Saher Ashraf, Ghulam Mustafa, Muhammad S. Khan, Muhammad Aslam


Potato is the 4th major vegetable crop in our country but more than 90% of the seed is imported which costs heavy economic burden on the foreign exchange. Further, imported germplasm is not well adapted to local climatic conditions and is vulnerable to different types of diseases. In vitro manipulations have proved their worth not only for the mass multiplication but also for the production of disease free seed of various crop plants. A proficient in vitro micropropagation and tuberization system was established in elite potato cultivars Kuroda and Desiree. In addition to growth hormones, sucrose concentration and light regime appeared to have dominant effect on microtuberization. Maximum number of microtubers was observed in genotype Desiree (2.20±0.20) followed by Kuroda (2.0±0.316) on MS medium augmented with 8% sucrose. Average fresh weight was maximum (0.70±0.063 g) on MS medium augmented with 10% sucrose in genotype Desiree. Likewise, best microtuberization was observed in initial 20 days light (16 hrs light with 8 hrs dark) incubation followed by complete dark incubation. The resultant microtubers were multiplied in pots for the production of minitubers and were tested for disease infestation by ELISA (Enzyme Linked Immunosorbant Assay). The antigen-antibody interaction was detected to be similar to the negative control (0.35-0.528) indicating absence of the viral pathogens. Hence, the developed protocol can be employed for the mass multiplication of disease free seed of elite potato cultivars resulting in reduced dependence on imported seed.


Microtubers; Solanum Tuberosum L; Light regimes; Sucrose

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Ahmed, N., A. Khan, A. Khan and A. Ali. 2015. Prediction of potato late blight disease based upon environmental factors in Faisalabad, Pakistan. Journal of Plant Pathology and Microbiology, 1:1: 1-6.

Alisdair, R.F. and L. Willmitzer. 2001. Molecular and biochemical triggers of potato tuber development. Plant Physiology, 127: 1459-1465.

Ammar, M., S. Hameed, I. Haque, S. M. Mughal, H. Raiz, R. Parveen and G. Yasmin. 2013. Serological identification of Citrus Tristeza Closterovirus (CTV) and molecular characterization of its coat protein gene. Pakistan Journal of Phytopathology, 25(1): 23-26.

GOP. 2020-2021. Pakistan Economic Survey. Economic Advisor’s Wing, Finance Division, Government of Pakistan, Islamabad.

Aslam, A., A. Ali, N. H. Naveed, A. Saleem and J. Iqbal. 2011. Effect of interaction of 6- benzyl aminopurine (BA) and sucrose for efficient microtuberization of two elite potato (Solanum tuberosum L.) cultivars, Desiree and Cardinal. African Journal of Biotechnology, 10(59): 12738-12744.

Badoni, A. and J.S. Chauhan, 2010. Importance of potato microtuber seed material for farmers of Uttarakhand hills. International Journal of Sustainable Agriculture, 2(1): 01-09.

Batool, A., M. A. Khan, J. Farooq, S. M. Mughal and Y. Iftikhar. 2011. ELISA screening of potato germplasm against potato leaf roll virus. Journal of Agriculture Research, 49(1): 57-63.

Clark, M. F. and A.W. Adams. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay (ELISA) for detection of plant viruses. Journal of Genernal Virology, 34: 475-483.

Dobranszki, J. and M. Mandis. 1993. Induction of in vitro tuberization by short day period and dark treatment of potato shoot grown on hormone free medium. Acta Biologica Hungarica, 44: 411-420.

Dobranszki. J. 2001. Effects of light on in vitro tuberization of the potato cultivar Desiree and its relatives. Acta Biologica. Hungarica, 52(1): 137-147.

Donnelly, J. D., W. K. Coleman and S. E. Coleman. 2003. Potato microtuber production and performance: A review. American Journal of Potato Research, 80: 103-115.

Ebadi, M. and A. Iranbakhsh, 2011. The induction and growth of potato (Solanum tuberosum L.) microtubers (sante cultivar) in response to the different concentration of 6-benzylaminopurine and sucrose. African Journal of Biotechnology, 10(52): 10626-10635.

Fatima, B., M. Usman, I. Ahmad and I. A. Khan. 2005. Effect of explant and sucrose on microtuber induction in potato cultivars. International Journal of Agriculture and Biology, 7(1): 63-66.

Fufa, M., and M. Diro, 2014. Mictotubers induction of two Potato (Solanum tuberosum L.) varieties. Advances in Crop Science and Technology. 2(2):122-125.

Gami, R.A., S. K. Parmar, P. T. Patel, C. J. Tank, R. M. Chauhan, H. S. Bhadauria and S. D. Solanki. 2013. Microtuberization, minitubers formation and in vitro shoot regeneration from bud sprouts of potato (Solanum tuberosum L.) cultivar K. badshah. African Journal of Biotechnology, 12(38): 5640-5647.

Hameed, A., Z. Iqbal, S. Asad and S. Mansoor. 2014. Detection of Multiple potato viruses in the field suggests synergistic interactions among potato viruses in Pakistan. The Plant Pathology Journal, 30(4): 407-415.

Hoque, M. E. 2010. In vitro tuberization in potato (Solanum tuberosum L.). Plant Omics Journal, 3(1):7-11.

Hossain, M. A., M. A. Kawochar, A. Al-Mahmud, E. H. M. S. Rahaman, M. A. Hossain and K. M. Nasiruddin. 2015. Standardization of sucrose and 6-benzyl aminopurine for in vitro microtuberization of potato. American Journal of Agriculture and Forestry, 3: 25-30.

Hossain, M. S., M. M. Hossain, T. Hossain, M. M. Haque, M. Zakaria and M. D. Sarkar. 2017. Varietal performance of potato on induction and development of microtuber in response to sucrose. Annals of Agricultural Sciences, 62: 75-81.

Hussain, I., Z. Chaudhry, A. Muhammad, R. Asghar, S. M. S. Naqvi and H. Rashid. 2006. Effect of chlorocholine chloride, sucrose and BAP on in vitro tuberization in potato (Solanum tuberosum L. Cardinal). Pakistan Journal of Botany, 38(2): 275-282.

Iftikhar, Y., M. A. Zeshan, M. U. Ghani, A. Ali, S. Saleem, T. A. Hamid and T. Mahmood. 2021. Infectivity assays for soyabean and cowpea Mosaic viruses and their management. Pakistan Journal of Phytopathology, 33(02): 283-292.

Imani, A. A., R. Qhrmanzadeh, J. Azimi, and J. Jampoor. 2010. The effect of various concentration of 6-Benzylaminopurine (BAP) and sucrose on in vitro potato (Solanum tuberosum L.) microtuber induction. American-Eurasian Journal of Agricultural and Environmental Sciences, 8(4): 457-459.

Jackson. S.D. 1999. Multiple signaling pathways control tuber induction in potato. Plant Physiology, 119: 1-8.

Kanwal, A., A. Ali and K. Shoaib. 2006. In vitro microtuberization of potato (Solanum tuberosum L.) cultivar Kuroda- A new variety in Pakistan. International Journal of Agriculture and Biology, 8(3): 337-340.

Khuri, S. and J. Moorby, 1995. Investigation into the role of sucrose in potato cv. Estima microtuber production in vitro. Annals of Botany, 75: 295–303.

Kumlay, A. M., N. Arslan and C. Kaya. 2014. Factors affecting microtuberization of potato (Solanum tuberosum L.) on in vitro conditions. Anadolu Journal of Agricultural Sciences, 29(2):154-165.

Mamiya, K., K. Tanabe and N. Onishi. 2020. Production of potato (Solanum tuberosum, L.,) microtubers using plastic culture bags. Plant Biotechnology, 37: 233-238.

Naqvi, B., H. Abbas and H. Ali. 2019. Evaluation of in vitro tuber induction ability of two potato genotypes. Pakistan Journal of Agricultural Sciences, 56(1): 77-81.

Raza, W., M. U. Ghazanfar and M. I. Hamid. 2019. Occurrence of late blight (Phytophthora infestans (Mont.) de Bary) in major potato growing areas of Punjab, Pakistan. Sarhad Journal of Agriculture, 35(3):806-815.

Samant, A., V. A. Kumar, A. Kumar, P. S. Shukla and K. Joshi. 2018. In vitro microtuber production in potato cultivar kufri himalini. Advances in Plants Agriculture Research, 8(6): 648-653.

Samsatly, J., M. Jawhari, C. Najjar, H. Sobh, and Y. Abou-Jawdah. 2014. Modification of serological techniques and their evaluation for detection of potato viruses in seed certification related activities. Crop Protection, 61: 51-57.

Spooner, D. M., 2013. Solanum tuberosum (potatoes), 2nd ed. In: Maloy, S., and K. Hughes, (Ed.), Brenner’s Encycyclopedia of Genetics, Academic Press, 481-483.

Wang, P.J., C.Y. Hu. 1985. Potato tissue culture and its application. In: Li, P.H. (Ed.), Potato Physiology. Academic Press, Orlando, 503- 577.

DOI: https://doi.org/10.33866/phytopathol.034.01.0742


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Pakistan Journal of Phytopathology
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