In vitro Antibacterial Activity of Tropical Herbs Plant Combined with Silage Extract Against Pathogen Bacteria: Initial Screening
DOI:
https://doi.org/10.33866/phytopathol.037.02.1042Keywords:
Antioxidant Capacity, Leaves, Pathogen Bacteria, PhytochemicalAbstract
This study aims to investigate the effect of using various herbal plants in combination with fermentation treatment of silage on the inhibition of pathogenic bacteria. The research consisted of two main phases. The first phase included measuring the qualitative phytochemical profile and determining the lactic acid bacteria content of 7 different types of silage extract. The second phase involves quantitative phytochemical profiles and antioxidant capacity against pathogenic bacteria. Descriptive analysis method was used in this research. The research results showed that silage extract from Dracaena fragrans, Tithonia diversifolia, and Acanthus ilicifolius L leaves was the main choice because of its abundant lactic acid bacteria and phytochemical content. Dracaena fragrans silage extract, which contains flavonoids and tannins in amounts of 0.02 and 0.01 ml/100 mg, together with antioxidants of 926 ppm, is able to produce antibacterial compounds with a strong category for inhibiting pathogenic bacteria. Thus, it can be concluded that the use of Dracaena fragrans-based silage extract is able to provide strong inhibition against pathogenic bacteria based on its phytochemical, lactic acid bacteria and antioxidant content.References
Aulifa D. L., S. N. Fitriansyah, S. A. Ardiansyah, D. P. Wibowo, Y. A. Julata, and D. S. Christy. 2017. Phytochemical Screening, Antibacterial Activity, and Mode of Action on Morus nigra. Pharmacognosy Journal, Volume 10 Article 1, 167–171. https://doi.org/10.5530/pj.2018.1.28
Chaudhary S, A. Negi, and V. Dahiya. 2010. The study of in vitro antimicrobial activity and phytochemical analysis of some medicinal plants in Chamoli Garhwal Region. Pharmacognosy Journal, Volume 2 Article 12, 481–485. https://doi.org/10.1016/s0975-3575(10)80035-5
Ernawati, E. Suprayitno, Hardoko and U. Yanuhar. 2019. Extraction of bioactive compounds fruit from Rhizophora mucronata using sonication method. IOP Conference Series: Earth and Environmental Science, Volume 236 Article 1. https://doi.org/10.1088/1755-1315/236/1/012122
Hur, S. J., S. Y. Lee, Y. C. Kim, I. Choi, and G. B. Kim. 2014. Effect of fermentation on the antioxidant activity in plant-based foods. Food Chemistry, Volume 160, 346–356. https://doi.org/10.1016/j.foodchem.2014.03.112
Khumaida, N, M. Syukur, M. Bintang, and W. Nurcholis. 2019. Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in west java, Indonesia. Biodiversitas, Volume 20 Article 3, 656–663. https://doi.org/10.13057/biodiv/d200306
Kumar, A, P. Nirmal, M. Kumar, A. Jose, V. Tomer, E. Oz, C. Proestos, M. Zeng, T. Elobeid, V. Sneha and F. Oz. 2023. Major Phytochemicals: Recent Advances in Health Benefits and Extraction Method. In Molecules, Volume 28 Article 2. MDPI. https://doi.org/10.3390/molecules28020887
Morales G, A. Paredes, L. A. Loyola and J. Borquez. 2003. Secondary metabolites from four medicinal plants from northern Chile : Antimicrobial activity and biotoxicity against Artemia salina. Journal of the Chilean Chemical Society, June 2014. https://doi.org/10.4067/S0717-97072003000200002
Prihambodo, T. R., M. M. Sholikin, N. Qomariyah, A. Jayanegara, I. Batubara, D. B. Utomo, Nahrowi. 2021. Effects of dietary flavonoids on performance, blood constituents, carcass composition and small intestinal morphology of broilers: a meta-analysis. Animal Bioscience, Volume 34 Article 3. https://doi.org/10.5713/ajas.20.0379
Ramli, N., and A. Setiyono. 2015. Karasteristik jus dari silase jagung berbeda umur serta kemampuannya dalam menghambat e . Coli dan salmonella sp ( Juice characteristics of corn silage from different age and its cap ... February.
Salim Z. 2017. Info komoditi I.
Shang, Y. F., H. Cao, Y. L. Ma, C. Zhang, F. Ma, C. X. Wang, X. L. Ni, W. J. Lee and Z. J. Wei. 2019. Effect of lactic acid bacteria fermentation on tannins removal in Xuan Mugua fruits. Food Chemistry, Volume 274 118–122. https://doi.org/10.1016/j.foodchem.2018.08.120
Svensson L, B. Sekwati-Monang, D. L. Lutz, R. Schieber, and M. G, Gänzle. 2010. Phenolic acids and flavonoids in nonfermented and fermented red sorghum (Sorghum bicolor (L.) Moench). Journal of Agricultural and Food Chemistry, Volume 58 Article 16, 9214–9220. https://doi.org/10.1021/jf101504v
Tian J, R. Na, Z. Yu, Z. Liu, and Y. Yu. 2018. Inoculant effects on the fermentation quality, chemical composition and saponin content of lucerne silage in a mixture with wheat bran or corn husk. Animal Production Science, Volume 58 Article 12, 2249. https://doi.org/10.1071/an16407
Wijayanti E, and N. Setiawan. 2017. The effect of lactic acid fermentation on fig (Ficus carica) fruit flavonoid. Journal of Biological Research, 23(1), 39–44.
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