe Interactions, 12: 720-727.

Koch, E. 1999. Evaluation of commercial products for microbial control of soil-borne plant diseases. Crop Protection, 18: 119-125.

Kredics, L., Z. Antal, L. Manczinger, A. Szekeres, F. Kevei and E. Nagy. 2003. Influence of environmental parameters on Trichoderma strains with biocontrol potential. Food Technology and Biotechnology, 41: 37-42.

Kubicek, C., R. Mach, C. Peterbauer and M. Lorito. 2001. Trichoderma: from genes to biocontrol. Journal of Plant Pathology: 11-23.

Kwak, K. J., J. Y. Kim, Y. O. Kim and H. Kang. 2007. Characterization of transgenic arabidopsis plants overexpressing high mobility group B proteins under high salinity, drought or cold stress. Plant and Cell Physiology, 48: 221-231.

Larkin, R. P. and D. R. Fravel. 1998. Efficacy of various fungal and bacterial biocontrol organisms for control of Fusarium wilt of Tomato. Plant Disease, 82: 1022-1028.

Léon-Kloosterziel, K. M., B. W. M. Verhagen, J. J. B. Keurentjes, J. A. Van Pelt, M. Rep, L. C. Van Loon and C. M. J. Pieterse. 2005. Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle. Plant Molecular Biology, 57: 731-748.

Lewis, J. A. and R. P. Larkin. 1998. Formulation of the biocontrol fungus Cladorrhinum foecundissimum to Reduce Damping-Off Diseases Caused by Rhizoctonia solani and Pythium ultimum1. Biological Control, 12: 182-190.

Lima, A. L. 2002. Molecular and biochemical characterization of Trichoderma isolated from Brazilian cerrado soil. Ph.D Thesis. Universidad de Brasília, Brasilia, Brazil.

Lo, C. T., E. B. Nelson, C. K. Hayes and G. E. Harman. 1998. Ecological studies of transformed Trichoderma harzianum strain 1295–22 in the rhizosphere and on the phylloplane of creeping bentgrass. Phytopathology, 88: 129-136.

MacDonald, W. L. 1991. Biological Control of Chestnut Blight: Use and limitations of transmissible hypovirulence. Plant Disease, 75: 653.

Mathre, D. E., R. J. Cook and N. W. Callan. 1999. From Discovery to Use:Traversing the World of Commercializing Biocontrol Agents for Plant Disease Control. Plant Disease, 83: 972-983.

Menendez, A. B. and A. Godeas. 1998. Biological control of Sclerotinia sclerotiorum attacking soybean plants. Degradation of the cell walls of this pathogen by Trichoderma harzianum (BAFC 742). Mycopathologia, 142: 153-160.

Milgroom, M. G. and P. Cortesi. 2004. Biological control of chestnut blight with hypervirulence: A Critical Analysis. Annual Review of Phytopathology, 42: 311-338.

Muthulakshmi, M., B. Anita and S. Subramanian. 2017. Studies on compatibility of egg parasitic fungi with other biocontrol agents and carbofuran under in vitro.

Narisawa, Ohki and Hashiba. 2000. Suppression of clubroot and Verticillium yellows in Chinese cabbage in the field by the root endophytic fungus, Heteroconium chaetospira. Plant Pathology, 49: 141-146.

Nautiyal, C. S. 2006. Biological control of plant diseases by natural and genetically engineered fluorescent Pseudomonas spp, Microbial Biotechnology in Horticulture, Vol. 1. CRC Press, pp. 139-155.

Neeno-Eckwall, E. C. and J. L. Schottel. 1999. Occurrence of antibiotic resistance in the biological control of potato scab disease. Biological Control, 16: 199-208.

Nuss, D. L. 1996. Using hypoviruses to probe and perturb signal transduction processes underlying fungal pathogenesis. The plant cell online, 8: 1845-1853.

Oliveira, M. D. M., C. M. R. Varanda and M. R. F. Félix. 2016. Induced resistance during the interaction pathogen x plant and the use of resistance inducers. Phytochemistry Letters, 15: 152-158.

Oliveira, M., C. Varanda and M. Félix. 2016. Induced resistance during the interaction pathogen x plant and the use of resistance inducers. Phytochemistry letters, 15: 152-158.

Ouda, M. 2014. Biological control by microorganisms and ionizing radiation. International Journal of Advanced Research, 2: 314-356.

Pal, K. K. and B. McSpadden Gardener. 2006. Biological Control of Plant Pathogens. The Plant Health Instructor.

Pankhurst, C. E. and J. M. Lynch. 2005. Biocontrol of soil-borne plant diseases. Encyclopedia of soils in the environment. Elsevier, pp. 129-136.

Peever, T. L., Y.-C. Liu, K. Wang, B. I. Hillman, R. Foglia and M. G. Milgroom. 1998. Incidence and diversity of double-stranded RNAs occurring in the chestnut blight fungus,Cryphonectria parasitica, in China and Japan. Phytopathology, 88: 811-817.

Rajapaksha, R. M. C. P., M. A. Tobor-Kaplon and E. Baath. 2004. Metal toxicity affects fungal and bacterial activities in soil differently. Applied and Environmental Microbiology, 70: 2966-2973.

Ross, R. E., A. P. Keinath and M. A. Cubeta. 1998. Biological control of wire stem on cabbage using binucleate Rhizoctonia spp. Crop Protection, 17: 99-104.

Ryder, M. H., Z. Yan, T. E. Terrace, A. D. Rovira, W. Tang and R. L. Correll. 1998. Use of strains of Bacillus isolated in China to suppress take-all and rhizoctonia root rot, and promote seedling growth of glasshouse-grown wheat in Australian soils. Soil Biology and Biochemistry, 31: 19-29.

Sakuma, Y., K. Maruyama, F. Qin, Y. Osakabe, K. Shinozaki and K. Yamaguchi-Shinozaki. 2006. Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proceedings of the National Academy of Sciences, 103: 18822-18827.

Sharifi-Tehrani, A., M. Zala, A. Natsch, Y. Moënne-Loccoz and G. Défago. 1998. Biocontrol of soil-borne fungal plant diseases by 2, 4-diacetylphloroglucinol-producing fluorescent pseudomonas with different restriction profiles of amplified 16S rDNA. European Journal of Plant Pathology, 104: 631-643.

Sharma, A., V. Diwevidi, S. Singh, K. K. Pawar, M. Jerman, L. Singh, S. Singh and D. Srivastawav. 2013. Biological control and its important in agriculture. International Journal of Biotechnology and Bioengineering Research, 4: 175-180.

Sharma, D. and Y. Singh. 2018. Compatibility of Bio-control agents with bactericides for controlling bacterial wilt of tomato. IJCS, 6: 1173-1178.

Singh, P. P., Y. C. Shin, C. S. Park and Y. R. Chung. 1999. Biological control of Fusarium wilt of cucumber by Chitinolytic bacteria. Phytopathology, 89: 92-99.

Sreenivasulu, N., S. K. Sopory and P. B. Kavi Kishor. 2007. Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches. Gene, 388: 1-13.

Srinivasulu, M. and D. R. Ortiz. 2017. Effect of pesticides on bacterial and fungal populations in ecuadorian tomato cultivated soils. Environmental Processes, 4: 93-105.

Suryanarayanan, T. and R. U. Shaanker. 2015. Fungal endophytes-biology and bioprospecting preface. Indian Acad Sciences Cv Raman Avenue, Sadashvangar, PB# 8005, Bangalore.

Tapwal, A., R. Kumar, N. Gautam and S. Pandey. 2012. Compatibility of Trichoderma viride for selected fungicides and botanicals. International Journal of Plant Pathology, 3: 89-94.

Teetes, G. 1996. Plant resistance to insects: a fundamental component of IPM. Radcliffe’s IPM world textbook’.(Eds EB Radcliffe, WD Hutchison, RE Cancelado)(University of Minnesota: St Paul) Available at http://ipmworld. umn. edu/chapters/teetes. htm [Verified 7 October 2008].

Thompson, D. C., D. Y. Kobayashi and B. B. Clarke. 1998. Suppression of summer patch by rhizosphere competent bacteria and their establishment on Kentucky bluegrass. Soil Biology and Biochemistry, 30: 257-263.

Thompson, D. C., D. Y. Kobayashi and B. B. Clarke. 1998. Suppression of summer patch by rhizosphere competent bacteria and their establishment on Kentucky bluegrass. Soil Biology and Biochemistry, 30: 257-263.

Timmusk, S., P. van West, N. A. Gow and G. E. Wagner. 2003. Antagonistic effects of Paenibacillus polymyxa towards the oomycete plant pathogens Phytophthora palmivora and Pythium aphanidermatum. In: Mechanism of action of the plant growth promoting bacterium Paenibacillus polymyxa, pp. 1-28. Department of Cellular and Molecular Biology. University of Uppsala, Sweden, ISBN 91 554 5802 5.

Utkhede, R. S. and C. A. Koch. 1999. Rhizobacterial growth and yield promotion of cucumber plants inoculated with Pythium aphanidermatum. Canadian Journal of Plant Pathology, 21: 265-271.

Vashisht, A. A. and N. Tuteja. 2006. Stress responsive DEAD-box helicases: A new pathway to engineer plant stress tolerance. Journal of Photochemistry and Photobiology B: Biology, 84: 150-160.

Vidhyasekaran, P. and M. Muthamilan. 1999. Evaluation of a powder formulation of Pseudomonas fluorescens Pf1 for control of rice sheath blight. Biocontrol Science and Technology, 9: 67-74.

Vinocur, B. and A. Altman. 2005. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Current Opinion in Biotechnology, 16: 123-132.

Warren, J. E. and M. A. Bennett. 1999. Bio-osmopriming tomato (Lycopersicon esculentum Mill.) seeds for improved stand establishment. Seed science and technology, 27: 489-499.

Weindling, R. 1932. Trichoderma lignorum as a parasite of other soil fungi. Phytopathology, 22: 837-845.

Whipps, J. M. 2001. Microbial interactions and biocontrol in the rhizosphere. Journal of Experimental Botany, 52: 487-511.

Whipps, J. M. 2001. Microbial interactions and biocontrol in the rhizosphere. Journal of Experimental Botany, 52: 487-511.

Whipps, J. M. and R. D. Lumsden. 2001. Commercial use of fungi as plant disease biological control agents: status and prospects. Fungal biocontrol agents: progress, problems and potential: 9-22.

Woo, S. L., F. Scala, M. Ruocco and M. Lorito. 2006. The Molecular Biology of the Interactions between Trichoderma spp., Phytopathogenic Fungi, and Plants. Phytopathology, 96: 181-185.

Yu, X., B. Li, Y. Fu, D. Jiang, S. A. Ghabrial, G. Li, Y. Peng, J. Xie, J. Cheng and J. Huang. 2010. A Gemini virus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proceedings of the National Academy of Sciences, 107: 8387-8392.

DOI: https://doi.org/10.33866/phytopathol.031.01.0495


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