Senthilkumar Palanisamy, Suganthi Muthusamy, A.K.A Mandal


Tea is one among the non-alcoholic beverages consumed by people across the continents. As tea is a monocrop, it serves a haven for numerous pests and pathogens. Being a timeless plant, the agronomical practices and stable microclimate favours the disease development. Among the diseases affecting the leaves, grey blight – courtesy of Pestalotiopsis theae- is one of the most vital and detrimental diseases in tea. Plant modifies its arsenal system against the pathogens to produce higher levels of antimicrobial products which includes pathogenesis proteins. Understanding the expression pattern of these proteins will help in developing disease tolerance cultivars. All the three pathogenesis-related proteins exhibited higher expression in the impaired cultivars, while the sensitive cultivars showed lesser degree of enzyme concentration. Expression pattern of pathogenesis-related proteins like Phenylalanine ammonia-lyase, β-1,3 glucanases and chitinase were reported and discussed greatly.Activity of all three enzymes were found to be increased in resistant cultivars than the sensitive cultivars. The enzyme activity was slightly higher in the infected plants when compared with the uninfected plants.


Pestalotiopsis theae; Camellia sinensis; Pathogenesis related proteins; grey blight

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Anguelova, V.S., A.J. Westhurzen and Z.A.D. Pretorius. 1999. Intercellular proteins and β-1, 3-glucanase activity associated with leaf rust resistance in wheat. Physiologia Plantarum, 106: 393-401.

Ano, A., Takayanagi, T., Uchibori, T., T. Okuda and K. Yokotsuka. 2000. Characterization of a classIII chitinase from Vitis vinifera cv. Koshu. Journal of Bioscience and Bioengineering 95: 645–647.

Bartnicki-Garcia, S. 1968. Cell Wall Chemistry, Morphogenesis, and Taxonomy of Fungi. Annual Review of Microbiology, 22: 87-108.

Bolar, J.P., Norelli, J.L., Harman, G.E., S.K. Brown and H.S. Aldwinckle. 2001. Synergistic activity of endochitinase and exochitinase form Trichoderma atroviride (T. harzianum) against the pathogenic fungus (Venturia ineaualis) in transgenic ale plants. Trans Research, 10: 533-543.

Boller, T. 1987. Hydrolytic enzymes in plant disease. In Plant-Microbe Interactions, Molecular and Genetic Perspectives. Eds. Kosuge, T. and E.W. Nester. Macmillan Publishing Company, New York, USA. 385-413.

Boller, T. 1988. Extracellular localization of chitinase in cucumber. Physiology and Molecular Plant Pathology, 33, 11-16.

Boller, T., Gehri, A., F. Mauch and U. Vogeli. 1983. Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function. Planta, 157(1): 22-31.

Bowles, D.J. 1990. Defense-related proteins in higher plants. Annual Review of Biochemistry, 59: 873-907.

Bradford, M. 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Annals of Biochemistry, 72: 248-254.

Broekaert, W.F., Parijs, J.V., K.A. Allen and W.J. Peumans. 1988. Comparison of some molecular, enzymatic and antifungal properties of chitinase from thorn-ale, tobacco and wheat. Physiology and Molecular Plant Pathology, 33: 319-331.

Chen, C., Belanger, R.R., N. Benhamou and T.C. Paulitz. 2000. Defense enzymes induced in cucumber roots by treatment with plant growth promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiological and Molecular Plant Pathology, 56: 13–23.

Chen, J.S. and X.F. Chen. 1990. The Diagnosis of Tea Diseases and their control (in Chinese) Shanghai. Shanghai scientific and technical publishers, Shanghai, China. 32-41

Daayf, F., R. Bel-Rhlid and R.R. Belanger. 1997. Methyl ester of p-coumaric acid: A phytoalexin-like compound from long English cucumber leaves. Journal of Chemical Ecology, 23: 1517–1526.

Ferraris, L., G.I. Abbattista and A. Matt. 1987. Activation of Glycosidases as a Consequence of Infection Stress in Fusarium Wilt of Tomato. Journal of Phytopathology, 118(4): 317-325.

Fink, W., M. Liefland and K. Mendgen. 1988. β-1, 3-glucanase in the apoplastic compartment of oat leaves (Avena sativa). Plant Physiology, 88: 270-275.

Garcia-Garcia, F., E. Schmeizer and K. Hahlbrock. 1994. Differential expression of chitinases and β -1,3 glucanase genes in various tissues of potato plants. Zeitschrift für Naturforschung 49: 195-203.

Huynh, Q.K., Hironaka, C.M., Levine, E.B., Smith, C.E., J.R. Borgmeyer and D.M. Shah. 1992. Antifungal proteins from plants-purification, molecular-cloning, and antifungal properties of chitinases from maize seed. Journal of Biological Chemistry, 267(10): 6635-6640.

Jebakumar, R.S., M. Anandaraj and Y.R. Sarma. 2001. Induction of PR proteins and defense-related enzymes in black pepper due to inoculation with Phytophthora capsici. Indian Phytopathology, 54 (1): 23-28.

Kanrara, S., Venkateswara, J.C., P.B. Kirtib and V.L. Chopra. 2002. Transgenic expression of hevein, the rubber tree lectin, in Indian mustard confers protection against Alternaria brassicae. Plant Science, 162: 441-448.

Kervinen, T., Peltonen, S., T.H. Teeri and R. Karjalainen. 1998. Differential phenylalanine ammonia lyase genes in barley induced by fungal infection or elicitors. New Phytologist, 139(2): 293-300.

Kim, Y.J. and B.K. Hwang. 1994. Differential accumulation of β -1,3 glucanases and chitinase isoforms in peer stems infected by compatible and incompatible isolates of Phytophthora capsici. Physiological and Molecular Plant Pathology, 45: 195-209.

Koh, Y.J., G.H. Shin and J.S. Hur. 2001. Seasonal Occurrence and Development of Gray Blight of Tea Plants in Korea. Plant Pathology Journal. 17(1): 40-44.

Lee, H. I. and N.V. Raikhel. 1995. Prohevein is poorly processed but shows enhanced resistance to a chitin-binding fungus in transgenic tomato plants. Brazilian Journal of Medical Biology Research, 28: 743-750.

Lorito, M., Woo, S.L., Fernandez, G.I., Colucci, G., Harman, G.E., Pintor-Toro, J.A., Filione, E., Muccifora, S., Lawrence, C.B., Zoina, A. 1998. Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens. PNAS, 95: 7860-7865.

Massee, G. 1898. Tea Blights. Bulletin of Miscellaneous informations of the Royal Botanical Gardens Kew. 105-112.

Mauch, F., B. Mauch-Mani and T. Boller. 1988. Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinase and b-1,3-glucanase. Plant Physiology, 88: 936–940.

Mazau, D., D. Rumeau and M.T. Esquerre-Tugaye. 1987. Molecular aroaches to understanding cell surface interactions between plants and fungal pathogens. Plant Biochemistry, 25: 337-343.

Meena, B., Radhajeyalakshmi, R., Marimuthu, T., Vidhyasekaran, P., S. Doraisamy and R. Velazhahan. 2000. Induction of pathogenesis-related proteins, phenolics and phenylalanine ammonia lyase in groundnut by Pseudomonas fluorescens. Journal of Plant Disease and Protection, 107: 514–527.

Metraux, J.P., L. Streit and T. Staub. 1988. A pathogenesis-related protein in cucumber is a chitinase. Physiology and Molecular Plant Pathology, 33: 1-9.

Narayanasamy, P. 1980. Phenylalanine ammonia lyase and Tyrosine ammonia lyase in Alternaria alternata infected groundnut leaves. Indian Phytopathology, 33: 507-508.

Nasser, W, de Tapia, M., Kauffmann, S., S. Montasser-Kouhsari and G. Burkard. 1988. Identification and characterization of maize pathogenesis-related proteins. Four maize PR proteins are Chitinases. Plant Molecular Biology, 11: 529-538.

Pan, S.Q., X.S. Ye and J. Kuc. 1991. Association of ß -1, 3-glucanase activity and isoform pattern with systemic resistance to blue mold in tobacco-induced by stem injection with Perenospora tabacina of leaf inoculation with tobacco mosaic virus. Physiological and Molecular Plant Pathology, 39: 25-39.

Ponath, Y., Vollberg, H., K. Hahlbrock and E. Kombrink. 2000. Two differentially regulated class II chitinases from parsley. Biological Chemistry, 381(8): 667-678.

Punja, Z.K. and Y.Y. Zhang. 1993. Plant Chitinases and their roles in resistance to fungal diseases. Journal of Nematology, 25(4): 526-540.

Rajkumar, R., L. Manivel and S. Marimuthu. 1998. Logevity and factors influencing photosynthesis in tea leaves. Photosynthetica, 35: 41-46.

Ramamoorthy, V., T. Raguchander and R. Samiyaan, R. 2002. Enhancing resistance of tomato and hot peer to Pythium diseases by seed treatment with fluorescent pseudomonads. European Journal of Plant Pathology, 108: 429–441.

Reddy, M.N. and A.S. Rao. 1978. Physiology of host-parasite relations in damping off groundnut caused by Rhizoctonia solani. Journal of Phytopathology, 93: 193-207.

Ren, Y.Y. and C.A. West. 1992. Elicitation of Diterpene Biosynthesis in Rice (Oryza sativa L.) by Chitin. Plant Physiology, 99: 1169-1178.

Ryan, C.A. 1987. Oligosaccharide signalling in plants. Annual Review of Cell Biology, 3: 295-317.

Sadasivam, S. and A. Manickam. Biochemical methods. IInd Edition. New Age International (P) Limited, Publisher, Chennai.

Saikia, R., Singh, B.P., R. Kumar and D.K. Arora. 2005. Detection of pathogenesis-related proteins-chitinase and β-1, 3-glucanase in induced chickpea. Current Science, 89(4): 659-663.

Sathiyanathan, S. and P. Vidhyasekaran. 1981. Role of phenolics in brown spot disease resistance in rice. Indian Phytopathology, 34: 225-227.

Schlumbaum, A., Mauch, F., U. Vogeli and T. Boller. 1986. Plant chitinases are potent inhibitors of fungal growth. Nature, 324: 365–367.

Senthilkumar, P., K. Thirugnanasambantham and A.K.A. Mandal. 2012. "Suppressive subtractive hybridization aroach revealed differential expression of Hypersensitive response and reactive oxygen species production genes in tea (Camellia sinensis (L.) O. Kuntze) leaves during Pestalotiopsis thea infection". Applied Biochemistry and Biotechnology, 168(7): 1917-1927.

Shah, D.M. 1997. Genetic engineering for fungal and bacterial diseases. Current Opinion in Biotechnology, 8: 208-214.

Shewry, P.R. and J.A. Lucas. 1997. Plant proteins that confer resistance to pests and pathogens. Advanced Botanical Research, 26: 135-192.

Shoresh, M. and G.E. Gharman. 2010. Differential expression of maize chitinases in the presence or absence of Trichoderma harzianum strain T22 and indications of a novel exo- endo- heterodimeric chitinase activity. BMC Plant Biology, 10: 136.

Staskawicz, B. J., Ausubel, F. M., Baker, B. J., J.G. Ellis and J. D. G. Jones. 1995. Molecular genetics of plant disease resistance. Science, 268: 661–667.

Vidhyasekaran, P. and R. Velazhahan. 1996. Elicitor induced defence responses in suspension cultured rice cells, In: Chopra, V. C., R.P. Sharma and M.S. Swaminathan (Eds). Agricultural biotechnology. Oxford & IBH Publishing Co., New Delhi. 249-255.

Wan, J., X. Zhang and G. Stacey. 2008. Chitin signalling and plant disease resistance. Plant Signalling and Behaviour, 3(10): 831-833.

Ward, E.R., Uknes, S.J., Williams, S.C., Dincher, S.S., Wierhold, D.L., Alexander, D.C., Ahl-Goy, P., J.P. Metraux and J.A. Ryals. 1991. Coordinate gene activity in response to agents that induce systemic acquired resistance, Plant Cell, 3: 1085-1094.

Zdor, R.E. and A.J. Anderson. 1992. Influence of root colonizing bacteria on the defense responses in bean. Plant and Soil, 140: 99–107.


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