Shomaila Iqbal, Muhammad Ashfaq, Aamir H. Malik, Muhammad I.U. Haq, khalid S. khan


The study's major goal was to find more efficient antagonist fungus for soil-borne diseases of chilli and tomato plants. Trichoderma species (T.asperellum and T.viride) were investigated for antifungal potential against Pakistani and American soil-borne pathogenic isolates of Pythium aphendermatum, P. capsici, P. infestant, and F. oxysporum. In-vivo conformation aggressiveness was tested using Trichoderma strains that had been identified morphologically and molecularly. Both biocontrol agents (T.asperellum and T.viride) were found effective to suppress the pathogenic Fusarium oxysporum isolate; T.viride, on the other hand, suppressed Phytophthora spp. (40%) and T.asperellum reduced growth up to 70% of  Pythium aphendermatum isolates from the United States of America. T.viride and T.asperellum showed similar degrees of inhibition against pathogenic fungi (Pythium, Phytophthora, and Fusarium) of Pakistani isolates (60-90%). Both BCA fungi were treated simultaneously and maximum inhibition was observed up to 90%. Whereas T. asperllum was found more efficient than T. viride to inhibit the infection by Pythium, Phytophthora, and Fusarium species. When antagonistic fungi were coupled, the Trichoderma strain demonstrated broad spectrum antagonism effect, which is a critical characteristic for the economic sustainability of any biocontrol agent.


Confrontation, Dual culture, Biocontrol fungi, Antifungal poential

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