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DIFFERENTIAL EXPRESSION OF PATHOGENESIS-RELATED PROTEINS DETERMINES THE DISEASE TOLERANCE AND SENSITIVITY IN TEA CULTIVARS DURING GREY BLIGHT DISEASE DEVELOPMENT | Palanisamy | Pakistan Journal of Phytopathology

DIFFERENTIAL EXPRESSION OF PATHOGENESIS-RELATED PROTEINS DETERMINES THE DISEASE TOLERANCE AND SENSITIVITY IN TEA CULTIVARS DURING GREY BLIGHT DISEASE DEVELOPMENT

Senthilkumar Palanisamy, Suganthi Muthusamy, A.K.A Mandal

Abstract


Tea is one among the non-alcoholic beverages consumed by people across the continents. As tea is a monocrop, it serves a haven for numerous pests and pathogens. Being a timeless plant, the agronomical practices and stable microclimate favours the disease development. Among the diseases affecting the leaves, grey blight – courtesy of Pestalotiopsis theae- is one of the most vital and detrimental diseases in tea. Plant modifies its arsenal system against the pathogens to produce higher levels of antimicrobial products which includes pathogenesis proteins. Understanding the expression pattern of these proteins will help in developing disease tolerance cultivars. All the three pathogenesis-related proteins exhibited higher expression in the impaired cultivars, while the sensitive cultivars showed lesser degree of enzyme concentration. Expression pattern of pathogenesis-related proteins like Phenylalanine ammonia-lyase, β-1,3 glucanases and chitinase were reported and discussed greatly.Activity of all three enzymes were found to be increased in resistant cultivars than the sensitive cultivars. The enzyme activity was slightly higher in the infected plants when compared with the uninfected plants.


Keywords


Pestalotiopsis theae; Camellia sinensis; Pathogenesis related proteins; grey blight

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