Impact of Abiotic Factors on In Vitro Development of Aspergillus flavus Isolated from Sesame Seeds
DOI:
https://doi.org/10.33866/phytopathol.036.02.1181Keywords:
Aspergillus flavus, pH, temperature, light, carbon source, nitrogen source.Abstract
Plant pathogen’s growth and development are influenced by abiotic conditions, which impact sesame crop production. Aspergillus flavus was selected for this research based on its prevalence and disease incidence in sesame. Under in vitro conditions, the development of A.flavus was investigated at various temperatures, pH levels, light intensity, nitrogen, and carbon sources. The data was collected after 3, 7, and 10 days of incubation on potato dextrose agar (PDA). The best sporulation was observed at 25°C, with a colony diameter of 3.5 cm. Mycelia development of 1.1 cm and an uneven colony border were observed during a continuous dark phase. A. flavus could develop moderate sporulation at 5.0 pH with colony diameter of 2.2 cm. Among carbon sources, sucrose promoted maximal mycelial growth of 1.55 cm with the best sporulation. Sodium nitrate, as a nitrogen source, also showed a maximum diameter of 4.1cm. The findings of this study showed certain levels of abiotic factors that promote the development of A. flavus. By avoiding these abiotic factors, farmers and researchers can increase the quantity and quality of sesame crop production.References
Arun, A. T. and S. Mahabeer. 2023. Seed Mycoflora of Sesame (Sesamum indicum L.) and their Phytopathogenic Effect. International Journal of Current Microbiology and Applied Sciences, 12(08): 49-67.
Bais, R. K., V. Ratan and S. K. Somesh. 2019. Influence of temperature, pH and light on growth and sporulation of Alternaria solani (Ellis and Martin) Jones and Grout causing early blight of tomato (Solanum lycopersicum L.) under in vitro condition. International Journal of Current Microbiology and Applied Sciences, 8(12): 36-46.
Gebremeskel, A. F., N. P. Ngoda., W. E. Kamau‐Mbuthia and M. S. Mahungu. 2022. The effect of roasting, storage temperature, and ethanoic basil (Ocimum basilicum L.) extract on the oxidative stability of crude sesame (Sesamum indicum L.) oil. Food Science and Nutrition, 10(8): 2736-2748.
Hathout, A.S. and S.E. Aly. 2014. Biological detoxification of mycotoxins: A review. Annals of Microbiology, 64(3): 905-919.
Hubballi, M., S. Nakkeeran., T. Raguchander., T. Anand and R. Samiyappan. 2010. Effect of environmental conditions on growth of Alternaria alternata causing leaf blight of noni. World Journal of Agricultural Sciences, 6(2): 171-177.
Jayaswal, R. K., R. Singh and S. Y. Lee. 2003. Influence of physiological and environmental factors on growth and sporulation of an antagonistic strain of Trichoderma viride RSR 7. Mycobiology, 31(1): 36-41.
Kahyaoglu, T. and Kaya, S. 2006. Modeling of moisture, color and texture changes in sesame seeds during the conventional roasting. Journal of Food Engineering, 75(2): 167-177.
Klich, M. A. 2007. Aspergillus flavus: the major producer of aflatoxin. Molecular plant pathology, 8(6): 713-722.
Kumar, P., K. D. Mahato., M. Kamle., K. T. Mohanta and G. S. Kang. 2017. Aflatoxins: A global concern for food safety, human health and their management. Frontiers in microbiology, 7: 2170.
Kumar, V., P. K. Kumar and M. K. Kumar. 2015. Study of variability and sporulation by isolates of Alternaria solani of Lycopersicon esculentum (Mill.). Asian Journal of Science and Technology, 6: 1264-1270.
Mariana, M., S. Abbas, S. Salamiah, Y. Marsuni, S. Soedijo, M. Sepe, M.I. Pramudi, I.S. Budi, D. Fitriyanti, E. Liestiany and L. Aphrodyanti. 2023. In Silico analysis of Aspergillus flavus fungal proteins: structural and functional insights using its primers. Pakistan Journal of Phytopathology, 35(2): 397-408.
Mobeen, A.K., A. Aftab., A. Asif and S. A. Zuzzer. 2011. Aflatoxins B1 and B2 contamination of peanut and peanut products and subsequent microwave detoxification. Journal of Pharmacy and Nutrition Sciences, 1(1): 1-3.
Probst, C., H. Njapau and J. P. Cotty. 2007. The outbreak of an acute aflatoxicosis in Kenya in 2004: identification of the causal agent. Applied and environmental microbiology, 73(8): 2762-2764.
Quasem, J. M., S. A. Mazahreh and K. Abu-Alruz. 2009. Development of vegetable-based milk from decorticated sesame (Sesamum indicum). American Journal of Applied Sciences, 6(5): 888.
Rajput, A. Q., & Shahzad, S. 2015. Growth and sporulation of Trichoderma polysporum on organic substrates by addition of carbon and nitrogen sources. Pakistan Journal of Botany, 47(3): 979-986.
Usman, M., M. Atiq, N.A. Rajput, S.T. Sahi, M. Shad, N. Lili, S. Iqbal, A.M. Arif, U. Ahmad, K.S. Khan, M. Asif, F.U. Haider. 2023. Efficacy of Green Synthesized Silver Based Nanomaterials against Early Blight of Tomato Caused by Alternaria solani. Gesunde Pflanzen. 1-11.
Usman, M., M. Razzaq., R. A. R. Khan., A. M. Rehman., M. M. Ali., S. Gull and S. K. Golokhvast. 2022. Factors Affecting Postharvest Losses of Sesame (Sesamum indicum L.) and Their Mitigation Strategies. Agronomy, 12(10): 2470.
Williams, W. P. 2006. Breeding for resistance to aflatoxin accumulation in maize. Mycotoxin research, 22 (1): 27-32.
Yaqoob, F., M. Atiq, N.A. Rajput, A. Nawaz, M. Kashif, M.J. Matloob, A. Jabbar, W. Din, F. Ali and A. Ullah. 2024. Appraisement of chemotherapy, plant defense activators, and genetic resistance against eyespot disease in sugarcane. Plant protection, 08(02): 325-340.
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