Medjahed Khaldia, Belkahla Hadjira, Abdelaziz Merouane


This study was designed to survey Barley Stripe Mosaic Virus (BSMV) in various cereal varieties cultivated in 28 fields cultivated in seven districts namely Boukadir, Sobha, Ouled Fares, Tadjena, Kaloul, Abou Elhacene and El Karimia located in Chlef province, North West of Algeria. The survey covered 2 barley, 2 durum wheat, 4 common wheat and one oat varieties and conducted from February to the May 2017. The main objectives of this investigation were to determine the incidence of Barley Stripe Mosaic Virus in crop cereals cultivated in Algeria as well as to verify the resistant varieties. The Barley Stripe Mosaic Virus was examined in plants and seeds symptomatically by mechanical inoculation and serologically by TAS-ELISA technique using specific monoclonal antibodies. The symptoms surveying noticed more expression in spike cereals. The results of serological analysis revealed infection of 27 fields that present 96.24%. The mechanical inoculation test proved infection of totality of seed lots and seeds transmission test showed efficiency of seed transmission with a rate of 77%. In terms of species susceptibility, barley was the most susceptible crop followed by durum wheat and common wheat, whereas the oat was not infected. This investigation is the first report on Barley Stripe Mosaic Virus naturally infecting cereals in the cereal producer zone of Chlef. The findings provide useful information on viral infection of cereal fields, thus, as a priority measurement there is a need to reinforce control of cereal seeds.


BSMV; Hordeїvirus; Cereals; Transmission; TAS-ELISA

Full Text:



Abd El-Aziz, M. H. 2019. Three modern serological methods to detect plant viruses. Journal of Plant Science and Phytopathology, 3: 101-106.

Adams, M. J., E. Lefkowitz and E. B. Carstens. 2011. Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses. Elsevier.

Belkahla, H. and H. Lapierre. 2005. Serodetection of viruses associated to barley yellow dwarf (BYD) on cereals in Algeria. Phytoprotection, 80: 169-177.

Carroll, T. W. 1980. Barley Stripe Mosaic Virus: Its Economic Importance and Control. Plant Disease, 64: 136.

CGKB. 2022. Viruses-barley: Barley Stripe Mosaic Virus (BSMV). Crop Genebank Knowledge Base, Montpellier, France.

Chiko, A. W. 1975. Natural occurrence of barley stripe mosaic virus in wild oats (Avena fatua). Canadian Journal of Botany, 53: 417-420.

EPPO. 1983. Barley Stripe Mosaic Virus. EPPO Bulletin, 13: 1-5.

Hafez, E. E., E. E. A. Aleemand F. A. Fattouh. 2008. Comparison of Barley Stripe Mosaic Virus Strains. Zeitschriftfür Naturforschung, 63: 271-276.

Herrera, M., M. Edmundo Beratto, V. Orlando Andrade and V. Monica Madariaga. 2001. Identification of barley stripe mosaic virus (BSMV) on barley in Chile. Agricultura Tecnica, 61: 275-280.

Ilbağı, H., F. Rabenstein, A. Habekuss, F. Ordonand A. Çıtır. 2007. Incidence of virus diseases in maize fields in the Trakya region of Turkey. Phytoprotection, 87: 115-122.

Jackson, A. O., H.-S. Lim, J. Bragg, U. Ganesanand M. Y. Lee. 2009. Hordeivirus Replication, Movement, and Pathogenesis. Annual Review of Phytopathology, 47: 385-422.

Jezewska, M. 2001. Identification of Barley stripe mosaic virus in Poland. Journal of Plant Protection Research, 41(2): 164-167.

Jeżewska, M. and K. Trzmiel. 2009. Impact of Seed-Transmitted Viruses on Quality of Cereal Seeds. Journal of Plant Protection Research, 49: 460-464.

Jones, R. A. C., M. Sharman, P. Trębicki, S. Mainaand B. S. Congdon. 2021. Virus Diseases of Cereal and Oilseed Crops in Australia: Current Position and Future Challenges. Viruses, 13(10): 2051.

Kapooria, R. G. and J. Ndunguru. 2004. Occurrence of viruses in irrigated wheat in Zambia. EPPO Bulletin, 34: 413-419.

Kılıç, H., D. Altındal, N. Yardımcı and L. Akgün. 2012. Detection of Wheat streak mosaic virus and Barley stripe mosaic virus by DAS-ELISA methods in seeds of different wheat cultivars. Ziraat Fakültesi Dergisi, Uludağ Üniversitesi, 26: 17-25.

Köklü, G. 2004. Occurrence of cereal viruses on wheat in Tekirdag, Turkey. Phytoprotection, 85: 19-25.

Lapierre, H. D. and Hariri, D. 2008. Cereal viruses: wheat and barley, In: B. W. J. Mahy & M. H. V. Van Regenmortel, (eds.), Desk encyclopaedia of plant and fungal virology. Academic Press, London, UK.

Lee, W.-S., K. E. Hammond-Kosackand K. Kanyuka. 2012. Barley stripe mosaic virus-mediated tools for investigating gene function in cereal plants and their pathogens: virus-induced gene silencing, host-mediated gene silencing, and virus-mediated overexpression of heterologous protein. Plant Physiology, 160: 582-590.

Lim, C. H., I. Y. Mohammed, Y. A. Abakr, F. K. Kazi, S. Yusup and H. L. Lam. 2016. Novel input-output prediction approach for biomass pyrolysis. Journal of Cleaner Production, 136: 51-61.

Lommel, S. A. 1986. Identification of Wheat Spindle Streak Mosaic Virus and Its Role in a New Disease of Winter Wheat in Kansas. Plant Disease, 70: 964.

Nutter, D. O. 1984. Access to Care and the Evolution of Corporate, for-Profit Medicine. New England Journal of Medicine, 311: 917-919.

Sastry, K. S. 2013. Seed-borne plant virus diseases. Springer, New Delhi, India.

Seepiban, C., S, Charoenvilaisiri, N. Warin, A. Bhunchoth, N. Phironrit, B. Phuangrat, O. Chatchawankanphanich, S. Attathom and O. Gajanandana. 2017. Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus. Virology Journal, 14(1): 1-14.

Wiese, M. 1987. Compendium of wheat diseases. APS Press, St. Paul, MN. Compendium of wheat diseases. 2nd ed. APS Press, St. Paul, MN, United States.

Younes, H., D. Aseel and M. Abd El-Aziz. 2018. Identification and purification of cowpea mosaic comovirus isolated from infected cowpea (Vigna unguiculata L.) in northern Egypt. International Journal of Agricultural Environment and Research, 4: 20.

Zarzyńska-Nowak, A., B. Hasiów-Jaroszewskaand M. Jeżewska. 2018. Molecular analysis of barley stripe mosaic virus isolates differing in their biological properties and the development of reverse transcription loop-mediated isothermal amplification assays for their detection. Archives of Virology, 163: 1163-1170.



  • There are currently no refbacks.

Copyright (c) 2022 Abdelaziz MEROUANE

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.