Muhammad U. Yasin, Saba Saeed, Azhar Mustafa, Muhammad Iqbal


Citrus tristeza disease is the emerging devastating threat of citrus plants worldwide particularly in Punjab and KPK provinces of Pakistan. To evaluate the resistant cultivars, fourteen hundred leaf samples of 14 citrus cultivars were collected from Citrus Research Institute Sargodha, Pakistan. For the detection of tristeza disease, leaf sap was tested through a Double Antibody Sandwich Enzyme-linked Immunosorbent Assay technique. Reaction observed by yellow color development. The intensity of color development is directly proportional to the amount of pathogen enzyme trapped. Among all the cultivars highest was infection recorded in the Gil-Gil Citrus maxima i.e., 24% and lowest in seedless kinnow C. reticulata i.e., 3%. Seedless kinnow has a maximum level of resistance against citrus tristeza Clostero virus disease.


CTV Resistance LISA, incidence, Citrus nursery screening

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DOI: https://doi.org/10.33866/phytopathol.030.02.0591


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