Naureen Akhtar, Rahila Hafeez, Waheed Anwar


Combretum indicum leaf spot disease was observed during a survey of horticultural plants in Lahore. Isolation of causal organism was carried out from the disease leaves. The pathogen, Alternaria brassicae, was first identified considering morphological characters. The morphology based identification was confirmed by nucleotide sequence analysis of ITS region of rDNA and phylogentic analysis of this fungus with closely related other fungal species. Pathogenicity of A. brassicae was verified following the Koch’s pathogenicity postulates. This report represents the first record of leaf spot of Combretum indicum by Alternaria brassicae from Pakistan.


Alternaria brassicae, Combretum indicum, leaf spot, pathogenicity

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