Isozymes and bio-control analysis of Fusarium spp. from post harvest diseased mango

Sadia Fida Ullah (sadia.fida@ymail.com)
Environmental sciences, Fatima Jinnha Women University Rawalpindi
August, 2014
 

Abstract
Electrophoretic studies of Isozymes and whole cell protein using SDS-PAGE was carried out to evaluate the genetic diversity and intra specific variations among Fusarium solani and oxysporum causative fungal pathogens of mango rotting in Pakistan. In present study 47 isolates studied for morphological variability and invitro bio-efficiency of plants against Fusarium spp. Among them 37 isolates randomly selected for molecular analysis. Results revealed morphological characteristics of isolates were similar to each other. By using four isozymes (Lactate dehydrogenase, Malate dehydrogenase, Glucose-6-phosphate dehydrogenase, β-glucosidase) 24 phenotypes were obtained. Among studied isolates MDH exhibit lowest activity. Isozymes banding pattern obtained by SDS-PAGE used to compute Jaccard’s, similarity coefficients ranged from 0.25 to 1.0 indicating genetic diversity in isolates. Cluster analysis with UPGMA showed that isolates belonged to two main groups. Genetic distance observed D=0.15 to D=1.0. Among 37 isolates 90% belongs to Fusarium solani. Three plant species Azadirachta indica, Moras alba and Citrus lemon.L used as anti-Fungal Botanicals (AFB) to control the growth of Fusarium spp. Comparative analysis of all three AFBs showed Moras alba most effective against the Fusarium solani and oxysporum. Anova showed that all genotypes and treatment was significantly different from each other at 5% probability level. In conclusion, cluster analysis of the protein banding patterns by SDS-PAGE, and electrophoretic detection of isozymes banding patterns were useful tools for differentiating Genetic diversity among Fusarium spp. and AFBs preferred to commonly used fungicides because of their environment friendly nature. This understanding can aid in devising ways of managing this potentially pathogenic fungus.