Mamoona Hassan, Naureen Akhtar, Muhammad Riaz, Salik N. Khan, Muhammad Shakeel, Ateeq Tahir


The application of various strategies is being used to manage the Plant diseases.  These strategies are used for the management of plant diseases by applying the cultural, genetical, chemical and biological controls. The biological control approaches are getting more importance on account of their less expensive and environmental friendly usage.  Therefore, the research is conducted to evaluate the anti-microbial activity of Nigella sativa seed oil for the management of the most common plant pathogenic gram -ve bacteria pseudomonas and burkholderia.  The isolated bacterias are being mentioned here with FCBP reference like Pseudomonas syringae pv syringae (FCBP009), P. Syringae pv populans (FCBP010), P. Syringae (FCBP405), Burkholderia pseudomallei (FCBP036; FCBP350; FCBP460) and B. Glumae (FCBP459) were collected from infected fruit/seed. The above mentioned bacterial strains were examined by using concentrations (10, 40, 60, 100 and 200 ppm) of Nigella sativa seed oil, ampicillin and streptomycin antibiotics. After investigation, the study found that Nigella sativa maximum inhibition (65.0 mm) were recorded at 200 ppm concentration in assessment of B. Glumae (FCBP459) pathogen. Conversely, the minimum inhibition (20.25 mm) were recorded at the similar concentration in assessment of P. syringae pv. syringae (FCBP405). Particularly, the present research observes that the antibiotic drugs use against the above mentioned bacteria have shown the great variation in resistance. This research suggests to use of antibacterial materials from biological origin in managing these disease-causing bacteria.


Antibiotics, black seed oil, characterization, enzymatic activity, phytopathogens.

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Pakistan Journal of Phytopathology
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