Muhammad R. Bashir, Ahsan M.U. Din, Ahsan Raza, Hafiz S.B Mustafa, Qamar A.T Khan, Muhammad Zubair, Ahmad N. Gill, Muhammad Anwar


The current research trial was conducted at Oilseeds Research Institute (ORI), Faisalabad for resistance/ susceptibility source against a devastating Brassica disease i.e. Alternaria leaf spot. In this field research trial, thirteen (13) varieties/ advanced lines of brassica were assessed during 2019-20 and 2020-21 by adopting statistical design namely RCBD (Randomized Complete Block Design) at the experimental research field of (ORI), Faisalabad under the umbrella of Ayub Agricultural Research Institute Faisalabad. In 2019-20, one advance line viz. RBJ-15013 exhibited resistant response with 8.00% disease incidence with rating 1 while six lines i.e. RBJ-15017, RBJ-16007, RBJ-17003, BRJ-17075, BRJ-17078 and Super Raya expressed moderately resistant reaction under rating 2. Likewise, four varieties/advanced lines exhibited moderately susceptible (MS) response whereas one advanced line KJ-294 expressed susceptible response (rating 4) and KJ-159 showed highly susceptible (HS) response by 85.67% disease incidence with rating 5. During 2020-21 one advanced line exhibited highly susceptible response with (81.70%) disease incidence with rating 5 while one of the advance line i.e. KJ-159 (71.80%) showed susceptible response (rating 4). Similarly, eight advanced lines viz. RBJ-15017, RBJ-17003, BRJ-17075, Super Raya, RBJ-17005, KJ-294, 18CBJ005 and KJ-284 exhibited moderately susceptible (MS) response (rating 3). Likewise, two variety/ advance lines RBJ-15013 and BRJ-17078 exhibited moderately resistant response whereas one of the advanced line i.e. RBJ-16007 (8.47%) showed resistant response (rating 1). It had been concluded that resistant varieties/ advanced lines are the most appropriate and economical disease management approach against Alternaria Leaf Spot Disease of brassica.


Source of Resistance/ susceptibility, Brassica juncia, Alternaria leaf spot, natural field conditions

Full Text:



Verma, P.R. and G.S. Saharan. 1994. Saskatoon Research Station Technical Bulletin. Monograph on Alternaria diseases of crucifers,.162:

Steel R.G.D., J.H. Torrie, D.A. Dicky, 1997 - Principles and procedures of statistics. A biometrical approaches. 3rd Ed. McGraw Hill Book Co. Inc. Singapore pp: 204- 227.

Ali, M., W. Muhammad and I. Ali. 2016. Yield of oilseed Brassica (napus and juncea) advanced lines as influenced by boron application. Soil Environment, 35(1): 30-34.

Bashir, M. R., A. Mahmood, M. Atiq, M. Yaseen, N. A. Rajput, M. Aftab, A. M. U. Din, M. Mohsan and Q. A. T. Khan. 2019. Assessment of genetic variability in mustard against Alternaria leaf spot disease through natural field conditions. Pakistan Journal of Phtyopathology, 31(2): 211-216.

Bhatt, R., R.P. Awasthi and A.K. Tewari. 2009. Management of downy mildew and white rust Diseases of mustard. Pantnagar Journal of Research, 7(1): 54-58

Bisht, K.S., M. Rana, K. Gairola, B.C. Sharma, A.K. Tewari and R.P. Awasthi. 2015. Screening of brassica germplasm for resistance to major diseases of rapeseed mustard. Supplement on Genetics and Plant Breeding. 10(4): 2111-2119

Cartea, M.E., M. Lena, M. Francisco, P. Velasco and J. Sadowski. 2011. Basic information on vegetable Brassica crops. Genetics, Genomics and Breeding of vegetables Brassicas, 1-33.

Dunder, M., A. Adhikari, B. Kontz, A. Varenhorst, T. Nleya, E. Byamukama, and F. Mathew. 2017. First report of Alternaria black spot caused by Alternaria alternata on Brassica carinata in South Dakota. Plant Disease, 101:1951.

FAOSTAT, Food and Agriculture Organization of United States of America. 2020. Production, Crops

Ghose, K., S. Dey, H. Barton, G. J. Loake and D. Basu. 2008. differential profiling of selected defense-related Genes induced on challenge with Alternaria brassicicola in resistant white mustard and their comparative expression pattern in susceptible India Mustard. Molecular Plant Pathology, 9(6): 763-775.

Al-lami, H. F. D., M. P. You and M. J. Barbetti. 2019. Role of foliage component and host age on severity of Alternaria leaf spot (caused by Alternaria japonica and A. brassicae) in canola (Brassica napus) and mustard (B. juncea) and yield loss in canola. Crop and Pasture Science, 70: 969–980.

Hansen. 1997. Somatic hybrids between brassica oleracea l. And sinapis alba l. With resistance to Alternaria brassicae (berk.) Theoretical and Applied Genetics, 94: 1078–1085.

Harloff, H. J., S. Lemcke, J. Mittasch, A. Frolov, J. G. Wu, F. Dreyer, G. Leckband and C. Jung. 2010. A mutation screening platform for rapeseed (Brassica napus L.) and the detection of sinapine biosynthesis mutants. Theoretical and Applied Genetics, 124: 957–969.

Higdon, J.V., B. Delage, D.E. Williams and R.H. Dashwood. 2007. Cruciferous vegetables and Human Cancer risk: epidemiological evidence and mechanistic basis. Pharmacological Research, 55: 224-236.

Humpherson-Jones, F. M. and K. Phelps. 1989. Climatic factors influencing spore production in Alternaria brassicae and Alternaria brassicicola. Annals of Applied Biology, 114: 449-458

Hussain, A. and R. N. Thakur. 1963. Some sources of resistance to Alternaria blight of rapeseed and mustard. Indian Oilseed Journal, 7: 259-261.

Hussain, T., M. A. Tariq, R. Ansar, M. Tariq and A. Sher. 2018. Screening of breeding lines of Brassica napus L. tolerant to grain shattering. Asian Journal of Agriculture and Biology, 259-264.

Kolte, S. J. 1985. Diseases of Annual Edible Oilseed Crops. Vol II: Rapeseed-Mustard and Sesame Diseases, CRC Press, Boca Raton, USA, 135.

Kristal, A.R. and J.W. Lampe. 2002. Brassica vegetables and prostate cancer risk: a review of the epidemiological evidence. Nature and Cancer, 42: 1-9.

Lampe, J. W. and S. Peterson. 2002. Brassica, biotransformation and cancer risk: genetic polymorphisms alter the preventive effects of cruciferous vegetables. The Journal of Nutrition, 132: 2991-2994.

Meena, P.D., R. P. Awasthi, C. Chattopadhyay, S. J. Kolte and A. Kumar. 2016. Alternaria blight: a chronic disease in rapeseed mustard. Journal of Oilseed Brassica. 1: 1-11.

Nowakowska M., M. Wrzesińska, P. Kaminski, W. Szczechura, M. Lichocka and M. Tartanus. 2019. Alternaria brassicicola–brassicaceae pathosystem: insights into the infection process and resistance mechanisms under optimized artificial bio-assay. European journal of Plant Pathology, 153: 131–151.

Dalel, S., R. Prasad and B. Bairwa. 2014 Screening of Brassica Germplasm and Bredding material against Alternaria brassicae causing Alternaria blight under Artificial condition, Journal of Pure and Applied Microbiology, 8(6): 91-102.

Saharan, G.S., N. Mehta, P.D. Meena and P. Dayal. 2016. ‘Alternaria diseases of crucifers: biology, ecology and disease management. Springer, 17-51.

Singh, D., R. Prasad and B. Bairwa. 2015. Screening of brassica germplasm and breeding material against Altenaria brassicae causing Alternaria blight under artificial condition. Journal of Pure and Applied Microbiology, 8(6): 1-5.

Sivasubramanian R., P. Deepak and K. Jagreet. 2019. Near-Complete Genome Assembly of Alternaria brassicae—A Necrotrophic Pathogen of Brassica Crops. Molecular Plant Microbe Interactions, 32: 928-930.

USDA.2008. States Department of Agriculture (USDA), Agricultural Research Service. National Nutrient Database for standard Reference, Release 21.

Subhani, A., M. Asif, M. Atiq, M. Imran, A. Hameed, A. Qadus, S. Ali, A. Sultan, M. Akmal, M. H. Haq, U. Farooq and N.A. Rajput. 2018. Progressive impact of Alternaria blight on antioxidants enzyme of mustard leaves after infection of Alternaria brassicae to induce resistance. International journal of Bioscience, 13(4): 417-426.

Verma N. and S. Verma. 2010. Alternaria diseases of vegetable crops and new approaches for its control. Asian Journal of Biological Science, 1: 681–692.

Woudenberg, J. H., J. Z. Groenewald, M. Binder and P. W. Crous. 2013. Alternaria redefined. Studies in Mycology, 75: 171-212.

Singh N. and P. Kalia. 2021. Screening of germplasm for Alternaria leaf spot resistance in yellow sarson. International Journal of Plant Protection, 1(2): 116-117.

Singh, K.P., P. Kumari and P.K. Rai, 2021. Current Status of the Disease-Resistant Gene(s)/QTLs, and Strategies for Improvement in Brassica juncea. Frontiers in Plant Science,12: 61740

Hassan, R. A., S. Ali, M. S. Zaheer, H. H. Ali, J. Iqbal, A. Habib, M. A. Nadeem and M. Z. Mumtaz. 2022. In-vitro and in-vivo evaluation of different fungicides against leaf blight causing fungus Alternaria cucumerina in bitter gourd. Journal of the Saudi Society of Agricultural Sciences, 21: 208-215.

Kumar, D., N. Maurya, Y.K. Bharati, A. Kumar, K. Kumar, K. Srivastava, G. Chand, C. Kushwaha, S.K. Singh and R.K. Mishra. 2014. Alternaria blight of oilseed Brassicas: A comprehensive review. African Journal of Microbiology and Research,



  • There are currently no refbacks.

Copyright (c) 2022 Muhammad Rizwan Bashir

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.