Muhammad Aslam, Javed A. Shah, Niaz Hussain, Abdul Ghaffar, Muneer Abbas, Muhammad F. Hussan, Aftab A. Khan, Muhammad Nadeem, Muhammad Irshad


Chickpea (Cicer arietinum L.) is a major food legume which ranks third in the world. Wilt and blight, two damaging diseases of chickpea, are prevalent in Pakistan, and this has resulted in a low output of the crop. Except for exploiting the host plant's resistance mechanism, the available control techniques are neither practicable nor cost-effective. To determine the sources of resistance in chickpea germplasm that is currently available.278chickpea genotypes (168 Desi and 110 Kabuli) originated from different springs were evaluated for disease resistance against Ascochyta rabiei in poly house and 102 different genotypes were screened for Fusarium oxysporum resistant sources in the sick field at Arid Zone Research Institute Bhakkar. Experiments were laid out following augmented design without replications during 2019-20 and highly susceptible check (AUG-424) was replicated as indicator for disease advent. The disease frequency was assessed twice at different growth stages and genotypes were categorized as per ICARDA rating scale (1-9). Ascochyta blight incidences revealed that39 lines displayed resistant (R) reaction and 12 had moderately resistant reaction (MR) in desi. However, 38 were found resistant and 26 genotypes were recorded as moderately resistant in kabuli chickpea, respectively. Similarly, 102 genotypes were sown in sick plot for screening against F. oxysporum, out of which only10 entries showed resistant and 21 moderately resistant reaction. The collected information was the most valuable to be used in breeding program for exploiting the genetic resistance and its direct use in severely blight and wilt hit areas may be preferred on the basis of resistance type.


Chickpea, genotypes, susceptibility, resistance, Ascochyta rabiei, Fusarium oxysporum, Pakistan

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Agrios, G. 2005. Plant pathology fifth edition. Ed Elsevier Academia Press. San Diego Calf. USA.

Ahmad, Z., A. S. Mumtaz, M. Nisar and N. Khan. 2012. Diversity analysis of chickpea (Cicer arietinum L.) germplasm and its implications for conservation and crop breeding. Agricultural Sciences, 03: 723-731.

Alam, S. S., R. N. Strange and S. H. Qureshi. 1987. Isolation of Ascochyta rabiei and a convenient method for copious inoculum production. Mycologist, 1: 20.

Andam, A., A. Azizi, M. Majdi and J. Abdolahzadeh. 2020. Comparative expression profile of some putative resistance genes of chickpea genotypes in response to ascomycete fungus, Ascochyta rabiei (Pass.) Labr. Brazilian Journal of Botany, 43: 123-130.

Asnakech, T. 2014. Genetic analysis and characterization of faba bean (Vicia faba) for resistance to chocolate spot (Botrytis fabae) disease and yield in the Ethiopian high lands. PhD dissertation, University of KwaZulu-Natal, Pietermaritzburg. Rep South Africa p: 266.PhD dissertation, University of KwaZulu-Natal, Pietermaritzburg. Rep South Africa: 266.

Asrat, Z and B. Tolesa. 2020. Screening of chickpea genotypes for resistance to Fusarium wilt (Fusarium oxysporum f. sp. ciceris) under field conditions in Ethiopia. African Journal of Agricultural Research, 15: 85-89.

Asrat, Z. and B. Tolesa. 2018. Evaluation of improved chickpea varieties for resistance to Fusarium wilt (Fusarium oxysporum) under field condition in sick plot. African Journal of Agricultural Research, 13: 2930-2935.

Atta, B. M., M. Haq, T.M. Shah, S. Alam, H. Ali, and K. Akhtar. 2006. Chickpea Germplasm Screening for Resistance against Ascochyta Blight. Caderno de Pesquisasérie Biologia, 18: 137-150.

Ayana, A., N. Hailu and W. Taye. 2019. Screening Desi and Kabuli Chick Pea Varieties against Fusarium Wilt Resistance in West Gojam, Northwestern Ethiopia. Journal of Plant Pathology & Microbiology, 10: 2.

Bakhsh, A., S. M. Iqbal and I. Haque. 2007. Evaluation of chickpea germplasm for wilt resistance. Pakistan Journal of Botany, 39: 583-593.

Bashir, A., M. Yaqoob, M. Rahim and R. Khalid. Najibullah 2006. Source of resistance against disease complex in chickpea. Indus Journal of Biological Sciences, 3: 660-663.

Bekele, S., R. Jones, S. Silim, H. Teklewold and E. Gwata. 2007. Analysis of production costs, market opportunities and competitiveness of Desi and Kabuli chickpeas in Ethiopia. IPMS Working Paper 3.Nairobi (Kenya): ILRI. PP. 48.

Bhardwaj, R., J. S. Sandhu, L. Kaur, S. K. Gupta, P. M. Gaur and R. Varshney. 2010. Genetics of Ascochyta blight resistance in chickpea. Euphytica, 171: 337-343.

Bokhari, A. A., M. Ashraf, A. Rehman, A. Ahmad and M. Iqbal. 2011. Screening of chickpea germplasm against Ascochyta blight. Pakistan Journal of Phytopathology, 23: 05-08.

Chaudhry, M. A., F. Muhammad and M. Afzal. 2006. Screening of chickpea germplasm against Fusarium wilt. Journal of Agricultural Research, 44: 307-312.

Chaudhry, M. A., M. Faquir and A. Muhammad. 2005. Screening of chickpea germplasm for resistance to Ascochyta blight. Journal of Agricultural Research, 43: 229-223.

Collard, B. C. Y., E. C. K. Pang, P. K. Ades and P. W. J. Taylor. 2003. Preliminary investigation of QTLs associated with seedling resistance to Ascochyta blight from Cicer echinospermum, a wild relative of chickpea. Theoretical and Applied Genetics, 107: 719-729.

Duzdemir, O., B. Selvi, Y. Yanar and A. Yildirimi. 2014. Sources of resistance in chickpea (Cicer arietinum L.) land races against Ascochyta rabiei causal agent of ascochyta blight disease. Pakistan Journal of Botany, 46: 1479-1483.

Gan, Y. T., K. H. M. Siddique, W. J. MacLeod and P. Jayakumar. 2006. Management options for minimizing the damage by ascochyta blight (Ascochyta rabiei) in chickpea (Cicer arietinum L.). Field Crops Research, 97: 121-134.

GOP. 2020. Economic Survey of Pakistan Economic Advisor’s Wing, Finance Division, Government of Pakistan, Islamabad.

Hassan, M., M. Mohsan, G. Mohy-ud-Din and A. Zia. 2012. Evaluation of chickpea germplasm lines against Ascochyta blight. Pakistan Journal of Phytopathology, 24: 117-121.

Haware, M. and Y. Nene. 1980. Sources of resistance to wilt and root rots of chickpea. International Chickpea Newsletter, 3: 11-12.

Hirich, A., H. El Omari, S. E. Jacobsen, N. Lamaddalena, A. Hamdy, R. Ragab, A. Jelloul and R. Choukr-Allah. 2014. Chickpea ('Cicer arietinum L.) physiological, chemical and growth responses to irrigation with saline water. Australian Journal of Crop Science, 8: 646-654.

Ilyas, M., M. Chaudhry, N. Javed, M. Ghazanfar and M. A. Khan. 2007. Sources of resistance in chickpea germplasm against Ascochyta blight. Pakistan Journal of Botany, 39: 1843-1847.

Iqbal, S. M. 2005. Screening of chickpea genotypes for resistance against Fusarium wilt. Mycopath, 3: 1-5.

Iqbal, S. M., A. Bakhsh, M. A. Zahid and A. M. Haqqani. 2004. Screening for resistance against Ascochyta blight in chickpea. Mycopath, 2: 7-10.

Iqbal, S. M., A. Ghafoor, A. Bakhsh, I. Ahmad and A. Sher. 2010. Identification of resistant sources for multiple disease resistance in chickpea. Pakistan Journal of Phytopathology, 22: 89-94.

Islam, M. 2011. Growth, nitrogen fixation and nutrient uptake by chickpea (Cicer arietinum) in response to phosphorus and sulfur application under rainfed conditions in Pakistan. International Journal of Agriculture and Biology, 13.

Islam, W., M. Qasim, A. Noman, A. Idrees and L. Wang. 2017. Genetic resistance in chickpea against Ascochyta blight: Historical efforts and recent accomplishments. The Journal of Animal & Plant Sciences, 27: 1941-1957.

Jamil, F. F., M. Sarwar, N. Sarwar, J. A. Khan, M. H. Zahid, S. Yousaf, H. M. I. Arshad and I. Haq. 2010. Genotyping with RAPD markers resolves pathotype diversity in the Ascochyta blight and Fusarium wilt pathogens of chickpea in Pakistan. Pakistan Journal of Botany, 42: 1369-1378.

Jan, H. and M. Wiese. 1991. Virulence forms of Ascochyta rabiei affecting chickpea in the Palouse. Plant Disease, 75: 904-906.

Jiménez-díazR.M., a. M. M. J.-g. 2011. Integrated management of Fusarium Wilt diseases. Transworld Research Network: 177-215.

Karimi, K., J. Amini, B. Harighi and B. Bahramnejad. 2012. Evaluation of biocontrol potential of 'pseudomonas' and 'bacillus' spp. against fusarium wilt of chickpea. Australian Journal of Crop Science, 6: 695-703.

Khan, I. A., S. Alam and A. Jabbar. 2004. Purification of phytotoxin from culture filtrates of Fusarium oxysporum f. sp. ciceris and its biological effects on chickpea. Pakistan Journal of Botany, 36: 871-880.

Khilare, V., R. Ahmed, S. Chavan and O. Kohire. 2009. Management of Fusarium oxysporum f. sp. ciceri by different fungicides. BIOINFOLET-A Quarterly Journal of Life Sciences, 6: 41-43.

Landa, B. B., J. A. Navas-Cortés and R. M. Jiménez-Díaz. 2004. Integrated Management of Fusarium Wilt of Chickpea with Sowing Date, Host Resistance, and Biological Control. Phytopathology®, 94: 946-960.

Maitlo, S., R. Syed, M. Rustamani, R. Khuhro and A. Lodhi. 2014. Comparative efficacy of different fungicides against fusarium wilt of chickpea (Cicer arietinum L.). Pakistan Journal of Botany, 46: 2305-2312.

Malik, S. R., M. Saleem, U. Iqbal, M. A. Zahid, A. Bakhsh and S. Iqbal. 2011. Genetic Analysis of Physiochemical Traits in Chickpea (Cicer arietinum) Seeds. International Journal of Agriculture and Biology, 13: 1033-1036.

Maqbool, M. A., M. Aslam and H. Ali. 2017. Breeding for improved drought tolerance in Chickpea (Cicer arietinumL.). Plant Breeding, 136: 300-318.

Merkuz, A. and A. Getachew. 2012. Influence of chickpea Fusarium wilt (Fusarium oxysporum f. sp. ciceris) on Desi and Kabuli-type of chickpea in integrated disease management option at wilt sick plot in North Western Ethiopia. International Journal of Current Research, 4: 45-46.

Millan, T., H. J. Clarke, K. H. M. Siddique, H. K. Buhariwalla, P. M. Gaur, J. Kumar, J. Gil, G. Kahl and P. Winter. 2006. Chickpea molecular breeding: new tools and concepts. Euphytica, 147: 81-103.

MoARD. 2008. Ministry of agriculture and rural development crop variety development. Depart Crop Variety 11.

Muehlbauer, F. J., W. J. Kaiser and I. Kusmenoglu. 1998. Registration of ‘Sanford’chickpea. Crop Science, 38: 282-283.

Naqvi, S. K. B., S. Ahmed, C. A. Rauf and S. S. Naqvi. 2013. Amplification and sequencing of internal transcribed regions 1 & 2, and 5.8 S rDNA from local isolates of fusarium species. Pakistan Journal of Botany, 45: 301-307.

Navas-Cortés, J. A., B. Hau and R. M. Jiménez-Díaz. 2000. Yield Loss in Chickpeas in Relation to Development of Fusarium Wilt Epidemics. Phytopathology, 90: 1269-1278.

Nene, Y. 1980. Diseases of chickpea. Proceedings of the International Workshop on Chickpea Improvement, Hyderabad, 28 Feb-2 Mar 1979. ICRISAT, 171-178.

Pande, S., K. H. M. Siddique, G. K. Kishore, B. Bayaa, P. M. Gaur, C. L. L. Gowda, T. W. Bretag and J. H. Crouch. 2005. Ascochyta blight of chickpea (Cicer arietinum L.): a review of biology, pathogenicity, and disease management. Australian Journal of Agricultural Research, 56: 317.

Pande, S., M. Sharma, P. M. Gaur, S. Tripathi, L. Kaur, A. Basandrai, T. Khan, C. L. L. Gowda and K. H. M. Siddique. 2011. Development of screening techniques and identification of new sources of resistance to Ascochyta blight disease of chickpea. Australasian Plant Pathology, 40: 149-156.

Randhawa, M., S. Sahi, M. Ilyas, M. Ghazanfar and N. Javed. 2009. Comparative assessment of density of glandular hairs, population and size of aperture of stomata in resistant and susceptible cultivars of chickpea to Ascochyta blight disease. Pakistan Journal Botany, 41: 121-129.

Reddy, M. and K. Singh. 1993. Rate-reducing resistance to Ascochyta blight in chickpeas. Plant disease, 77: 231-233.

Sarwar, N., K. P. Akhtar, T. M. Shah and B. M. Atta. 2012. Evaluation of chickpea advance genotypes against blight and wilt diseases under field conditions. International Journal of Agriculture and Biology, 14: 993-996.

Seid, A. and M. Ayalew. 2006. Chickpea, lentil, grass pea, fenugreek and lupine disease research in Ethiopia. Food and forage legumes of Ethiopia progress and prospects. ICARDA, Addis Ababa, Ethiopia: 215-220.

Shah, T. M., B. M. Atta, J. I. Mirza and M. A. Haq. 2009. Screening of chickpea (Cicer arietinum) induced mutants against Fusarium wilt. Pakistan Journal of Botany, 41: 1945-1955.

Shah, T. M., M. Haq, B. Atta, S. Alam and H. Ali. 2005. Evaluation of Cicer species for resistance to Ascochyta blight. Pakistan Journal of Botany, 37: 431.

Shahbaz, M., M. Azhar Iqbal, A. Mustafa, M. Intizar ul-Hassan and F. A. Faiz. 2014. Host plant resistance of different chickpea genotypes against Ascochyta rabiei (Pass.) Lab. under tunnel conditions. Archives of Phytopathology and Plant Protection, 47: 529-536.

Sharma, K. D. and F. J. Muehlbauer. 2007. Fusarium wilt of chickpea: physiological specialization, genetics of resistance and resistance gene tagging. Euphytica, 157: 1-14.

Sharma, K. D., W. Chen and F. J. Muehlbauer. 2005. Genetics of Chickpea Resistance to Five Races of Fusarium Wilt and a Concise Set of Race Differentials for Fusarium oxysporum f. sp. ciceris. Plant Disease, 89: 385-390.

Sharma, M. and R. Ghosh. 2016. An Update on Genetic Resistance of Chickpea to Ascochyta Blight. Agronomy, 6: 18.

Shivalinga, H. 2018. Screening for resistant sources in chickpea entries against Fusarium wilt. Journal of Pharmacognosy and Phytochemistry, 7: 663-665.

Tariq M.S, M. Imran, B. M. Atta, M. Shafiq, M. Aslam and K. Hussain. 2015. Screening of chickpea advanced lines for sources of resistance against blight and wilt two major diseases of chickpea. Pakistan Journal of Botany, 47: 2443-2448.

Tekeoglu, M., D. K. Santra, W. J. Kaiser and F. J. Muehlbauer. 2000. Ascochyta Blight Resistance Inheritance in Three Chickpea Recombinant Inbred Line Populations. Crop Science, 40: 1251-1256.

Thaware, D., V. Gholve and P. Ghante. 2016. Screening of Chickpea Varieties, Cultivars and Genotypes against Fusarium oxysporum f. sp. ciceri. International Journal of Current Microbiology and Applied Sciences, 6: 896-904.

Toker, C., B. Uzun and M.I. Cagirgan. 1999. Screening and selection for resistance to Ascochyta blight [Ascochyta rabiei(Pass) Labr.] of chickpea (Cicer arietinum L.) under field conditions. Turkish Phytopathology, 28: 101-110.

Varshney, R. J., M. Thudi, S.N. Nayak, P.M. Gaur, J. Kashiwagi, L. Karishnamurhy,D. Jaganathan, K. Koppolu, A. Bohra, S. Tripathi, A. Rathore, A.K. Jukanti, V. Jayalakshmi, A. Vimula, S.J. Singh, M. Yasin, M.S. SheshshayeeandK.P. Viswanatha. 2014. Genetic dissection of drought tolerance in chickpea (Cicer arietinum L.). Theoratical and Applied Genetics, 127: 445-462.



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