Muhammad Irfan, Syed A. H. Naqvi, Rashida Perveen, Farah Naz, Ammarah Hasnain, Sobia Chohan


A survey was conducted for bacterial black spot disease in different districts of Punjab. Maximum disease incidence was observed in Multan followed by Muzaffar Garh and Khanewal while minimum disease incidence was noted in Jhang and Lodhran district of the Punjab. Thirty symptomatic samples were collected from multiple locations and associated pathogens were isolated. Bacterial isolates were inoculated on different mango cultivars to evaluate the level of resistance. None of the cultivar was resistant against the disease although Anwar Ratool and Late Chounsa were least affected. Eighteen isolates were grown on asparagine and nutrient agar with 5% glucose medium to check their variability which depicted round, convex, creamy white and yellow pigmented colonies with entire margins. Mucoid colony growth was found when inoculated on nutrient agar with 5% glucose. Biochemical tests showed that all bacterial isolates were gram negative, salt tolerant, oxidase negative and showed negative growth while grown on asparagines medium. On the basis of these tests bacteria were found as X. campestris. Although yellow pigmented isolates were found less aggressive as compared to creamy white ones.


Anacardiaceae, Occurrence, Biological characterization, Mangifera indica

Full Text:



Ah-You, N., L. Gagnevin, O. Pruvost, N. T. Myint and G. I. Johnson. 2007. First Report in Myanmar of Xanthomonas axonopodis pv. Mangiferae indicae Causing Mango Bacterial Canker on Mangifera indica. Plant Disease, 91: 1686-1686.

Ajila, C. M. and U. J. S. Prasada Rao. 2008. Protection against hydrogen peroxide induced oxidative damage in rat erythrocytes by Mangifera indica L. peel extract. Food and Chemical Toxicology, 46: 303-309.

Bally, I. 2006. Species profiles for pacific island agroforestry. Permanent Agricultural Resources (PAR)[Electronic resource].–2006.–URL: http://www. agroforestry. net/images/pdf.

Dayakar, B. and S. Gnanamanickam. 1996. Biochemical and pathogenic variation in strains of Xanthomonas campestris pv. Mangiferae indicae from Southern India. Indian Phytopathology, 49: 227-233.

Doidge, E. 1915. A bacterial disease of the mango. Bacillus mangiferae N.SP. Annals of Applied Biology, 2: 1-45.

Fukuda, T., K. Uehara, K. Azegami, H. Tabei and K. Nishiyama. 1990. Bacterial canker of mango in Japan caused by Xamthomonas campestris pv. Mangiferae indicae. Japanese Journal of Phytopathology, 56: 474-480.

Gagnevin, L. and O. Pruvost. 2001. Epidemiology and Control of Mango Bacterial Black Spot. Plant Disease, 85: 928-935.

Gagnevin, L., J. E. Leach and O. Pruvost. 1997. Genomic Variability of the Xanthomonas Pathovar mangiferae indicae, Agent of Mango Bacterial Black Spot. Applied and environmental microbiology, 63: 246-253.

Kishun, R. 1995. Detection and management of Xanthomonas campestris pv. Mangiferae indicae. Detection of Plant Pathogens and Their Management: 173-182.

Mahattanatawee, K., J. A. Manthey, G. Luzio, S. T. Talcott, K. Goodner and E. A. Baldwin. 2006. Total Antioxidant Activity and Fiber Content of Select Florida-Grown Tropical Fruits. Journal of Agricultural and Food Chemistry, 54: 7355-7363.

Manicom, B. Q. and F. M. Wallis. 1984. Further Characterization of Xanthomonas campestris pv. Mangiferae indicae. International Journal of Systematic Bacteriology, 34: 77-79.

McClung, L. S. 1985. Bergey's Manual of Systematic Bacteriology, Volume 1.: Edited by Noel R. Krieg. The Williams & Wilkins Co., Baltimore, 1984, 964 pp. $80.00. International Journal of Systematic Bacteriology, 35: 408-408.

Prakash, O., A. Misra and M. Raoof. 1994. Studies on mango bacterial canker disease. Bio. Memoirs, 20: 95-107.

Pruvost, Couteau, Perrier and Luisetti. 1998. Phenotypic diversity of Xanthomonas sp. mangiferae indicae. Journal of Applied Microbiology, 84: 115-124.

Pruvost, O., A. Couteau and J. Luisetti. 1992. Pepper Tree (Schinus terebenthifolius Radii), a New Host Plant for Xanthomonas campestris pv. Mangiferae indicae. Journal of Phytopathology, 135: 289-298.

Pruvost, O., A. Couteau, C. Verniere and J. Luisetti. 1993. Epiphytic Survival of Xanthomonas campestris pv. Mangiferae indicae on Mango Buds. Acta Horticulturae: 337-344.

Pruvost, O., A. couteau, X. perrier and J. Luisetti. 1995. Phenotypic diversity of Xanthomonas sp. mangiferae indicae. Journal of Applied Microbiology, 84:115-124.

Quimio, J.A. 1992. Annual report of the plant pathologist: July 17,1991 to July 16,1992. Enset Team Support Project Sidamo GamoGofa. Peasants Agricultural Development Program-PADEPIII. Awasa Research Center (IAR). Awasa, Ethiopia.

Sanders, G. M., J. A. Verschoor, S. van Wyngaard, L. Korsten and J. M. Kotzé. 1994. Production of monoclonal antibodies against Xanthomonas campestris pv. Mangiferae indicae and their use to investigate differences in virulence. Journal of Applied Bacteriology, 77: 509-518.

Schaad, N. W., J. B. Jones and W. Chun. 2001. Laboratory guide for the identification of plant pathogenic bacteria. American Phytopathological Society (APS Press).

Singh, U. P., D. P. Singh, M. Singh, S. Maurya, J. S. Srivastava, R. B. Singh and S. P. Singh. 2004. Characterization of phenolic compounds in some Indian mango cultivars. International Journal of Food Sciences and Nutrition, 55: 163-169.

DOI: https://doi.org/10.33866/phytopathol.029.02.0401


  • There are currently no refbacks.

Copyright (c) 2017 Muhammad Irfan, Syed A. H. Naqvi, Rashida Perveen, Farah Naz, Ammarah Hasnain, Sobia Chohan

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Pakistan Journal of Phytopathological
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.