INCIDENCE OF PANTOEA AGGLOMERANS ASSOCIATED WITH BARNYARD GRASS (ECHINOCHLOA CRUSGALLI L.) IN PAKISTAN

Sehrish Mushtaq, Muhammad Shafiq, Ummad Hussain, Freeha Ali, Amna Ali, Faiza Khan, Muhammad Asim, Saleem S. Haider

Abstract


Weeds are reservoirs for the survival of pathogenic microorganisms and cause of spread of infectious disease of plants through vectors. To minimize the spread of bacteria to other host crops, proper characterization is required to suggest suitable control strategy. Survey was conduct in December 2015, leaf blight was observed on barnyard grass growing in surroundings of citrus orchards located in Lahore, Pakistan. Isolation of the bacterium on nutrient agar medium was performed and their characterization based on morphological and biochemical tests. 16S rRNA was amplified directly with primers 27F/1492R, cloned and sequenced. Sequences were blast in NCBI data base which showed 99% identity to P. agglomerans strain. A neighbor-joining tree was generated with reported bacterial strain that were most closely to isolated strain P. agglomerans (LT592133). Pathogenicity test was conducted on barnyard grass with bacterial suspensions having 1.5 × 108 CFU/ml in controlled conditions to confirm Koch’s postulates. According to results this is first report on the incidence of Pantoea agglomerans on Barnyard grass in Pakistan.


Keywords


Pantoea agglomerans, pathogenicity test, barnyard grass, 16S rRNA analysis

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References


Andersson, A. M., N. Weiss, F. Rainey and M. S. Salkinoja‐Salonen. 1999. Dust‐borne bacteria in animal sheds, schools and children's day care centres. Journal of Applied Microbiology, 86: 622-634.

Dutkiewicz, J., B. Mackiewicz, M. Kinga Lemieszek, M. Golec and J. Milanowski. 2016. Pantoea agglomerans: a mysterious bacterium of evil and good. Part III. Deleterious effects: infections of humans, animals and plants. Annals of Agricultural and Environmental Medicine, 23: 197-205.

Feng, Y., D. Shen and W. Song. 2006. Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and affects allocations of host photosynthates. Journal of Applied Microbiology, 100: 938-945.

Monier, J. M. and S. E. Lindow. 2005. Aggregates of Resident Bacteria Facilitate Survival of Immigrant Bacteria on Leaf Surfaces. Microbial Ecology, 49: 343-352.

Morales-Valenzuela, G., H. Silva-Rojas and D. Ochoa-Martínez. 2017. First report of Pantoea agglomerans causing leaf blight and vascular wilt in maize and sorghum in Mexico. Journal of Plant Diseases and Protection, 47.

Pheng, S. 2001. Response of two rice cultivars to the competition of Echinochloa crus-galli. International Rice Research Notes: 36-37.

Ren, Y. Z., Y. Q. Liu, S. L. Ding, G. Y. Li and H. Zhang. 2008. First Report of Boll Rot of Cotton Caused by Pantoea agglomerans in China. Plant Disease, 92: 1364-1364.

Tho, K. E., P. Wiriyajitsomboon and M. K. Hausbeck. 2015. First Report of Pantoea agglomerans Causing Onion Leaf Blight and Bulb Rot in Michigan. Plant Disease, 99: 1034-1034.

Völksch, B., S. Thon, I. D. Jacobsen and M. Gube. 2009. Polyphasic study of plant- and clinic-associated Pantoea agglomerans strains reveals indistinguishable virulence potential. Infection, Genetics and Evolution, 9: 1381-1391.

Wilson, K. 2001. Preparation of genomic DNA from bacteria. Current protocols in molecular biology, 56: 2.4. 1-2.4. 5.




DOI: https://doi.org/10.33866/phytopathol.031.01.0411

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Pakistan Journal of Phytopathological
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.