ANTAGONISTIC POTENTIAL OF TRICHODERMA HARZIANUM AGAINST FUSARIUM OXYSPORUM F. SP. CUBENSE ASSOCIATED WITH PANAMA WILT OF BANANA

Babar Khan, Zuniara Akash, Shahzad Asad, Nazir Javed, Nasir A. Rajput, Abdul Jabbar, Wasi U. Din, Rana M. Atif

Abstract


Fusarium oxysporum f. sp. cubense is a soil loving pathogen of banana that can cause distortion of vascular system. It is responsible for the disease Fusarium wilt of bananas also renowned as panama wilt disease that has responsible of immense losses in the banana industry worldwide. In this study, diseased samples were collected from rhizosphere of banana plants in the research area of National Agriculture Research Centre (NARC), Islamabad and cultured on PDA to isolate pathogenic strains of F. oxysporium f. sp. cubense. Antagonistic potential of Trichoderma harzianum against F. oxysporum f. sp. cubense was evaluated under controlled conditions. T. harzianum produced up to 75.5% inhibition of colony growth of the pathogen followed by incubation for 72 h at 28+2°C in vitro. In pot culture T. harzianum considerably reduced disease severity. It proves that it is a potential biological control agent against banana wilt pathogen.


Keywords


Keywords: Fusarium wilt, Inhibition, Pot culture, Biological control

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References


Agrios, G.N. 2005. Plant Pathology (Vol. 5). Burlington, MA: Elsevier Academic Press.

Ahmed, Z.M., S. Dawar and M. Tariq. 2009. Fungicidal potential of some local tree seeds for controlling root rot disease. Pak. J. Bot. 41(3): 1439-1444.

Albert, S., D. Chauhan, B. Pandya and A. Padhiar. 2011. Screening of Trichoderma spp. as potential fungal partner in co-culturing with white rot fungi for efficient bio-pulping. Glob. J. Biotechnol. Biochem. 95-101.

Ambikapathy, V. 2000. Studies on the saprophytic behaviors and suppression of Rhizoctonia solani kuhn, a broad-spectrum pathogen, Ph.D. Thesis, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.

Amsellem, Z., N.K. Zidack, P.C. Quimby and J. Gressel. 1999. Long-term dry preservation of viable mycelia of two mycoherbicidal organisms. Crop Prot. 18: 643–649.

Anania, G. 2006. The 2005 WTO arbitration and the new EU import regime for bananas: a cut too far? Eur. Rev. Agric. Econ. 33(4): 449-484.

Barnett, H.L. and B.B. Hunter 1972."Illustrated genera of imperfect fungi."(3rd Edn).

Benhamou, N. and I. Chet. 1993. Hyphal interactions between Trichoderma harzianum and Rhizoctonia solani: Ultrastructure and gold cytochemistry of the mycoparasitic process. Phytopathol. 83: 1062-1071

Bentley, P.J. 1998. Comparative vertebrate endocrinology. Cambridge University Press.

Brunner K, C.K. Peterbauer, R.L. Mach, M. Lorito, S. Zeilinger, R.L.Kubicek. 2003. The N-acetylglucosaminidase of Trichoderma atroviride is essential for chitinase induction by chitin and major relevance to bio-control. Curr. Gen. 43: 289-295.

Esposito, R.G. and A.M. Fletcher. 1961. The relationship of pteridine biosynthesis to the action of copper 8-hydroxyquinolate on fungal spores. Arch. Biochem. Biophy. 93(2): 369-376.

FAO. 2011. FAO Stat Database Collection http://apps.fao.org/ page/collection (accessed on 17-06-2015).

Harman, G.E. and C.P. Kubicek. 1998. Trichoderma and Gliocladium. Enzymes, biological control and commercial applications. Taylor & Francis, London. 2:393.

Harman, G.E., C.R. Howell, A. Viterbo, I. Chet and M. Lorito. 2004. Trichoderma species opportunistic, avirulent plant symbionts. Nat. Rev. Microbiol. 2: 43-56.

Howell, C.R. 1998. The role of antibiosis in biocontrol. In Trichoderma and Gliocladium, Ed. C. P. Kubicek and G. E. Harman. London, Bristol, PA: Taylor & Francis. 173-184.

Kucuk, C and M. Kivan. 2004. In vitro antifungal activity of strains of Trichoderma harzianum. Turk. J. Biol. 28: 111-115.

Kullnig, C., R.L. Mach, M. Lorito and C.P. Kubicek. 2000. Enzyme diffusion from Trichoderma atroviride to Rhizoctonia solani is a prerequisite for triggering of Trichodermae ech42 gene expression before mycoparasitic contact. Appl. Environ. Microbiol. 66: 2232-2234.

Leslie, J.F., B.A. Summerell and S. Bullock. 2006. The Fusarium laboratory manual. Vol. 2, No. 10. Ames, IA, USA: Blackwell Pub.

Lorito, M., S.L. Woo, E. Gary, G.E. Harman and E. Monte. 2010. Translational research on Trichoderma: From 'Omics to the Field. Ann. Rev. Phytopathol. 48: 395-417.

Lorito, M., S.L. Woo, M. D. Ambrosio, G.E. Harman, C.K. Hayes, C.P. Kubicek and F. Scala. 1996. Synergistic interaction between cell wall degrading enzymes and membrane affecting compounds. Mol. Plant Microb. Interact. 9: 206-213.

Molina, A.B. and R.V. Valmayor. 1999. Banana production systems in South East Asia. Bananas and Food security, Pica C., E. Foure, EA Frison (Eds.), INIBAP, Montpellier, France. 423-436.

Muthukumar, C., R. Vijayakumar, T. Kumar, A. Panneerselvam, N. Nadimuthu and R. Saravanamuthu. 2006. Studies on antagonism of some soil fungi against Drechslera ellisiia Fruit Rot Causing Organism in Guava. J. Soil Biol. 26: 117-122.

Panneerselvam, A. and R. Saravanamuthu. 1994. Studies on antagonistic interaction of some soil fungi against Fusarium moniliforme. J. Ind. Bot. Soc. 73: 265-67.

Porter, C.L. 1924. Concerning the characters of certain fungi as exhibited by their growth in the presence of other fungi, AM. J. Bot. 11: 168-188.

PloetzR.C, J.L Haynes and A. Vazquez. 1999. Responses of new banana accessions in South Florida to Panama disease. Crop Prot. 18: 445–449

Rakh, R.R., L.S. Raut, S.M. Dalvi and A.V. Manwar. 2011. Biological control of Sclerotium rolfsii, causing stem rot of groundnut by Pseudomonas cf. monteilii 9. Rec. Res. Sci. Technol. 3:3.

Roberts, A., H. Nazli, M. Wach and Y. Zafar. 2012. An analysis of the development and regulation of agricultural biotechnology in Pakistan.

Rojo, N., G. Gallastegi, A. Barona, L. Gurtubay, G. Ibarra-Berastegi and A. Elías. 2010. Biotechnology as an alternative for carbon disulfide treatment in air pollution control. Environ. Rev. 18: 321-332.

Rutherford, M. and A. Viljoen. 2003. Fusarium wilt of banana in Africa current status, recent research and management prospects. Presentation at: International Fusarium Wilt Symposium, Salvador, Brazil. CABI International, Egham, UK.

Saremi, F., G. Lange and B. Tieke. 1996. Deeply colored self‐assembled multilayers of anionic DPP bolaamphiphiles and cationic polyelectrolytes. Adv. Mater. 8(11): 923-926.

Sherwood, R.T. and D.J. Hagedorn. 1958. Determining common root rot potential of pea fields.

Sivan, A. and I. Chet. 1993. Integrated control of Fusarium crown and root rot of tomato with Trichoderma harzianum in combination with methyl bromide or soil solarization. Crop Prot. 12(5): 380-386.

Skidmore, A.M. and C.M. Dickson. 1976. Colony interactions and hyphae interferences between Septorianodorum and phylloplane fungi. Trans. Br. Mycol. Soc. 66: 57-64.

Smith, A.G., J.K. Heath, D.D. Donaldson, G.G. Wong, J. Moreau, M. Stahl and D. Rogers. 1988. Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. Nature. 336(6200): 688-690.

Srivastava, R., A. Khalid, U.S. Singh and A.K. Sharma. 2010. Evaluation of arbuscular mycorrhizal fungus, fluorescent Pseudomonas and Trichoderma harzianum formulation against Fusarium oxysporum f. sp. lycopersici for the management of tomato wilt. Biol. Cont. 53(1): 24-31.

Steel, R.D.D., J.H. Torrie and D. Dicky. 1997. Principles and Procedures of Statistics: A Biometrical Approach. 3rdEdn. Mc. Graw Hill Book Co. Inc. New York, USA.

Thangavelu, R. and M.M. Mustaffa. 2010. First report on the occurrence of a virulent strain of Fusarium wilt pathogen (Race-1) infecting Cavendish (AAA) group of bananas in India. Plant Disease, 94(11): 1379-1379.

Ullah, M.H., M.A. Khan, S.T. Sahi and A. Habib. 2011. Evaluation of antagonistic fungi against charcoal rot of sunflower caused by Macrophomina phaseolina (Tassi) Goid. African J. Environ. Sci. Technol. 5(8): 616-621.

Woo, S.L., F. Scala, M. Ruocco and M. Lorito. 2006. The molecular biology of the interactions between Trichoderma spp. Phytopathogenic fungi and plants. Phytopathol. 96: 181-185.

Yedidia, I., N. Benhamou, Y. Kapulnik and I. Chet. 2000. Induction and accumulation of PR proteins activity during early stages of root colonization by the mycoparasite Trichoderma harzianum strain T-203. Plant Physiol. Biochem. 38: 863-873.




DOI: https://doi.org/10.33866/phytopathol.029.01.0299

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