NOVEL REPORT OF ROSA INDICA LEAF SPOT BY ALTERNARIA TENUSSIMA FROM PAKISTAN

Sobiya Shafique, Rubab Rafiq, Shazia Shafique, Abrar Hussain, Alina Javed, Ayesha Mubarak

Abstract


Fungal leaf spots disease is a major threat to Rosa indica in Pakistan. To meet the required demands of rose, large scale production of roses without any fungal disease is necessary. For this purpose, isolation and identification of pathogen from rose plant was carried out by observing phenotypic characters. Further detailed confirmation of isolated pathogen was executed on molecular bases using ITS, EF and GAPDH primers. On these bases, fungal pathogen was confirmed as Alternaria tenussima. Then, Koch’s Pathogenicity test was applied to ratify the virulence level of novel isolated fungal pathogen by artificially inoculating it on R. indica seedlings in plate and pot trials.


Keywords


Alternaria tenussima, Identification, leaf spot, pathogenicity, Rosa indica.

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References


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DOI: https://doi.org/10.33866/phytopathol.033.01.0620

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Copyright (c) 2021 Sobiya Shafique, Rubab Rafiq, Shazia Shafique, Abrar Hussain, Alina Javed, Ayesha Mubarak

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Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.