CONTROLLING STEM CANKER AND BLACK SCURF DISEASE COMPLEX ON POTATO PLANTS USING TRICHODERMA ISOLATES

Usamah A.A.A.A. Alshimaysawe, Akeel E. Mohammed, Sadeq M. Ali, Bashar K.H. Al-Gburi, Saba A.K. Al-fallooji

Abstract


Rhizoctonia solani, the causal agent of stem canker and black scurf disease on potato is a real threat to potato crop with up to 50% yield reduction. Current study was carried out to determine the effect of some Trichoderma isolates with different types of soil on the above diseases under laboratory and glasshouse conditions. Eight non-pathogenic strains of Trichoderma were isolated from potato roots and surrounding soil and examined for antagonism of Rhizoctonia diseases. Results showed that strains T. hamatum T8 and T. harzianum T5 had great potential as biocontrol agents for inhibiting the pathogen growth in dual culture and culture filtrate methods. The effect of different types of soils on the radial growth of R. solani AG-3PT in plates showed that Dorrigo soil inhibited the pathogen growth in laboratory by 68% as the colony diameter of pathogen recorded 2.8 cm which exceeds other soil types. While most Trichoderma strains that isolated from this area had a potential of protecting potatoes from the infection by the pathogen and acted as a biocontrol agent. Applying non-pathogenic Trichoderma isolates was the best method when inoculated the soil of pots with conidial suspensions which promote the growth of potato plants. T. harzianum (T5), T. hamatum (T6) and T. hamatum (T8) strains were eliminated stem canker and black scurf disease and improved potato plant growth in glasshouse experiment. The outcome of current study indicated the potential for utilizing Australian Trichoderma strains to reduce Rhizoctonia diseases on potato plants and increase productivity.

Keywords


Rhizoctonia solani AG-3PT, Sebago, Biocontrol agent, Growth promotion

Full Text:

PDF

References


Al-Gburi, B. K. H., F. H. Al-Sahaf, J. P. Del-Monte, F. A. Al-Fadhil and A. E. Mohammed. 2019. The effect of filaments and seeds on the virulence of Cuscuta campestris on eggplant. International Journal of Agricultural and Statistical Sciences, 15(1): 79-82.

Al-Gburi, B. K. H., S. K. A. Al-Fallooji, A. E. Mohammed and U. A. A. Alshimaysawe. 2022. Molecular identification of resistant melilotus indicus to weedicides and detection of associated Pseudomonas spp. International Journal of Agricultural and Statistical Sciences, 18: 1297-1301.

Alshimaysawe, U.A.A. 2018. Factors affecting biocontrol of Rhizoctonia diseases and growth promotion of potato by Trichoderma species. PhD thesis, University of New England, Australia.

Atkinson, D., M. K. Thornton and J.S. Miller. 2010. Development of Rhizoctonia solani on stems, stolons and tubers of potatoes. Effect of inoculum source. American Journal of Potato Research, 87(4): 374-381. Doi: 10.1007/s12230-010-9143-6.

Atkinson, D. 2005. Rhizoctonia solani AG-3 and potatoes: Evaluation of importance of source of inoculum and efficacy of in-furrow and seed piece treatments. Master Thesis, University of Idaho.

Aydin, M. H. 2018. Evaluation of some Trichoderma species in biological control of potato dry rot caused by Fusarium sambucinum fungal isolates. Applied Ecology and Environmental Research, 17(1): 533-546. Doi: http://dx.doi.org/10.15666/aeer/1701-533546.

Baijnath, S. 2012. Effect of inoculum source, inoculum pressure and cultivar on development of black scurf on potatoes in South Africa. Master thesis, University of Pretoria.

Banville, G. J. 1989. Yield losses and damage to potato plants caused by Rhizoctonia solani Kuhn. American Potato Journal, 66(12): 821-834.

Benitez, T., A.M. Rincon, M.C. Limon and A.C. Codon. 2004. Biocontrol mechanisms of Trichoderma strains. International Microbiology, 7(4): 249-260.

Cenis, J.L. 1992. Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Research, 20(9): 2380.

Chen, L.H., Y.Q. Cui, X.M. Yang, D.K. Zhao and Q.R. Shen. 2012. An antifungal compound from Trichoderma harzianum SQR-T037 effectively controls Fusarium wilt of cucumber in continuously cropped soil. Australasian Plant Pathology, 41(3): 239-245. Doi: 10.1007/s13313-012-0119-5.

Demirci, E. 2009. Biological control of Rhizoctonia solani on potato by Verticillium biguttatum. African Journal of Biotechnology, 8(11): 2503-2507.

Devi, R.N., B. Sinha and P.S. Devi. 2017. Native Trichoderma for the management of wire stem of mustard (Brassica spp) caused by Rhizoctonia solani. International Journal of Current Microbiology and Applied Sciences, 6(9): 2319-2328. Doi: org/10.20546/ijcmas.2017.609.284.

Elad, Y. and I. Chet. 1983. Improved selective media for isolation of Trichoderma spp. or Fusarium spp. Phytoparasitica, 11(1): 55-58. Doi: 10.1007/BF02980712.

Fiers, M., V. Edel-Hermann, C. Chatot, Y. Le Hingrat, C. Alabouvette and C. Steinberg. 2012. Potato soilborne diseases. A review. Agronomy for Sustainable Development, 32(1): 93-132.

Halifu, S., X. Deng, X. Song and R. Song. 2019. Effects of two Trichoderma strains on plant growth, rhizosphere soil nutrients, and fungal community of Pinus sylvestris var. mongolica annual seedlings. Forests, 10(9): 758. Doi: 10.3390/f10090758.

Ikeda, S., M. Shimizu , H. Takahashi and S. Takenaka. 2012. Biocontrol of black scurf on potato by seed tuber treatment with Pythium oligandrum. Biological control, 60(3): 297-304. Doi: 10.1016/j.biocontrol.2011.10.016.

Kredics, L., Z. Antal, L. Manczinger, A. Szekres, F. Kevei and E. Nagy. 2003. Influnce of environmental parameters on Trichoderma strains with biocontrol potential. Food Technology and Biotechnology, 41(1): 37-42.

Kreft, J.U., G. Booth and J. W. Wimpenny. 1998. BacSim, a simulator for individual based modeling of bacterial colony growth. Microbiology, 144(12): 3275-3287. Doi: 10.1099/00221287-144-12-3275.

Kumar, G., A. Maharshi, J. Patel, A. Mukherjee, H. B. Singh and B. K. Sarma. 2016. Trichoderma: a potential fungal antagonist to control plant diseases. SATSA Mukhapatra-Annual Technical, 21: 206-218.

Kumar, M., J. K. Singh, S. Kumar and A. Kumar. 2017. A comprehensive overview on black scurf of potato. International Journal of Current Microbiology and Applied Sciences, 6(10): 4981-4994. Doi: org/10.20546/ijcmas.2017.610.472.

Marfil, C.F., V. Hidalgo and R. W. Masuelli. 2015. In situ conservation of wild potato germplasm in Argentina: example and possibilities. Global Ecology and Conservation, 3: 461-476. Doi: org/10.1016/j.gecco.2015.01.009.

Mayo, S., S. Gutierrez, M. G. Malmierca, A. Lorenzana, M. P. Campelo, R. Hermosa and P. A. Casquero. 2015. Influence of Rhizoctonia solani and Trichoderma spp. in growth of bean (Phaseolus vulgaris L.) and in the induction of plant defense-related genes. Frontiers in Plant Science, 6: 685. Doi: 10.3389/fpls.2015.00685.

Meng, X., Y. Miao , Q. Liu, L. Ma, K. Guo, D. Liu, W. Ran and Q. Shen. 2019. TgSWO from Trichoderma guizhouense NJAU4742 promotes growth in cucumber plants by modifying the root morphology and the cell wall architecture. Microbial Cell Factories, 18(1): 48. Doi: 10.1186/s12934-019-1196-8.

Nusaibah, S.A. and H. Musa. 2019. Mechanism of Trichoderma spp. as biological control agent of the basal stem rot (BSR) disease of Elaeis guineensis. Annual Review of Intechnology, 1-7.

Rabeendran, N., D. J. Moot, E. E. Jones and A. Stewart. 2000. Inconsistent growth promotion of cabbage and lettuce from Trichoderma isolates. New Zealand Plant Protection, 53: 143-146.

Subhani, M.N., S. Talib, A. L. Sahi , S. Hussain, J. Iqbal and N. Hussain. 2013. Management of chickpea wilt caused by Fusarium oxysporum f. sp. ciceris through antagonistic microorganisms. Canadian Journal of Plant Protection, 1(1): 1-6.

Susiana, P., P. Achmadi, P. S. Retno, S. K. Rina and B. Kadarwati. 2018. The resistance of potatoes by application of Trichoderma viride antagonists fungus. EDP Sciences, 73: 06014. Doi: 10.1051/e3sconf/20187306014.

Taylor, F.I. 2013. Control of soil borne potato pathogens using Brassica spp. mediated biofumigation. PhD thesis, University of Glasgow.

Waghunde, R.R., R. M. Shelake and A. N. Sabalpara. 2016. Trichoderma: A significant fungus for agriculture and environment. African Journal of Agricultural Research, 11(22): 1952-1965. Doi: 10.5897/AJAR2015.10584.

Wimpenny, J. 1979. The growth and form of bacterial colonies. Microbiology, 114(2): 483-486. Doi: 10.1099/00221287-114-2-483.




DOI: https://doi.org/10.33866/phytopathol.036.01.0972

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Akeel Emad Mohammed, Usamah A. Alkarim Alshimaysawe, Sadeq Mohammed Ali, Bashar K Al-Gburi, sabaa H Alfalloji

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

      
   
Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.		 Â