ENHANCING OYSTER MUSHROOM (PLEUROTUS SPP.) YIELD PERFORMANCE ACROSS VARIOUS SUBSTRATES THROUGH CHEMICAL AND BIOLOGICAL SUPPLEMENTS

Shazia Akram, Rizwan Khan, Owais Iqbal, Muhammad Nabeel, Sumbal Zaman, Nimra Khanzada, Muhammad A. Khanzada, Muhammad A. Rajput

Abstract


Oyster mushroom Pleurotus ostreatus is economically important, edible mushroom and known for its medicinal properties. Hence, integration of non-conventional crops in prevailing agricultural system can improve the economic status of the farmer. The aim of the study was to evaluated the suitability of various substrates with addition of chemicals and biological supplements to improve yield production. The spawn was prepared in Laboratory of Plant Pathology Agriculture Peshawar from the pure culture of P. ostreatus. In this study, diverse substrate materials were collected, chopped, and treated with a combination of chemical and biological supplements (10g/L) dissolved in water, employing the lamp spawning method. Each plastic bag was incubated with 200g of prepared spawn until entire bag become white, indicating full colonization. Subsequently, the treated substrates underwent incubation at 25°C. The appreciable yields were obtained on chemical fortified substrates as compared to non-supplemented treatments which have a significant effect on the mushroom production. Overall, the highest mean yield (388 g) was recorded on wheat straw substrate supplemented with diammonium. In addition, biological supplemented substrate was significantly enhanced the yield performance of P. ostreatus expect of chicken manure substrate. The high yield (372 g) was observed on cotton waste substrate augmented with biological supplement rice husk. It is concluded that the addition of external nutrients including chemical and biological supplements can increase the yield of oyster mushroom and will be useful tool for the farmers to add supplements in substrates.


Keywords


Oyster mushroom, substrate, biological nutrients, diammonium

Full Text:

PDF

References


Ashraf, J., M. A. Ali, W. Ahmad, C. M. Ayyub and J. Shafi. 2013. Effect of different substrate supplements on oyster mushroom (Pleurotus spp.) production. Food Science and Technology, 1(3): 44-51.

Belewu, M. A. and K. Y. Belewu. 2005. Cultivation of mushroom (Volvariella volvacea) on banana leaves. African journal of Biotechnology, 4(12). 1401-1403.

Bell, V., C. R. P. G. Silva, J. Guina and T. H. Fernandes. 2022. Mushrooms as future generation healthy foods. Frontiers in Nutrition, 9: 1050099.

Bellettini, M. B., F. A. Fiorda, H. A. Maieves, G. L. Teixeira, S. Ãvila, P. S. Hornung, A. M. Junior and R. H. Ribani. 2019. Factors affecting mushroom Pleurotus spp. Saudi Journal of Biological Sciences, 26(4): 633-646.

Bhatti, M. I., M. M. Jiskani, K. H. Wagan, M. A. Pathan and M. R. Magsi. 2007. Growth, development and yield of oyster mushroom, Pleurotus ostreatus (Jacq. Ex. Fr.) Kummer as affected by different spawn rates. Pakistan Journal of Botany, 39(7): 2685-2692.

Buendía, M. R. P., A. Pardo-Giménez and J. A. de Juan-Valero. 2016. Reuse of degraded Pleurotus ostreatus (Jacq.) P. Kumm. substrate by supplementation with wheat bran. Quantitative parameters. Mycology, 7(2): 53-63.

Chanakya, H. N., S. Malayil and C. Vijayalakshmi. 2015. Cultivation of Pleurotus spp. on a combination of anaerobically digested plant material and various agro-residues. Energy for sustainable development, 27: 84-92.

Curvetto, N. R., D. Figlas, R. Devalis and S. Delmastro. 2002. Growth and productivity of different Pleurotus ostreatus strains on sunflower seed hulls supplemented with N–NH4+ and/or Mn (II). Bioresource Technology, 84(2): 171-176.

Du Plooy, G. W. 2007. Pests, pathogens, competitors and weed fungi of cultivated oyster mushrooms (Pleurotus spp) in South Africa (Unpublished) PhD thesis, University of Pretoria.

Fokunang, E. T., M. G. Annih, L. E. Abongwa, M. E. Bih, T. M. Vanessa, D. J. Fomnboh and C. Fokunang. 2022. Medicinal mushroom of potential pharmaceutical toxic importance: Contribution in phytotherapy. IntechOpen press, London, U.K.

Hoa, H. T., C. L. Wang and C. H. Wang. 2015. The effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology, 43(4): 423-434.

Hu, J., V. Arantes and J. N. Saddler. 2011. The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistic effect? Biotechnology for biofuels, 4 (36): 1-14.

Hussain, S., H. Sher, Z. Ullah, M. S. Elshikh, D. A. Al Farraj, A. Ali and A. M. Abbasi. 2023. Traditional uses of wild edible mushrooms among the local communities of Swat, Pakistan. Foods, 12(8): 1705.

Iqbal, O., C. Li and A. M. Lodhi. 2023. Antagonistic Pseudomonas: Alternative to Chemical Fungicides for the Management of Phytopathogens. In Biofungicides: Eco-Safety and Future Trends, CRC Press, Boca Raton, 216–246.

Iqbal, S. M., C. A. Rauf and M. I. Sheikh. 2005. Yield performance of oyster mushroom on different substrates. International Journal of Agriculture and Biology, 7(6): 900-903.

Kausar, T. and R. Bajwa. 2005. Incorporation of button mushrooms in Pakistani dishes. Biological Sciences-PJSIR, 48(6): 417-421.

Khan, N. A., M. Ajmal, M. I. U. Haq, N. Javed, M. A. Ali, R. Binyamin and S. A. 2012. Impact of sawdust using various woods for effective cultivation of oyster mushroom. Pakistan Journal of Botany, 44(1): 399-402.

Kishwar, S., Z. K. Shinwari and I. Farida. 2007. Diversity of edible mushrooms in Pakistan. Pakistan Journal of Agricultural Research, 20(1/2): 88-91.

Liu, Q., H. Ma, Y. Zhang and C. Dong. 2018. Artificial cultivation of true morels: current state, issues and perspectives. Critical reviews in biotechnology, 38(2): 259-271.

Mandeel, Q. A., A. A. Al-Laith and S. A. Mohamed. 2005. Cultivation of oyster mushrooms (Pleurotus spp.) on various lignocellulosic wastes. World Journal of Microbiology and Biotechnology, 21: 601-607.

Mata, G. and A. R. Estrada. 2005. Viability in spawn stocks of the white button mushroom, Agaricus bisporus, after freezing in liquid nitrogen without a cryoprotectant. Journal of Agriculture Technology, 1(1): 153-162.

Mendoza-Mendoza, A., J. Steyaert, M. F. Nieto-Jacobo, A. Holyoake, M. Braithwaite and A. Stewart. 2015. Identification of growth stage molecular markers in Trichoderma sp. ‘atroviride type B’ and their potential application in monitoring fungal growth and development in soil. Microbiology, 161(11): 2110-2126.

Miles, P. G. and S. T. Chang. 2004. Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact. CRC press, Boca Raton.

Naraian, R., R. K. Sahu, S. Kumar, S. K. Garg, C. S. Singh and R. S. Kanaujia. 2009. Influence of different nitrogen rich supplements during cultivation of Pleurotus florida on corn cob substrate. The Environmentalist, 29: 1-7.

Obodai, M., J. Cleland-Okine and K. A. Vowotor. 2003. Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. Journal of Industrial Microbiology and Biotechnology, 30(3): 146-149.

Onyango, B. O., V. A. Palapala, P. K. Axama, S. O. Wagai and B. M. Gichimu. 2011. Suitability of selected supplemented substrates for cultivation of Kenyan native wood ear mushrooms (Auricularia auricula). 17: 1-9.

Pant, D., U. G. Reddy and A. Adholeya. 2006. Cultivation of oyster mushrooms on wheat straw and bagasse substrate amended with distillery effluent. World Journal of Microbiology and Biotechnology, 22: 267-275.

Raman, J., K. Y. Jang, Y. L. Oh, M. Oh, J. H. Im, H. Lakshmanan and V. Sabaratnam. 2021. Cultivation and nutritional value of prominent Pleurotus spp.: an overview. Mycobiology, 49(1): 1-14.

Ratuchne, A., E. A. Lonardoni, C. E. Bueno, G. F. Reis, M. I. Rezende, A. Urbano and L. A. A. Panagio. 2016. Novel Fungal Biomaterial: Development and Bioremediation of Plastic Wastes. 5: 543-53.

Rodias, E., E. Aivazidou, C. Achillas, D. Aidonis and D. Bochtis. 2020. Water-energy-nutrients synergies in the agrifood sector: A circular economy framework. Energies, 14(1): 159.

Rose, P. K., S. B. Dhull and M. K. Kidwai. 2022. Cultivation of wild mushrooms using lignocellulosic biomass-based residue as a substrate. In Wild Mushrooms. CRC Press, Boca Raton. 493-520.

Rubini, A., C. Riccioni, B. Belfiori and F. Paolocci. 2014. Impact of the competition between mating types on the cultivation of Tuber melanosporum: Romeo and Juliet and the matter of space and time. Mycorrhiza, 24: 19-27.

Salami, A. O., F. A. Bankole and O. C. Andrew. 2017. Effect of organic nitrogen supplements on the yield and nutrient content of oyster mushroom (Pleurotus florida) cultivated on corncobs. SCI Fed journal of Mycology, 1(1): 1-9.

Sánchez, C. 2010. Cultivation of Pleurotus ostreatus and other edible mushrooms. Applied microbiology and biotechnology, 85: 1321-1337.

Sharma, S., R. K. P. Yadav and C. P. Pokhrel. 2013. Growth and yield of oyster mushroom (Pleurotus ostreatus) on different substrates. Journal on New Biological Reports, 2(1): 03-08.

Sobhan, A. 2006. Effect of different supplements with different levels to paddy straw substrate on the growth and yield of oyster mushroom (Unpublished) MSc thesis, Sher-e-Bangla Agricultural University, Dhaka.

Sreekrishnan, T. R., S. Kohli and V. Rana. 2004. Enhancement of biogas production from solid substrates using different techniques––a review. Bioresource technology, 95(1): 1-10.

Tariq, R. M. S., T. Ahmad, S. Aziz, S. Sattar, Z. Ahmad and W. Ahmad. 2020. An outlook of socio-economic, climatic and marketing factors for mushroom cultivation in Pakistan. Plant Protection, 4(2): 1-6.

Tesfay, T., T. Godifey, R. Mesfin and G. Kalayu. 2020. Evaluation of waste paper for cultivation of oyster mushroom (Pleurotus ostreatus) with some added supplementary materials. AMB Express, 10(15): 1-8.

Thakur, M. P. 2020. Advances in mushroom production: Key to food, nutritional and employment security: A review. Indian Phytopathology, 73: 377-395.

Vital, A. C. P., P. A. Goto, L. N. Hanai, S. M. Gomes-da-Costa, B. A. de Abreu Filho, C. V. Nakamura and P. T. Matumoto-Pintro. 2015. Microbiological, functional and rheological properties of low fat yogurt supplemented with Pleurotus ostreatus aqueous extract. LWT-Food Science and Technology, 64(2): 1028-1035.

Yang, W., F. Guo and Z. Wan. 2013. Yield and size of oyster mushroom grown on rice/wheat straw basal substrate supplemented with cotton seed hull. Saudi journal of biological sciences, 20(4): 333-338.




DOI: https://doi.org/10.33866/phytopathol.036.01.1105

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Shazia Akram, Rizwan Khan, Owais Iqbal, Muhammad Nabeel, Sumbal Zaman, Nimra Khanzada, Muhammad Ali Khanzada

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

      
   
Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.
 Â