Molecular Characterization of Pathogen, Infecting Capsicum Frutescene (Chilli Papper) And Solanum Lycopersicum (Tomato) In Lahore, Pakistan
DOI:
https://doi.org/10.33866/phytopathol.036.02.1068Keywords:
Begomovirus, Fungal Pathogens, Solanaceae, Whitefly, ITS, cp Core primersAbstract
Chili pepper (Capsicum frutescens L.) and tomato (Solanum lycopersicum L.) are the herbaceous plants that are cultivated all over the world. In recent years, the production of chili peppers and tomatoes has been hampered by several diseases. This study was carried out for molecular characterization of pathogens infecting Capsicum frutescens L. and Solanum lycopersicum L. The Leaves with disease-associated symptoms like leaf spots, curling, wilting, and yellowing of tips and veins, were collected from vicinity of the Lahore city. For molecular characterization, DNA was isolated from infected leaves through CTAB method. Internal Transcribed Spacer primers (ITS1-F, ITS4-R) were used for identification of fungal pathogens and Primers targeting the viral cp-core region were applied to find viral pathogens. Rolling Circle Amplification (RCA) was completed using random primers to amplify circular viral genomes. The amplicon of Polymerase chain reaction (PCR) of 600bp was observed on 1% agarose gel electrophoresis representing geminivirus. Furthermore, geminivirus-associated betasatellites of 1.4 kb and 2.8 kb genome confirmed the attack of its genus begomovirus in chili and tomato plants. Use of chemicals, screening (symptomatic or asymptomatic), cultural methods, and biocontrol are significant practices to eliminate whiteflies (Bemisia tabaci- host of begomovirus transmission) from the field, ultimately it will reduce the infection rate, and increase the production of pathogens-free chili peppers and tomatoes.References
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