Nosheen Kabir, Shahid I. Awan, Hafiz M.S.U. Rahman, Muhammad Ilyas, Adnan Idris, Muhammad Rizwan


Wheat stripe rust disease, produced by Puccinia striiformis f. sp. tritici (Pst), causes severe yield reduction worldwide. With the advent of modern wheat varieties, the sources of rust resistance are eroding. Hence a constant search for resistant genotypes is necessary. Thirty broad-based elite lines and landraces of wheat were characterized for their agronomic traits, assessed for the adult plant resistance against wheat stripe rust disease inside a trap nursery. Furthermore, the chemical response was assessed at cellular level. The landraces and elite lines displayed a diverse nature of host-pathogen interactions. The landrace LLR8 showed a hypersensitive response in the field. Seven genotypes were highly resistant while 07 were moderately resistant at the adult plant stage. The genotype RS1 showed maximum necrosis (2896 µm) indicating moderately resistant (10MR) under field conditions. Among resistant genotypes the number of hyphae at the infection site were less compared to the susceptible genotypes. The stripe rust fungal colonies were initially larger but with time the fungal colony size decreased, might be the result of the synchronized initiation of defense mechanism. The resistant genotypes also showed higher values for the hypersensitivity index. Multivariate discriminant analysis for agronomic traits divided the genotypes into low and high yielding groups, where nine genotypes were high yielding while twenty-one were low yielding under high yellow rust disease pressure. The genotypes like LLR8, Pirsabak-04 and RS4 displayed higher grain yield per plant and 1000-grain weight. The plant height and biological yield were good discriminators as they helped to discriminate between the 02 groups. The resistance sources such as LLR35, RS13, RS22, RS30, RS55, RS58, RS64, LLR5, LLR17, LLR33, RS1, RS10, RS43, and RS45 could be beneficial for the development of future cultivars with effective resistance. This genetic material should be utilized immediately for the disease management.


Histology, landraces, Triticum aestivum, Puccinia striiformis, resistance.

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Aboukhaddour, R., T. Fetch, B. D. McCallum, M. W. Harding, B. L. Beres and R. J. Graf. 2020. Wheat diseases on the prairies: A Canadian story. Plant Pathology, 69: 418-432.

Agrios, G. 2005. Plant pathology. (5th eds.) Elsevier academic Press. New York.

Ahmad, S., M. Afzal, I. Noorka, Z. Iqbal, N. Akhtar, Y. Iftkhar and M. Kamran. 2010. Prediction of yield losses in wheat (Triticum aestivum L.) caused by yellow rust in relation to epidemiological factors in Faisalabad. Pakistan Journal of Botany, 42: 401-407.

Akcura, M., A. Kadir and O. Hocaoglu. 2017. Biplot analysis of leaf rust resistance in pure lines selected from eastern Anatolian bread wheat landraces of turkey. Turkish Journal of Field Crops, 22: 227-234.

Amil, R., S. Ali, B. Bahri, M. Leconte, C. Vallavieille‐Pope and K. Nazari. 2020. Pathotype diversification in the invasive PstS2 clonal lineage of Puccinia striiformis f. sp. tritici causing yellow rust on durum and bread wheat in Lebanon and Syria in 2010–2011. Plant Pathology, 69: 618-630.

Awan, S. I., S. D. Ahmad, L. Mur and M. S. Ahmed. 2017. Marker-Assisted Selection for Durable Rust Resistance in a Widely Adopted Wheat Cultivar" Inqilab-91". International Journal of Agriculture and Biology, 19: 1319-1324.

Azim, R., M. Ilyas, S.I. Awan, H. Tariq and N. Iqbal. 2018. Assessment of morphological and biochemical diversity of Berberis lycium in three districts of Azad Jammu and Kashmir. International Journal of Biosciences., 12: 37-47.

Bolton, M. D., J. A. Kolmer and D. F. Garvin. 2008. Wheat leaf rust caused by Puccinia triticina. Molecular Plant Pathology, 9: 563-575.

Carmona, M., F. Sautua, O. Pérez-Hérnandez and E. M. Reis. 2020. Role of Fungicide Applications on the Integrated Management of Wheat Stripe Rust. Frontiers in Plant Science, 11: 733-733.

Carver, T. L. W., M. P. Robbins and R. J. Zeyen. 1991. Effects of two PAL inhibitors on the susceptibility and localized auto fluorescent host cell responses of oat leaves attacked by Erysiphe graminis DC. Physiological and Molecular Plant Pathology, 39: 269-287.

Chen, H., M. Iqbal, R. C. Yang, and D. Spaner. 2016. Effect of Lr34/Yr18 on agronomic and quality traits in a spring wheat mapping population and implications for breeding. Molecular Breeding, 36: 53.

Cheng, P., L. S. Xu, M. N. Wang, D. R. See and X. M. Chen. 2014. Molecular mapping of genes Yr64 and Yr65 for stripe rust resistance in hexaploid derivatives of durum wheat accessions PI 331260 and PI 480016. Theoretical and Applied Genetics, 127: 2267-2277.

Elahinia, S. 2008. Microscopic study on expression of Yr-18 gene related to adult plant resistance in a near-isogenic line of spring wheat (Triticum aestivum L.) to the Stripe Rust (Puccinia striiformis f. sp. tritici). Journal of Agriculture Science and Technology, 10: 359-369.

Fedotova, L. and B. Bankina. 2018. Characterization of yellow rust (Puccinia striiformis Westend.). Research for Rural Development, 2: 69-76.

Fisher, R. A. 1936. The use of multiple measurements in taxonomic problems. Annals of Eugenics, 7: 179-188.

Gao, X., B.-T. Wang and F. Wang. 2000. Identification of slow-rusting entries in resistance breeding programs to wheat stripe rust and establishment of quantified criteria for their distinction. Acta Agronomica Sinica, 26: 372-376.

Gessese, M. K. 2019. Description of Wheat Rusts and Their Virulence Variations Determined through Annual Pathotype Surveys and Controlled Multi-Pathotype Tests. Advances in Agriculture, 2019: 1-7.

Gul, B., S. Ijaz and H. Khan. 2019. Allelopathic Effect of Lotus and Arrowhead Weed on Wheat, Wild Oat and Milkthistle Germination. Planta Daninha, 37: e019184290.

Hafeez, S., L. Zhangyong, J. Tao, S.I. Awan, I. Ahmad, T. Anwar, R. Sharif, N.M. Sylvain and C. Chambi. 2016. Assessment of drought tolerance in spring wheat (Triticum aestivum L.) at different growth stages under the rainfed conditions of Rawalakot Azad Kashmir Pakistan. Global Advanced Research Journal of Agricultural Science, 5: 132-140.

Hair, J. F., R.E. Anderson and R.L. Tatham. Prentice Hall, 2009. Black WC. Multivariate Data Analysis. New York, NY, USA.

Hammer, Ø., D. A. Harper and P. D. Ryan. 2001. PAST: Paleontological statistics software package for education and data analysis. Palaeontologia electronica, 4: 9.

Hovmoller, M., S. Walter, R. Bayles, A. Hubbard, K. Flath, N. Sommerfeldt, M. Leconte, P. Czembor, J. Rodriguez‐Algaba and T. Thach. 2016. Replacement of the European wheat yellow rust population by new races from the centre of diversity in the near‐Himalayan region. Plant Pathology, 65: 402-411.

Imran, J., S. Awan, H. Ahmad and A. Rao. 2016. Assesment of Genetic Diversity in Wheat Synthetic Double Haploids for Yield and Drought Related Traits Through Factor and Cluster Analyses. Plant Gene and Trait, 7: 1-9.

Jin, Y., L. J. Szabo and M. Carson. 2010. Century-Old Mystery of Puccinia striiformis Life History Solved with the Identification of Berberis as an Alternate Host. Phytopathology, 100: 432-435.

Kankwatsa, P., D. Singh, P. C. Thomson, E. M. Babiker, J. M. Bonman, M. Newcomb and R. F. Park. 2017. Characterization and genome-wide association mapping of resistance to leaf rust, stem rust and stripe rust in a geographically diverse collection of spring wheat landraces. Molecular Breeding, 37: 1-24.

Kutlu, I. and Z. Sirel. 2019. Using line × tester method and heterotic grouping to select high yielding genotypes of bread wheat (Triticum aestivum L.). Turkish Journal of Field Crops: 185-194.

Lebart, L., A. Mirineau and M. Piron. 2006. Statistique Exploratoire Multidimensionelle. ed. Dound, Paris, France, 4.

Leogering, W. 1959. Methods for recording cereal rust data USDA international spring wheat rust nursery.

Long, L., F. Yao, C. Yu, X. Ye, Y. Cheng, Y. Wang, Y. Wu, J. Li, J. Wang, Q. Jiang, W. Li, J. Ma, Y. Liu, M. Deng, Y. Wei, Y. Zheng and G. Chen. 2019. Genome-Wide Association Study for Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landraces (Triticum aestivum L.) From the Yellow and Huai River Valleys. Frontiers in Plant Science, 10: 596-596.

Ma, J., E. Ma, H. Xu, K. Yagi, and Z. Cai. 2009. Wheat straw management affects CH4 and N2O emissions from rice fields. Soil Biology and Biochemistry, 41: 1022-1028.

Minker, K. R., M. L. Biedrzycki, A. Kolagunda, S. Rhein, F. J. Perina, S. S. Jacobs, M. Moore, T. M. Jamann, Q. Yang, R. Nelson, P. Balint-Kurti, C. Kambhamettu, R. J. Wisser and J. L. Caplan. 2018. Semiautomated confocal imaging of fungal pathogenesis on plants: microscopic analysis of macroscopic specimens. Microscopy Research and Technique, 81: 141-152.

Niks, R. E., X. Qi and T. C. Marcel. 2015. Quantitative Resistance to Biotrophic Filamentous Plant Pathogens: Concepts, Misconceptions, and Mechanisms. Annual Review of Phytopathology, 53: 445-470.

Peterson, R. F., A. B. Campbell and A. E. Hannah. 1948. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian Journal of Research, 26c: 496-500.

Rehman, A. u., S. A. H. Naqvi, U.-u.-D. Umar, M. I. Zafar, F. Hussain, M. A. Zulfiqar and A. A. Khan. 2019. Identification of Resistance Sources in Wheat to Brown and Yellow Rust. Pakistan Journal of Agricultural Research, 32: 185-196.

Saleem, K., C. K. Sørensen, R. Labouriau and M. S. Hovmøller. 2019. Spatiotemporal changes in fungal growth and host responses of six yellow rust resistant near‐isogenic lines of wheat. Plant Pathology, 68: 1320-1330.

Shirdelmoghanloo, H., D. Cozzolino, I. Lohraseb and N. C. Collins. 2016. Truncation of grain filling in wheat (Triticum aestivum) triggered by brief heat stress during early grain filling: association with senescence responses and reductions in stem reserves. Functional Plant Biology, 43: 919-930.

Singh, R. P. and J. Huerta‐Espino. 1997. Effect of Leaf Rust Resistance Gene Lr34 on Grain Yield and Agronomic Traits of Spring Wheat. Crop Science, 37: 390-395.

Singh, R. P., P. K. Singh, J. Rutkoski, D. P. Hodson, X. He, L. N. Jørgensen, M. S. Hovmøller and J. Huerta-Espino. 2016. Disease Impact on Wheat Yield Potential and Prospects of Genetic Control. Annual Review of Phytopathology, 54: 303-322.

Singh, R., E. Duveiller and J. Huerta-Espino. 2012. Virulence to yellow rust resistance gene Yr27: a new threat to stable wheat production in Asia. Meeting the Challenge of Yellow Rust in Cereal Crops. 25-29.

Snedecor, G. W. and W. G. Cochran. 1989. Statistical methods, 8thEdn. Ames: Iowa State Univ. Press Iowa, 54: 71-82.

Sorensen, C. K., R. Labouriau and M. S. Hovmoller. 2017. Temporal and Spatial Variability of Fungal Structures and Host Responses in an Incompatible Rust-Wheat Interaction. Frontiers in Plant Science, 8: 484-484.

Sorrells, M. E. 1998. Marker assisted selection: is it practical. Application of biotechnology to wheat breeding: 103-110.

Spielmeyer, W., R. A. McIntosh, J. Kolmer and E. S. Lagudah. 2005. Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theoretical and Applied Genetics, 111: 731-735.

SPSS. 2011. The SPSS System for Windows. Release 20.0.0 SPSS Inc., IBM Company Headquarters, USA.

Thach, T., S. Ali, C. de Vallavieille-Pope, A. F. Justesen and M. S. Hovmøller. 2016. Worldwide population structure of the wheat rust fungus Puccinia striiformis in the past. Fungal Genetics and Biology, 87: 1-8.

Toruno, T. Y., I. Stergiopoulos and G. Coaker. 2016. Plant-Pathogen Effectors: Cellular Probes Interfering with Plant Defenses in Spatial and Temporal Manners. Annual review of phytopathology, 54: 419-441.

Walter, S., S. Ali, E. Kemen, K. Nazari, B. A. Bahri, J. Enjalbert, J. G. Hansen, J. K. M. Brown, T. Sicheritz-Pontén, J. Jones, C. de Vallavieille-Pope, M. S. Hovmøller and A. F. Justesen. 2016. Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis. Ecology and Evolution, 6: 2790-2804.

Wellings, C. R. 2011. Global status of stripe rust: a review of historical and current threats. Euphytica, 179: 129-141.

Xie, Q., S. Mayes and D. L. Sparkes. 2015. Optimizing tiller production and survival for grain yield improvement in a bread wheat × spelt mapping population. Annals of Botany, 117: 51-66.

Yuan, F.-P., Q.-D. Zeng, J.-H. Wu, Q.-L. Wang, Z.-J. Yang, B.-P. Liang, Z.-S. Kang, X.-H. Chen and D.-J. Han. 2018. QTL Mapping and Validation of Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Humai 15. Front Plant Science, 9: 968-968.

Zhang, Q., B. Wang, J. Wei, X. Wang, Q. Han and Z. Kang. 2018. TaNTF2, a contributor for wheat resistance to the stripe rust pathogen. Plant Physiology and Biochemistry, 123: 260-267.

DOI: https://doi.org/10.33866/phytopathol.033.02.0636


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